Skip to main content
PMC home page
The EMBO Journal logoLink to The EMBO Journal
. 1988 Dec 1;7(12):4005–4010. doi: 10.1002/j.1460-2075.1988.tb03289.x

A novel replicon occurring naturally in Escherichia coli is a phage-plasmid hybrid.

W Seufert 1, R Lurz 1, W Messer 1
PMCID: PMC455010  PMID: 3061812

Abstract

A novel DNA replicon in Escherichia coli was identified. It is the smallest natural isolate (1282 bp) found so far. In the presence of phage M13 it grows as a filamentous single-stranded DNA phage. Contrary to previously identified mini-phages this replicon displays sequence homology only to parts of the M13 viral and complementary strand origin. In the absence of M13 this DNA replicates autonomously. The only gene (arp) of the replicon encodes a 32-kd protein, which is essential for autonomous replication. The host rep gene required for replication of single-stranded DNA phages is dispensable. Distinct replication mechanisms are thus involved during growth as defective phage or as autonomous plasmid.

Full text

PDF
4009

Images in this article

4005Image
on p.4005
4006Image
on p.4006
4008Image
on p.4008

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beck E., Zink B. Nucleotide sequence and genome organisation of filamentous bacteriophages fl and fd. Gene. 1981 Dec;16(1-3):35–58. doi: 10.1016/0378-1119(81)90059-7. [DOI] [PubMed] [Google Scholar]
  2. Chen E. Y., Seeburg P. H. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA. 1985 Apr;4(2):165–170. doi: 10.1089/dna.1985.4.165. [DOI] [PubMed] [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dotto G. P., Horiuchi K., Jakes K. S., Zinder N. D. Replication origin of bacteriophage f1. Two signals required for its function. J Mol Biol. 1982 Dec 5;162(2):335–343. doi: 10.1016/0022-2836(82)90530-7. [DOI] [PubMed] [Google Scholar]
  5. Dotto G. P., Zinder N. D. The morphogenetic signal of bacteriophage f1. Virology. 1983 Oct 15;130(1):252–256. doi: 10.1016/0042-6822(83)90136-8. [DOI] [PubMed] [Google Scholar]
  6. Enea V., Horiuchi K., Turgeon B. G., Zinder N. D. Physical map of defective interfering particles of bacteriophage f1. J Mol Biol. 1977 Apr 25;111(4):395–414. doi: 10.1016/s0022-2836(77)80061-2. [DOI] [PubMed] [Google Scholar]
  7. Geider K., Beck E., Schaller H. An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion. Proc Natl Acad Sci U S A. 1978 Feb;75(2):645–649. doi: 10.1073/pnas.75.2.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gray C. P., Sommer R., Polke C., Beck E., Schaller H. Structure of the orgin of DNA replication of bacteriophage fd. Proc Natl Acad Sci U S A. 1978 Jan;75(1):50–53. doi: 10.1073/pnas.75.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greenstein D., Horiuchi K. Interaction between the replication origin and the initiator protein of the filamentous phage f1. Binding occurs in two steps. J Mol Biol. 1987 Sep 20;197(2):157–174. doi: 10.1016/0022-2836(87)90115-x. [DOI] [PubMed] [Google Scholar]
  10. Griffith J., Kornberg A. Mini M13 bacteriophage: circular fragments of M13 DNA are replicated and packaged during normal infections. Virology. 1974 May;59(1):139–152. doi: 10.1016/0042-6822(74)90211-6. [DOI] [PubMed] [Google Scholar]
  11. Gros M. F., te Riele H., Ehrlich S. D. Rolling circle replication of single-stranded DNA plasmid pC194. EMBO J. 1987 Dec 1;6(12):3863–3869. doi: 10.1002/j.1460-2075.1987.tb02724.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kim M. H., Hines J. C., Ray D. S. Viable deletions of the M13 complementary strand origin. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6784–6788. doi: 10.1073/pnas.78.11.6784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kölling R., Gielow A., Seufert W., Kücherer C., Messer W. AsnC, a multifunctional regulator of genes located around the replication origin of Escherichia coli, oriC. Mol Gen Genet. 1988 Apr;212(1):99–104. doi: 10.1007/BF00322450. [DOI] [PubMed] [Google Scholar]
  14. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Meyer T. F., Geider K., Kurz C., Schaller H. Cleavage site of bacteriophage fd gene II-protein in the origin of viral strand replication. Nature. 1979 Mar 22;278(5702):365–367. doi: 10.1038/278365a0. [DOI] [PubMed] [Google Scholar]
  16. Peeters B. P., Peters R. M., Schoenmakers J. G., Konings R. N. Nucleotide sequence and genetic organization of the genome of the N-specific filamentous bacteriophage IKe. Comparison with the genome of the F-specific filamentous phages M13, fd and f1. J Mol Biol. 1985 Jan 5;181(1):27–39. doi: 10.1016/0022-2836(85)90322-5. [DOI] [PubMed] [Google Scholar]
  17. Ravetch J. V., Horiuchi K., Zinder N. D. DNA sequence analysis of the defective interfering particles of bacteriophage f1. J Mol Biol. 1979 Mar 5;128(3):305–318. doi: 10.1016/0022-2836(79)90090-1. [DOI] [PubMed] [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Valentine R. C., Shapiro B. M., Stadtman E. R. Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli. Biochemistry. 1968 Jun;7(6):2143–2152. doi: 10.1021/bi00846a017. [DOI] [PubMed] [Google Scholar]
  20. Viret J. F., Alonso J. C. Generation of linear multigenome-length plasmid molecules in Bacillus subtilis. Nucleic Acids Res. 1987 Aug 25;15(16):6349–6367. doi: 10.1093/nar/15.16.6349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  22. Zinder N. D., Horiuchi K. Multiregulatory element of filamentous bacteriophages. Microbiol Rev. 1985 Jun;49(2):101–106. doi: 10.1128/mr.49.2.101-106.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van Wezenbeek P. M., Hulsebos T. J., Schoenmakers J. G. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene. 1980 Oct;11(1-2):129–148. doi: 10.1016/0378-1119(80)90093-1. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES