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. 1988 Mar;43(3):195–199. doi: 10.1136/thx.43.3.195

DNA ploidy in bronchopulmonary carcinoid tumours.

D J Jones 1, P S Hasleton 1, M Moore 1
PMCID: PMC461166  PMID: 3406904

Abstract

Fifty three bronchopulmonary carcinoid tumours were studied to assess the significance of DNA ploidy, determined by flow cytometry of paraffin embedded tissue. Twenty eight were typical carcinoid tumours and 25 well differentiated neuroendocrine carcinomas. Twenty seven were DNA diploid and 26 DNA aneuploid. DNA aneuploidy was significantly associated with histological features of increased malignant potential. Survival data were available for 43 patients. Of the 19 with DNA diploid tumours, 16 survived five years, compared with 14 of 24 with DNA aneuploid tumours--the difference being at the borderline of statistical significance. In a Cox multivariate regression analysis with other histological variables, DNA ploidy did not confer independent prognostic information. It is concluded that, although DNA aneuploidy as determined by flow cytometry is an indicator of increased malignant potential in bronchopulmonary carcinoid tumours, it does not provide clinically useful information additional to the results of routine histological examination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe S., Makimura S., Itabashi K., Nagai T., Tsuneta Y., Kawakami Y. Prognostic significance of nuclear DNA content in small cell carcinoma of the lung. Cancer. 1985 Oct 15;56(8):2025–2030. doi: 10.1002/1097-0142(19851015)56:8<2025::aid-cncr2820560823>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  2. Baildam A. D., Zaloudik J., Howell A., Barnes D. M., Turnbull L., Swindell R., Moore M., Sellwood R. A. DNA analysis by flow cytometry, response to endocrine treatment and prognosis in advanced carcinoma of the breast. Br J Cancer. 1987 May;55(5):553–559. doi: 10.1038/bjc.1987.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blöndal T., Arnorsson T., Bengtsson A., Wilander E. Nuclear DNA in carcinoid tumours of the lung. Eur J Respir Dis. 1983 May;64(4):298–305. [PubMed] [Google Scholar]
  4. Bunn P. A., Jr, Carney D. N., Gazdar A. F., Whang-Peng J., Matthews M. J. Diagnostic and biological implications of flow cytometric DNA content analysis in lung cancer. Cancer Res. 1983 Oct;43(10):5026–5032. [PubMed] [Google Scholar]
  5. Ewers S. B., Långström E., Baldetorp B., Killander D. Flow-cytometric DNA analysis in primary breast carcinomas and clinicopathological correlations. Cytometry. 1984 Jul;5(4):408–419. doi: 10.1002/cyto.990050419. [DOI] [PubMed] [Google Scholar]
  6. Falor W. H. Chromosomes in bronchial adenomas and in bronchogenic carcinomas. Am Rev Respir Dis. 1971 Aug;104(2):198–205. doi: 10.1164/arrd.1971.104.2.198. [DOI] [PubMed] [Google Scholar]
  7. Fisher E. R., Palekar A., Paulson J. D. Comparative histopathologic, histochemical, electron microscopic and tissue culture studies of bronchial carcinoids and oat cell carcinomas of lung. Am J Clin Pathol. 1978 Feb;69(2):165–172. doi: 10.1093/ajcp/69.2.165. [DOI] [PubMed] [Google Scholar]
  8. Fozard J. B., Quirke P., Dixon M. F., Giles G. R., Bird C. C. DNA aneuploidy in ulcerative colitis. Gut. 1986 Dec;27(12):1414–1418. doi: 10.1136/gut.27.12.1414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Friedlander M. L., Hedley D. W., Taylor I. W. Clinical and biological significance of aneuploidy in human tumours. J Clin Pathol. 1984 Sep;37(9):961–974. doi: 10.1136/jcp.37.9.961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goh H. S., Jass J. R. DNA content and the adenoma-carcinoma sequence in the colorectum. J Clin Pathol. 1986 Apr;39(4):387–392. doi: 10.1136/jcp.39.4.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gould V. E., Linnoila R. I., Memoli V. A., Warren W. H. Neuroendocrine cells and neuroendocrine neoplasms of the lung. Pathol Annu. 1983;18(Pt 1):287–330. [PubMed] [Google Scholar]
  12. Hammarberg C., Slezak P., Tribukait B. Early detection of malignancy in ulcerative colitis. A flow-cytometric DNA study. Cancer. 1984 Jan 15;53(2):291–295. doi: 10.1002/1097-0142(19840115)53:2<291::aid-cncr2820530218>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  13. Hasleton P. S., Gomm S., Blair V., Thatcher N. Pulmonary carcinoid tumours: a clinico-pathological study of 35 cases. Br J Cancer. 1986 Dec;54(6):963–967. doi: 10.1038/bjc.1986.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hedley D. W., Friedlander M. L., Taylor I. W., Rugg C. A., Musgrove E. A. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem. 1983 Nov;31(11):1333–1335. doi: 10.1177/31.11.6619538. [DOI] [PubMed] [Google Scholar]
  15. Moran R. E., Melamed M. R. Flow cytometric analysis of human lung cancer. Correlation with histologic type and stage. Anal Quant Cytol. 1984 Jun;6(2):99–104. [PubMed] [Google Scholar]
  16. Quirke P., Dixon M. F., Clayden A. D., Durdey P., Dyson J. E., Williams N. S., Bird C. C. Prognostic significance of DNA aneuploidy and cell proliferation in rectal adenocarcinomas. J Pathol. 1987 Apr;151(4):285–291. doi: 10.1002/path.1711510408. [DOI] [PubMed] [Google Scholar]
  17. Quirke P., Dyson J. E. Flow cytometry: methodology and applications in pathology. J Pathol. 1986 Jun;149(2):79–87. doi: 10.1002/path.1711490202. [DOI] [PubMed] [Google Scholar]
  18. Reid B. J., Haggitt R. C., Rubin C. E., Rabinovitch P. S. Barrett's esophagus. Correlation between flow cytometry and histology in detection of patients at risk for adenocarcinoma. Gastroenterology. 1987 Jul;93(1):1–11. [PubMed] [Google Scholar]
  19. Schutte B., Reynders M. M., Bosman F. T., Blijham G. H. Flow cytometric determination of DNA ploidy level in nuclei isolated from paraffin-embedded tissue. Cytometry. 1985 Jan;6(1):26–30. doi: 10.1002/cyto.990060106. [DOI] [PubMed] [Google Scholar]
  20. Tirindelli-Danesi D., Teodori L., Mauro F., Modini C., Botti C., Cicconetti F., Stipa S. Prognostic significance of flow cytometry in lung cancer. A 5-year study. Cancer. 1987 Aug 15;60(4):844–851. doi: 10.1002/1097-0142(19870815)60:4<844::aid-cncr2820600421>3.0.co;2-3. [DOI] [PubMed] [Google Scholar]
  21. Volm M., Drings P., Mattern J., Sonka J., Vogt-Moykopf I., Wayss K. Prognostic significance of DNA patterns and resistance-predictive tests in non-small cell lung carcinoma. Cancer. 1985 Sep 15;56(6):1396–1403. doi: 10.1002/1097-0142(19850915)56:6<1396::aid-cncr2820560630>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  22. Zimmerman P. V., Hawson G. A., Bint M. H., Parsons P. G. Ploidy as a prognostic determinant in surgically treated lung cancer. Lancet. 1987 Sep 5;2(8558):530–533. doi: 10.1016/s0140-6736(87)92923-0. [DOI] [PubMed] [Google Scholar]

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