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. 1988 Dec 1;7(12):3643–3651. doi: 10.1002/j.1460-2075.1988.tb03245.x

Cell position and light influence C4 versus C3 patterns of photosynthetic gene expression in maize.

J A Langdale 1, I Zelitch 1, E Miller 1, T Nelson 1
PMCID: PMC454936  PMID: 2850171

Abstract

C4 plants such as maize partition photosynthetic activities in two morphologically distinct cell types, bundle sheath (BS) and mesophyll (M), which lie as concentric layers around veins. We show that both light and cell position relative to veins influence C4 photosynthetic gene expression. A pattern of gene expression characteristic of C3 plants [ribulose bisphosphate carboxylase (RuBPCase) and light-harvesting chlorophyll a/b binding protein in all photosynthetic cells] is observed in leaf-like organs such as husk leaves, which are sparsely vascularized. This pattern of gene expression reflects direct fixation of CO2 in the C3 photosynthetic pathway, as determined by O2 inhibition assays. Light induces a switch from C3-type to C4-type gene expression patterns in all leaves, primarily in cells that are close to a vein. We propose that light causes repression of RuBPCase expression in M cells, by a mechanism associated with the vascular system, and that this is an essential step in the induction of C4 photosynthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoyagi K., Bassham J. A. Appearance and accumulation of c(4) carbon pathway enzymes in developing maize leaves and differentiating maize a188 callus. Plant Physiol. 1986 Feb;80(2):322–333. doi: 10.1104/pp.80.2.322. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aoyagi K., Nakamoto H. Pyruvate, pi dikinase in bundle sheath strands as well as in mesophyll cells in maize leaves. Plant Physiol. 1985 Jul;78(3):661–664. doi: 10.1104/pp.78.3.661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Langdale J. A., Metzler M. C., Nelson T. The argentia mutation delays normal development of photosynthetic cell-types in Zea mays. Dev Biol. 1987 Jul;122(1):243–255. doi: 10.1016/0012-1606(87)90349-6. [DOI] [PubMed] [Google Scholar]
  4. Langdale J. A., Rothermel B. A., Nelson T. Cellular pattern of photosynthetic gene expression in developing maize leaves. Genes Dev. 1988 Jan;2(1):106–115. doi: 10.1101/gad.2.1.106. [DOI] [PubMed] [Google Scholar]
  5. Mangelsdorf P. C., Galinat W. C. THE TUNICATE LOCUS IN MAIZE DISSECTED AND RECONSTITUTED. Proc Natl Acad Sci U S A. 1964 Feb;51(2):147–150. doi: 10.1073/pnas.51.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Martineau B., Taylor W. C. Photosynthetic gene expression and cellular differentiation in developing maize leaves. Plant Physiol. 1985 Jun;78(2):399–404. doi: 10.1104/pp.78.2.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nagy F., Kay S. A., Chua N. H. Gene regulation by phytochrome. Trends Genet. 1988 Feb;4(2):37–42. doi: 10.1016/0168-9525(88)90064-9. [DOI] [PubMed] [Google Scholar]
  8. Nelson T., Harpster M. H., Mayfield S. P., Taylor W. C. Light-regulated gene expression during maize leaf development. J Cell Biol. 1984 Feb;98(2):558–564. doi: 10.1083/jcb.98.2.558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Perchorowicz J. T., Gibbs M. Carbon dioxide fixation and related properties in sections of the developing green maize leaf. Plant Physiol. 1980 May;65(5):802–809. doi: 10.1104/pp.65.5.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Peterson R. B., Zelitch I. Relationship between Net CO(2) Assimilation and Dry Weight Accumulation in Field-Grown Tobacco. Plant Physiol. 1982 Sep;70(3):677–685. doi: 10.1104/pp.70.3.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sheen J. Y., Bogorad L. Differential expression of C4 pathway genes in mesophyll and bundle sheath cells of greening maize leaves. J Biol Chem. 1987 Aug 25;262(24):11726–11730. [PubMed] [Google Scholar]
  12. Sheen J. Y., Bogorad L. Differential expression of six light-harvesting chlorophyll a/b binding protein genes in maize leaf cell types. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7811–7815. doi: 10.1073/pnas.83.20.7811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sheen J. Y., Bogorad L. Differential expression of the ribulose bisphosphate carboxylase large subunit gene in bundle sheath and mesophyll cells of developing maize leaves is influenced by light. Plant Physiol. 1985 Dec;79(4):1072–1076. doi: 10.1104/pp.79.4.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zelitch I. Pathways of carbon fixation in green plants. Annu Rev Biochem. 1975;44:123–145. doi: 10.1146/annurev.bi.44.070175.001011. [DOI] [PubMed] [Google Scholar]

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