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. 1986 Apr;41(4):266–273. doi: 10.1136/thx.41.4.266

Raised immunoglobulin concentrations in bronchoalveolar lavage fluid of healthy granite workers.

W J Calhoun, J W Christman, W B Ershler, W G Graham, G S Davis
PMCID: PMC460308  PMID: 3738846

Abstract

Immunoglobulin concentrations and lymphocyte counts were determined in bronchoalveolar fluid obtained from nine symptomless, healthy, non-smoking granite workers (mean age 45.6, range 22-56 years) and nine normal, non-smoking, non industrial controls (mean age 22.8, range 21-32 years). The proteins were measured in unconcentrated lavage fluid by means of a solid phase, enzyme linked immunosorbent assay. IgG and IgA concentrations were three times greater in lavage fluid from granite workers than the samples from non-industrial controls (p less than 0.02). Eight of nine normal volunteers (89%) had no detectable IgM (less than 30 ng/ml) in the lavage fluid whereas eight of nine (89%) granite workers had detectable IgM (chi 2 = 8, p less than 0.01). Lymphocyte counts in lavage fluid from the workers were significantly greater (15.5%) than control counts (5.6%; p less than 0.05). The normal albumin concentration suggests that differences in permeability do not account for all of the increased immunoglobulin concentrations found in granite workers' lavage fluid and that some immunoglobulin is locally synthesised. It is concluded that occupational exposure to granite dust is associated with an increased proportion of lymphocytes and an increased concentration of immunoglobulin in lavage fluid that may reflect a subclinical immune inflammatory response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell D. Y., Haseman J. A., Spock A., McLennan G., Hook G. E. Plasma proteins of the bronchoalveolar surface of the lungs of smokers and nonsmokers. Am Rev Respir Dis. 1981 Jul;124(1):72–79. doi: 10.1164/arrd.1981.124.1.72. [DOI] [PubMed] [Google Scholar]
  2. Burger M., Hess M. W., Cottier H. The mechanism of SiO2 (Aerosil)-mediated enhancement of the proliferative response of mouse spleen lymphocytes to antigen and mitogens in a serum-free system. Eur J Immunol. 1983 Apr;13(4):331–335. doi: 10.1002/eji.1830130411. [DOI] [PubMed] [Google Scholar]
  3. CAPLAN A., PAYNE R. B., WITHEY J. L. A broader concept of Caplan's syndrome related to rheumatoid factors. Thorax. 1962 Sep;17:205–212. doi: 10.1136/thx.17.3.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Christman J. W., Emerson R. J., Graham W. G., Davis G. S. Mineral dust and cell recovery from the bronchoalveolar lavage of healthy Vermont granite workers. Am Rev Respir Dis. 1985 Aug;132(2):393–399. doi: 10.1164/arrd.1985.132.2.393. [DOI] [PubMed] [Google Scholar]
  5. Craighead J. E., Vallyathan N. V. Cryptic pulmonary lesions in workers occupationally exposed to dust containing silica. JAMA. 1980 Oct 24;244(17):1939–1941. [PubMed] [Google Scholar]
  6. Crystal R. G., Rennard S. I. Pulmonary connective tissue and environmental lung disease. Chest. 1981 Jul;80(1 Suppl):33–38. doi: 10.1378/chest.80.1_supplement.33s-a. [DOI] [PubMed] [Google Scholar]
  7. Davis G. S., Giancola M. S., Costanza M. C., Low R. B. Analyses of sequential bronchoalveolar lavage samples from healthy human volunteers. Am Rev Respir Dis. 1982 Oct;126(4):611–616. doi: 10.1164/arrd.1982.126.4.611. [DOI] [PubMed] [Google Scholar]
  8. Davis L. K., Wegman D. H., Monson R. R., Froines J. Mortality experience of Vermont granite workers. Am J Ind Med. 1983;4(6):705–723. doi: 10.1002/ajim.4700040604. [DOI] [PubMed] [Google Scholar]
  9. Doll N. J., Stankus R. P., Barkman H. W. Immunopathogenesis of asbestosis, silicosis, and coal workers' pneumoconiosis. Clin Chest Med. 1983 Jan;4(1):3–14. [PubMed] [Google Scholar]
  10. Doll N. J., Stankus R. P., Hughes J., Weill H., Gupta R. C., Rodriguez M., Jones R. N., Alspaugh M. A., Salvaggio J. E. Immune complexes and autoantibodies in silicosis. J Allergy Clin Immunol. 1981 Oct;68(4):281–285. doi: 10.1016/0091-6749(81)90152-4. [DOI] [PubMed] [Google Scholar]
  11. Graham W. G., O'Grady R. V., Dubuc B. Pulmonary function loss in Vermont granite workers. A long-term follow-up and critical reappraisal. Am Rev Respir Dis. 1981 Jan;123(1):25–28. doi: 10.1164/arrd.1981.123.1.25. [DOI] [PubMed] [Google Scholar]
  12. Heppleston A. G. Silicotic fibrogenesis: a concept of pulmonary fibrosis. Ann Occup Hyg. 1982;26(1-4):449–462. [PubMed] [Google Scholar]
  13. Jones R. N., Turner-Warwick M., Ziskind M., Weill H. High prevalence of antinuclear antibodies in sandblasters' silicosis. Am Rev Respir Dis. 1976 Mar;113(3):393–395. doi: 10.1164/arrd.1976.113.3.393. [DOI] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Low R. B., Davis G. S., Giancola M. S. Biochemical analyses of bronchoalveolar lavage fluids of healthy human volunteer smokers and nonsmokers. Am Rev Respir Dis. 1978 Nov;118(5):863–875. doi: 10.1164/arrd.1978.118.5.863. [DOI] [PubMed] [Google Scholar]
  16. Lowrie D. B. What goes wrong with the macrophage in silicosis? Eur J Respir Dis. 1982 May;63(3):180–182. [PubMed] [Google Scholar]
  17. Mandel M. A., Dvorak K. J., Worman L. W., DeCosse J. J. Immunoglobulin content in the bronchial washings of patients with benign and malignant pulmonary disease. N Engl J Med. 1976 Sep 23;295(13):694–698. doi: 10.1056/NEJM197609232951303. [DOI] [PubMed] [Google Scholar]
  18. Merrill W. W., Goodenberger D., Strober W., Matthay R. A., Naegel G. P., Reynolds H. Y. Free secretory component and other proteins in human lung lavage. Am Rev Respir Dis. 1980 Jul;122(1):156–161. doi: 10.1164/arrd.1980.122.1.156. [DOI] [PubMed] [Google Scholar]
  19. Merrill W., O'Hearn E., Rankin J., Naegel G., Matthay R. A., Reynolds H. Y. Kinetic analysis of respiratory tract proteins recovered during a sequential lavage protocol. Am Rev Respir Dis. 1982 Oct;126(4):617–620. doi: 10.1164/arrd.1982.126.4.617. [DOI] [PubMed] [Google Scholar]
  20. Musk A. W., Peters J. M., Wegman D. H., Fine L. J. Pulmonary function in granite dust exposure: a four-year follow-up. Am Rev Respir Dis. 1977 May;115(5):769–776. doi: 10.1164/arrd.1977.115.5.769. [DOI] [PubMed] [Google Scholar]
  21. Pernis B., Vigliani E. C. The role of macrophages and immunocytes in the pathogenesis of pulmonary diseases due to mineral dusts. Am J Ind Med. 1982;3(2):133–137. doi: 10.1002/ajim.4700030203. [DOI] [PubMed] [Google Scholar]
  22. Rankin J. A., Naegel G. P., Schrader C. E., Matthay R. A., Reynolds H. Y. Air-space immunoglobulin production and levels in bronchoalveolar lavage fluid of normal subjects and patients with sarcoidosis. Am Rev Respir Dis. 1983 Apr;127(4):442–448. doi: 10.1164/arrd.1983.127.4.442. [DOI] [PubMed] [Google Scholar]
  23. Reynolds H. Y., Fulmer J. D., Kazmierowski J. A., Roberts W. C., Frank M. M., Crystal R. G. Analysis of cellular and protein content of broncho-alveolar lavage fluid from patients with idiopathic pulmonary fibrosis and chronic hypersensitivity pneumonitis. J Clin Invest. 1977 Jan;59(1):165–175. doi: 10.1172/JCI108615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Reynolds H. Y., Newball H. H. Analysis of proteins and respiratory cells obtained from human lungs by bronchial lavage. J Lab Clin Med. 1974 Oct;84(4):559–573. [PubMed] [Google Scholar]
  25. Rodnan G. P., Benedek T. G., Medsger T. A., Jr, Cammarata R. J. The association of progressive systemic sclerosis (scleroderma) with coal miners' pneumoconiosis and other forms of silicosis. Ann Intern Med. 1967 Feb;66(2):323–334. doi: 10.7326/0003-4819-66-2-323. [DOI] [PubMed] [Google Scholar]
  26. Voller A., Bidwell D. E., Bartlett A. Enzyme immunoassays in diagnostic medicine. Theory and practice. Bull World Health Organ. 1976;53(1):55–65. [PMC free article] [PubMed] [Google Scholar]
  27. Warr G. A., Martin R. R., Sharp P. M., Rossen R. D. Normal human bronchial immunoglobulins and proteins: effects of cigarette smoking. Am Rev Respir Dis. 1977 Jul;116(1):25–30. doi: 10.1164/arrd.1977.116.1.25. [DOI] [PubMed] [Google Scholar]
  28. Weinberger S. E., Kelman J. A., Elson N. A., Young R. C., Jr, Reynolds H. Y., Fulmer J. D., Crystal R. G. Bronchoalveolar lavage in interstitial lung disease. Ann Intern Med. 1978 Oct;89(4):459–466. doi: 10.7326/0003-4819-89-4-459. [DOI] [PubMed] [Google Scholar]
  29. Ziskind M., Jones R. N., Weill H. Silicosis. Am Rev Respir Dis. 1976 May;113(5):643–665. doi: 10.1164/arrd.1976.113.5.643. [DOI] [PubMed] [Google Scholar]
  30. deShazo R. D. Current concepts about the pathogenesis of silicosis and asbestosis. J Allergy Clin Immunol. 1982 Jul;70(1):41–49. doi: 10.1016/0091-6749(82)90200-7. [DOI] [PubMed] [Google Scholar]

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