Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1972 Feb;1(2):100–106. doi: 10.1128/aac.1.2.100

Automated Method for Determination of Penicillins, Cephalosporins, and Penicillinases

E Börje Lindström 1, Kurt Nordström 1
PMCID: PMC444176  PMID: 4618452

Abstract

The microiodometric assay of Novick for penicillinase was adapted to the Technicon Autoanalyzer system. With this automated method, it is possible to analyze almost 60 samples per hr. Two different procedures are described: in the first, the samples were incubated with substrate in the machine under standardized conditions; in the second, samples were incubated outside the Autoanalyzer and assayed at intervals for penicilloic acid. As little as 5 μm penicilloic acid could easily be detected in a sample of 0.4 ml. The reproducibility was very good. The hydrolysis of penicillin in a suspension of nongrowing bacteria as well as in a growing culture could also be followed. The automated method has been used both for kinetic studies and in screening for activity in eluates from column chromatography experiments.

Full text

PDF
103

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERTANI G. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol. 1951 Sep;62(3):293–300. doi: 10.1128/jb.62.3.293-300.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bomstein J., Evans W. G., Shepp J. M. Automated iodometric determination of penicillins. Ann N Y Acad Sci. 1965 Nov 9;130(2):589–595. doi: 10.1111/j.1749-6632.1965.tb12602.x. [DOI] [PubMed] [Google Scholar]
  3. Burman L. G., Nordström K., Boman H. G. Resistance of Escherichia coli to penicillins. V. Physiological comparison of two isogenic strains, one with chromosomally and one with episomally mediated ampicillin resistance. J Bacteriol. 1968 Aug;96(2):438–446. doi: 10.1128/jb.96.2.438-446.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eriksson-Grennberg K. G., Boman H. G., Jansson J. A., Thorén S. Resistance of Escherichia coli to Penicillins I. Genetic Study of Some Ampicillin-Resistant Mutants. J Bacteriol. 1965 Jul;90(1):54–62. doi: 10.1128/jb.90.1.54-62.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HAMILTON-MILLER J. M., SMITH J. T., KNOX R. The estimation of penicillins and penicillin destruction. J Pharm Pharmacol. 1963 Feb;15:81–91. doi: 10.1111/j.2042-7158.1963.tb12751.x. [DOI] [PubMed] [Google Scholar]
  6. JANSSON J. A. A DIRECT SPECTROPHOTOMETRIC ASSAY FOR PENICILLIN BETA-LACTAMASE (PENICILLINASE). Biochim Biophys Acta. 1965 Apr 26;99:171–172. doi: 10.1016/s0926-6593(65)80018-2. [DOI] [PubMed] [Google Scholar]
  7. Linström E. B., Boman H. G., Steele B. B. Resistance of Escherichia coli to penicillins. VI. Purification and characterization of the chromosomally mediated penicillinase present in ampA-containing strains. J Bacteriol. 1970 Jan;101(1):218–231. doi: 10.1128/jb.101.1.218-231.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. NOVICK R. P. Micro-iodometric assay for penicillinase. Biochem J. 1962 May;83:236–240. doi: 10.1042/bj0830236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PERRET C. J. Iodometric assay of penicillinase. Nature. 1954 Nov 27;174(4439):1012–1013. doi: 10.1038/1741012a0. [DOI] [PubMed] [Google Scholar]
  10. Sykes R. B., Nordström K. Microiodometric determination of beta-lactamase activity. Antimicrob Agents Chemother. 1972 Feb;1(2):94–99. doi: 10.1128/aac.1.2.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES