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. 1994 Jul 19;91(15):7340–7344. doi: 10.1073/pnas.91.15.7340

Replicative intermediates of human papillomavirus type 11 in laryngeal papillomas: site of replication initiation and direction of replication.

K J Auborn 1, R D Little 1, T H Platt 1, M A Vaccariello 1, C L Schildkraut 1
PMCID: PMC44395  PMID: 8041792

Abstract

We have examined the structures of replication intermediates from the human papillomavirus type 11 genome in DNA extracted from papilloma lesions (laryngeal papillomas). The sites of replication initiation and termination utilized in vivo were mapped by using neutral/neutral and neutral/alkaline two-dimensional agarose gel electrophoresis methods. Initiation of replication was detected in or very close to the upstream regulatory region (URR; the noncoding, regulatory sequences upstream of the open reading frames in the papillomavirus genome). We also show that replication forks proceed bidirectionally from the origin and converge 180 degrees opposite the URR. These results demonstrate the feasibility of analysis of replication of viral genomes directly from infected tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bedell M. A., Hudson J. B., Golub T. R., Turyk M. E., Hosken M., Wilbanks G. D., Laimins L. A. Amplification of human papillomavirus genomes in vitro is dependent on epithelial differentiation. J Virol. 1991 May;65(5):2254–2260. doi: 10.1128/jvi.65.5.2254-2260.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brewer B. J., Fangman W. L. The localization of replication origins on ARS plasmids in S. cerevisiae. Cell. 1987 Nov 6;51(3):463–471. doi: 10.1016/0092-8674(87)90642-8. [DOI] [PubMed] [Google Scholar]
  3. Burnett S., Zabielski J., Moreno-Lopez J., Pettersson U. Evidence for multiple vegetative DNA replication origins and alternative replication mechanisms of bovine papillomavirus type 1. J Mol Biol. 1989 Mar 5;206(1):239–244. doi: 10.1016/0022-2836(89)90537-8. [DOI] [PubMed] [Google Scholar]
  4. Chiang C. M., Dong G., Broker T. R., Chow L. T. Control of human papillomavirus type 11 origin of replication by the E2 family of transcription regulatory proteins. J Virol. 1992 Sep;66(9):5224–5231. doi: 10.1128/jvi.66.9.5224-5231.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chiang C. M., Ustav M., Stenlund A., Ho T. F., Broker T. R., Chow L. T. Viral E1 and E2 proteins support replication of homologous and heterologous papillomaviral origins. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5799–5803. doi: 10.1073/pnas.89.13.5799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dartmann K., Schwarz E., Gissmann L., zur Hausen H. The nucleotide sequence and genome organization of human papilloma virus type 11. Virology. 1986 May;151(1):124–130. doi: 10.1016/0042-6822(86)90110-8. [DOI] [PubMed] [Google Scholar]
  7. Dasgupta S., Zabielski J., Simonsson M., Burnett S. Rolling-circle replication of a high-copy BPV-1 plasmid. J Mol Biol. 1992 Nov 5;228(1):1–6. doi: 10.1016/0022-2836(92)90485-3. [DOI] [PubMed] [Google Scholar]
  8. Dollard S. C., Wilson J. L., Demeter L. M., Bonnez W., Reichman R. C., Broker T. R., Chow L. T. Production of human papillomavirus and modulation of the infectious program in epithelial raft cultures. OFF. Genes Dev. 1992 Jul;6(7):1131–1142. doi: 10.1101/gad.6.7.1131. [DOI] [PubMed] [Google Scholar]
  9. Dubey D. D., Davis L. R., Greenfeder S. A., Ong L. Y., Zhu J. G., Broach J. R., Newlon C. S., Huberman J. A. Evidence suggesting that the ARS elements associated with silencers of the yeast mating-type locus HML do not function as chromosomal DNA replication origins. Mol Cell Biol. 1991 Oct;11(10):5346–5355. doi: 10.1128/mcb.11.10.5346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gamper H., Lehman N., Piette J., Hearst J. E. Purification of circular DNA using benzoylated naphthoylated DEAE-cellulose. DNA. 1985 Apr;4(2):157–164. doi: 10.1089/dna.1985.4.157. [DOI] [PubMed] [Google Scholar]
  11. Gissmann L., Wolnik L., Ikenberg H., Koldovsky U., Schnürch H. G., zur Hausen H. Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers. Proc Natl Acad Sci U S A. 1983 Jan;80(2):560–563. doi: 10.1073/pnas.80.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hammerschmidt W., Sugden B. Identification and characterization of oriLyt, a lytic origin of DNA replication of Epstein-Barr virus. Cell. 1988 Nov 4;55(3):427–433. doi: 10.1016/0092-8674(88)90028-1. [DOI] [PubMed] [Google Scholar]
  13. Krieg P., Amtmann E., Sauer G. The simultaneous extraction of high-molecular-weight DNA and of RNA from solid tumors. Anal Biochem. 1983 Oct 15;134(2):288–294. doi: 10.1016/0003-2697(83)90299-3. [DOI] [PubMed] [Google Scholar]
  14. Lack E. E., Vawter G. F., Smith H. G., Healy G. B., Lancaster W. D., Jenson A. B. Immunohistochemical localisation of human papillomavirus in squamous papillomas of the larynx. Lancet. 1980 Sep 13;2(8194):592–592. doi: 10.1016/s0140-6736(80)92027-9. [DOI] [PubMed] [Google Scholar]
  15. Lambert P. F. Papillomavirus DNA replication. J Virol. 1991 Jul;65(7):3417–3420. doi: 10.1128/jvi.65.7.3417-3420.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Linskens M. H., Huberman J. A. Ambiguities in results obtained with 2D gel replicon mapping techniques. Nucleic Acids Res. 1990 Feb 11;18(3):647–652. doi: 10.1093/nar/18.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lu J. Z., Sun Y. N., Rose R. C., Bonnez W., McCance D. J. Two E2 binding sites (E2BS) alone or one E2BS plus an A/T-rich region are minimal requirements for the replication of the human papillomavirus type 11 origin. J Virol. 1993 Dec;67(12):7131–7139. doi: 10.1128/jvi.67.12.7131-7139.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McCance D. J. Human papillomaviruses and cancer. Biochim Biophys Acta. 1986;823(3):195–205. doi: 10.1016/0304-419x(86)90002-8. [DOI] [PubMed] [Google Scholar]
  19. Meyers C., Frattini M. G., Hudson J. B., Laimins L. A. Biosynthesis of human papillomavirus from a continuous cell line upon epithelial differentiation. Science. 1992 Aug 14;257(5072):971–973. doi: 10.1126/science.1323879. [DOI] [PubMed] [Google Scholar]
  20. Mungal S., Steinberg B. M., Taichman L. B. Replication of plasmid-derived human papillomavirus type 11 DNA in cultured keratinocytes. J Virol. 1992 May;66(5):3220–3224. doi: 10.1128/jvi.66.5.3220-3224.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nawotka K. A., Huberman J. A. Two-dimensional gel electrophoretic method for mapping DNA replicons. Mol Cell Biol. 1988 Apr;8(4):1408–1413. doi: 10.1128/mcb.8.4.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Newlon C. S., Collins I., Dershowitz A., Deshpande A. M., Greenfeder S. A., Ong L. Y., Theis J. F. Analysis of replication origin function on chromosome III of Saccharomyces cerevisiae. Cold Spring Harb Symp Quant Biol. 1993;58:415–423. doi: 10.1101/sqb.1993.058.01.048. [DOI] [PubMed] [Google Scholar]
  23. Schvartzman J. B., Adolph S., Martín-Parras L., Schildkraut C. L. Evidence that replication initiates at only some of the potential origins in each oligomeric form of bovine papillomavirus type 1 DNA. Mol Cell Biol. 1990 Jun;10(6):3078–3086. doi: 10.1128/mcb.10.6.3078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stoler M. H., Broker T. R. In situ hybridization detection of human papillomavirus DNAs and messenger RNAs in genital condylomas and a cervical carcinoma. Hum Pathol. 1986 Dec;17(12):1250–1258. doi: 10.1016/s0046-8177(86)80569-x. [DOI] [PubMed] [Google Scholar]
  25. Strauss M., Jenson A. B. Human papillomavirus in various lesions of the head and neck. Otolaryngol Head Neck Surg. 1985 Jun;93(3):342–346. doi: 10.1177/019459988509300310. [DOI] [PubMed] [Google Scholar]
  26. Umek R. M., Linskens M. H., Kowalski D., Huberman J. A. New beginnings in studies of eukaryotic DNA replication origins. Biochim Biophys Acta. 1989 Jan 23;1007(1):1–14. doi: 10.1016/0167-4781(89)90123-1. [DOI] [PubMed] [Google Scholar]
  27. Ustav M., Ustav E., Szymanski P., Stenlund A. Identification of the origin of replication of bovine papillomavirus and characterization of the viral origin recognition factor E1. EMBO J. 1991 Dec;10(13):4321–4329. doi: 10.1002/j.1460-2075.1991.tb05010.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Waldeck W., Rösl F., Zentgraf H. Origin of replication in episomal bovine papilloma virus type 1 DNA isolated from transformed cells. EMBO J. 1984 Sep;3(9):2173–2178. doi: 10.1002/j.1460-2075.1984.tb02109.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yang L., Botchan M. Replication of bovine papillomavirus type 1 DNA initiates within an E2-responsive enhancer element. J Virol. 1990 Dec;64(12):5903–5911. doi: 10.1128/jvi.64.12.5903-5911.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yang L., Li R., Mohr I. J., Clark R., Botchan M. R. Activation of BPV-1 replication in vitro by the transcription factor E2. Nature. 1991 Oct 17;353(6345):628–632. doi: 10.1038/353628a0. [DOI] [PubMed] [Google Scholar]
  31. Yates J., Warren N., Reisman D., Sugden B. A cis-acting element from the Epstein-Barr viral genome that permits stable replication of recombinant plasmids in latently infected cells. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3806–3810. doi: 10.1073/pnas.81.12.3806. [DOI] [PMC free article] [PubMed] [Google Scholar]

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