Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1994 Oct 25;91(22):10645–10649. doi: 10.1073/pnas.91.22.10645

A catalogue of genes in the cardiovascular system as identified by expressed sequence tags.

C C Liew 1, D M Hwang 1, Y W Fung 1, C Laurenssen 1, E Cukerman 1, S Tsui 1, C Y Lee 1
PMCID: PMC45078  PMID: 7938007

Abstract

The heart, which is composed of all the cellular components of the circulatory system, is a representative organ for obtaining genes expressed in the cardiovascular system in normal and disease states. We used partial sequences of cDNA clones, or expressed sequence tags, to identify and tag genes expressed in this organ. More than 3500 partial sequences representing > 3000 cDNA clones have been obtained from either the 5' or 3' end of inserts derived from human heart cDNA libraries. Of 3132 cDNA clones analyzed by sequence similarity searching against the GenBank/EMBL data bases, 1485 (47.4%) were found to represent additional, previously undiscovered genes, whereas 267 clones were matched to human brain expressed sequence tags. Clones matching to known genes were catalogued according to their putative structural and cellular functions. cDNA probes from reverse-transcribed mRNAs of fetal and adult hearts were used to study differential expression of selected clones in cardiac development. Cataloguing genes expressed in the heart may provide insight into the genes involved in health and cardiovascular disease.

Full text

PDF
10647

Images in this article

10647Image
on p.10647
10648Image
on p.10648

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams M. D., Dubnick M., Kerlavage A. R., Moreno R., Kelley J. M., Utterback T. R., Nagle J. W., Fields C., Venter J. C. Sequence identification of 2,375 human brain genes. Nature. 1992 Feb 13;355(6361):632–634. doi: 10.1038/355632a0. [DOI] [PubMed] [Google Scholar]
  2. Adams M. D., Kelley J. M., Gocayne J. D., Dubnick M., Polymeropoulos M. H., Xiao H., Merril C. R., Wu A., Olde B., Moreno R. F. Complementary DNA sequencing: expressed sequence tags and human genome project. Science. 1991 Jun 21;252(5013):1651–1656. doi: 10.1126/science.2047873. [DOI] [PubMed] [Google Scholar]
  3. Adams M. D., Kerlavage A. R., Fields C., Venter J. C. 3,400 new expressed sequence tags identify diversity of transcripts in human brain. Nat Genet. 1993 Jul;4(3):256–267. doi: 10.1038/ng0793-256. [DOI] [PubMed] [Google Scholar]
  4. Adams M. D., Soares M. B., Kerlavage A. R., Fields C., Venter J. C. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat Genet. 1993 Aug;4(4):373–380. doi: 10.1038/ng0893-373. [DOI] [PubMed] [Google Scholar]
  5. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  6. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  7. Gish W., States D. J. Identification of protein coding regions by database similarity search. Nat Genet. 1993 Mar;3(3):266–272. doi: 10.1038/ng0393-266. [DOI] [PubMed] [Google Scholar]
  8. Khan A. S., Wilcox A. S., Polymeropoulos M. H., Hopkins J. A., Stevens T. J., Robinson M., Orpana A. K., Sikela J. M. Single pass sequencing and physical and genetic mapping of human brain cDNAs. Nat Genet. 1992 Nov;2(3):180–185. doi: 10.1038/ng1192-180. [DOI] [PubMed] [Google Scholar]
  9. Liew C. C. A human heart cDNA library--the development of an efficient and simple method for automated DNA sequencing. J Mol Cell Cardiol. 1993 Aug;25(8):891–894. doi: 10.1006/jmcc.1993.1101. [DOI] [PubMed] [Google Scholar]
  10. Mesnard L., Samson F., Espinasse I., Durand J., Neveux J. Y., Mercadier J. J. Molecular cloning and developmental expression of human cardiac troponin T. FEBS Lett. 1993 Aug 9;328(1-2):139–144. doi: 10.1016/0014-5793(93)80981-y. [DOI] [PubMed] [Google Scholar]
  11. Okubo K., Hori N., Matoba R., Niiyama T., Fukushima A., Kojima Y., Matsubara K. Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression. Nat Genet. 1992 Nov;2(3):173–179. doi: 10.1038/ng1192-173. [DOI] [PubMed] [Google Scholar]
  12. Papadopoulos N., Nicolaides N. C., Wei Y. F., Ruben S. M., Carter K. C., Rosen C. A., Haseltine W. A., Fleischmann R. D., Fraser C. M., Adams M. D. Mutation of a mutL homolog in hereditary colon cancer. Science. 1994 Mar 18;263(5153):1625–1629. doi: 10.1126/science.8128251. [DOI] [PubMed] [Google Scholar]
  13. Wilcox A. S., Khan A. S., Hopkins J. A., Sikela J. M. Use of 3' untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implications for an expression map of the genome. Nucleic Acids Res. 1991 Apr 25;19(8):1837–1843. doi: 10.1093/nar/19.8.1837. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES