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. 1994 Aug 30;91(18):8353–8357. doi: 10.1073/pnas.91.18.8353

A 92-kDa human immunostimulatory protein.

E Fontan 1, E Briend 1, H Saklani-Jusforgues 1, J d'Alayer 1, J Vandekerckhove 1, R M Fauve 1
PMCID: PMC44604  PMID: 8078887

Abstract

We purified to apparent homogeneity a human urinary glycoprotein of 92 kDa (HGP.92) that, administered intravenously at 250 micrograms/kg, fully protected mice against a lethal inoculum of Listeria monocytogenes. Since HGP.92 protected scid mice, which lack B and T lymphocytes, this increased resistance to Listeria did not appear to be lymphocyte mediated. Furthermore, inflammatory macrophages incubated with 6 nM HGP.92 inhibited the growth of Lewis carcinoma cells in vitro. These two activities appeared to depend on an oligosaccharide moiety, as they were lost after N-Glycanase treatment of HGP.92. Thus, the biological activity of HGP.92 was in some way related to a glycan moiety.

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Selected References

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  1. Dawnay A., McLean C., Cattell W. R. The development of a radioimmunoassay for Tamm--Horsfall glycoprotein in serum. Biochem J. 1980 Mar 1;185(3):679–687. doi: 10.1042/bj1850679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dawnay A., McLean C., Cattell W. R. The development of a radioimmunoassay for Tamm--Horsfall glycoprotein in serum. Biochem J. 1980 Mar 1;185(3):679–687. doi: 10.1042/bj1850679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fauve R. M., Jusforgues H., Hevin B. Maintenance of granuloma macrophages in serum-free medium. J Immunol Methods. 1983 Nov 25;64(3):345–351. doi: 10.1016/0022-1759(83)90442-8. [DOI] [PubMed] [Google Scholar]
  4. Fletcher A. P., McLaughlin J. E., Ratcliffe W. A., Woods D. A. The chemical composition and electron microscopic appearance of a protein derived from urinary casts. Biochim Biophys Acta. 1970 Aug 21;214(2):299–308. doi: 10.1016/0005-2795(70)90007-3. [DOI] [PubMed] [Google Scholar]
  5. Fontan E., Fauve R. M. Inflammation and anti-tumor resistance. IV. Induction of cytostatic activity of murine peritoneal cells by a mouse granuloma protein. Int J Cancer. 1988 Aug 15;42(2):267–272. doi: 10.1002/ijc.2910420221. [DOI] [PubMed] [Google Scholar]
  6. Fontan E., Saklani-Jusforgues H., Fauve R. M. Immunostimulatory human urinary protein. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4358–4362. doi: 10.1073/pnas.89.10.4358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fontan E., Saklani H., Fauve R. M. Macrophage-induced cytotoxicity and anti-metastatic activity of a 43-kDa human urinary protein against the Lewis tumor. Int J Cancer. 1993 Jan 2;53(1):131–136. doi: 10.1002/ijc.2910530124. [DOI] [PubMed] [Google Scholar]
  8. Fukuoka S., Freedman S. D., Yu H., Sukhatme V. P., Scheele G. A. GP-2/THP gene family encodes self-binding glycosylphosphatidylinositol-anchored proteins in apical secretory compartments of pancreas and kidney. Proc Natl Acad Sci U S A. 1992 Feb 15;89(4):1189–1193. doi: 10.1073/pnas.89.4.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hession C., Decker J. M., Sherblom A. P., Kumar S., Yue C. C., Mattaliano R. J., Tizard R., Kawashima E., Schmeissner U., Heletky S. Uromodulin (Tamm-Horsfall glycoprotein): a renal ligand for lymphokines. Science. 1987 Sep 18;237(4821):1479–1484. doi: 10.1126/science.3498215. [DOI] [PubMed] [Google Scholar]
  10. Horton J. K., Davies M., Woodhead J. S., Weeks I. A new and rapid immunochemiluminometric assay for the measurement of Tamm-Horsfall glycoprotein. Clin Chim Acta. 1988 May 31;174(2):225–237. doi: 10.1016/0009-8981(88)90389-0. [DOI] [PubMed] [Google Scholar]
  11. Hoyer J. R., Seiler M. W. Pathophysiology of Tamm-Horsfall protein. Kidney Int. 1979 Sep;16(3):279–289. doi: 10.1038/ki.1979.130. [DOI] [PubMed] [Google Scholar]
  12. Kumar S., Jasani B., Hunt J. S., Moffat D. B., Asscher A. W. A system for accurate immunolocalization of Tamm-Horsfall protein in renal biopsies. Histochem J. 1985 Nov;17(11):1251–1258. doi: 10.1007/BF01002506. [DOI] [PubMed] [Google Scholar]
  13. Kumar S., Muchmore A. Tamm-Horsfall protein--uromodulin (1950-1990). Kidney Int. 1990 Jun;37(6):1395–1401. doi: 10.1038/ki.1990.128. [DOI] [PubMed] [Google Scholar]
  14. Liao Z., Grimshaw R. S., Rosenstreich D. L. Identification of a specific interleukin 1 inhibitor in the urine of febrile patients. J Exp Med. 1984 Jan 1;159(1):126–136. doi: 10.1084/jem.159.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McQueen E. G. The nature of urinary casts. J Clin Pathol. 1962 Jul;15(4):367–373. doi: 10.1136/jcp.15.4.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Muchmore A. V., Decker J. M. Uromodulin: a unique 85-kilodalton immunosuppressive glycoprotein isolated from urine of pregnant women. Science. 1985 Aug 2;229(4712):479–481. doi: 10.1126/science.2409603. [DOI] [PubMed] [Google Scholar]
  17. Muchmore A. V., Shifrin S., Decker J. M. In vitro evidence that carbohydrate moieties derived from uromodulin, an 85,000 dalton immunosuppressive glycoprotein isolated from human pregnancy urine, are immunosuppressive in the absence of intact protein. J Immunol. 1987 Apr 15;138(8):2547–2553. [PubMed] [Google Scholar]
  18. PIERCE C. H., DUBOS R. J., SCHAEFER W. B. Multiplication and survival of tubercle bacilli in the organs of mice. J Exp Med. 1953 Feb 1;97(2):189–206. doi: 10.1084/jem.97.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pennica D., Kohr W. J., Kuang W. J., Glaister D., Aggarwal B. B., Chen E. Y., Goeddel D. V. Identification of human uromodulin as the Tamm-Horsfall urinary glycoprotein. Science. 1987 Apr 3;236(4797):83–88. doi: 10.1126/science.3453112. [DOI] [PubMed] [Google Scholar]
  20. Ronco P., Brunisholz M., Geniteau-Legendre M., Chatelet F., Verroust P., Richet G. Physiopathologic aspects of Tamm-Horsfall protein: a phylogenetically conserved marker of the thick ascending limb of Henle's loop. Adv Nephrol Necker Hosp. 1987;16:231–249. [PubMed] [Google Scholar]
  21. Sikri K. L., Foster C. L., MacHugh N., Marshall R. D. Localization of Tamm-Horsfall glycoprotein in the human kidney using immuno-fluorescence and immuno-electron microscopical techniques. J Anat. 1981 Jun;132(Pt 4):597–605. [PMC free article] [PubMed] [Google Scholar]
  22. TAMM I., HORSFALL F. L., Jr A mucoprotein derived from human urine which reacts with influenza, mumps, and Newcastle disease viruses. J Exp Med. 1952 Jan;95(1):71–97. doi: 10.1084/jem.95.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. TAMM I., HORSFALL F. L., Jr Characterization and separation of an inhibitor of viral hemagglutination present in urine. Proc Soc Exp Biol Med. 1950 May;74(1):106–108. [PubMed] [Google Scholar]
  24. Yu C. L., Lin W. M., Liao T. S., Tsai C. Y., Sun K. H., Chen K. H. Tamm-Horsfall glycoprotein (THG) purified from normal human pregnancy urine increases phagocytosis, complement receptor expressions and arachidonic acid metabolism of polymorphonuclear neutrophils. Immunopharmacology. 1992 Nov-Dec;24(3):181–190. doi: 10.1016/0162-3109(92)90074-m. [DOI] [PubMed] [Google Scholar]

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