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. 1988 May;7(5):1439–1447. doi: 10.1002/j.1460-2075.1988.tb02961.x

MAS1, a gene essential for yeast mitochondrial assembly, encodes a subunit of the mitochondrial processing protease.

C Witte 1, R E Jensen 1, M P Yaffe 1, G Schatz 1
PMCID: PMC458394  PMID: 3044780

Abstract

We have previously described a yeast mutant (mas1) that accumulates mitochondrial precursor proteins at high temperature and is deficient in the activity of a matrix-localized protease which cleaves presequences from mitochondrial precursor proteins. We have now cloned and sequenced the wild-type MAS1 gene and found that it encodes a subunit of the mitochondrial processing protease, that it is essential for cell viability and that the protein product participates in its own cleavage during import into mitochondria. The MAS1 protein is thus the first genetically defined component of the mitochondrial protein import pathway.

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