Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1993 Jun 1;90(11):5095–5099. doi: 10.1073/pnas.90.11.5095

Molecular evolution of the Adh1 locus in the genus Zea.

B S Gaut 1, M T Clegg 1
PMCID: PMC46661  PMID: 8506356

Abstract

We sampled DNA sequences from the locus encoding alcohol dehydrogenase 1 (alcohol:NAD+ oxidoreductase, EC 1.1.1.1). Our sample represents Adh1 alleles from a wide geographic distribution of Zea mays (maize) and two species of teosinte (Zea luxurians and Zea diploperennis). Using these and previously published sequences, we analyze the molecular evolution of Adh1 in the genus Zea. We perform tests to characterize recombination and identify the putative parents of the recombinant Adh1-Cm allele. We also perform tests for selection but are unable to detect either a selective sweep or strong balancing selection at the Adh1 locus. We estimate that divergence times between teosinte and some maize alleles are approximately 1 million years, whereas divergence times between distantly related maize alleles are approximately 2 million years. We conclude that the common ancestor to the genus Zea was polymorphic at the Adh1 locus. On the basis of previous estimates of nucleotide diversity at other maize loci, it appears that the common ancestor to the genus Zea was polymorphic at many loci.

Full text

PDF
5095

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Begun D. J., Aquadro C. F. Levels of naturally occurring DNA polymorphism correlate with recombination rates in D. melanogaster. Nature. 1992 Apr 9;356(6369):519–520. doi: 10.1038/356519a0. [DOI] [PubMed] [Google Scholar]
  2. Brändén C. I., Eklund H., Cambillau C., Pryor A. J. Correlation of exons with structural domains in alcohol dehydrogenase. EMBO J. 1984 Jun;3(6):1307–1310. doi: 10.1002/j.1460-2075.1984.tb01967.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dennis E. S., Gerlach W. L., Pryor A. J., Bennetzen J. L., Inglis A., Llewellyn D., Sachs M. M., Ferl R. J., Peacock W. J. Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res. 1984 May 11;12(9):3983–4000. doi: 10.1093/nar/12.9.3983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eklund H., Nordström B., Zeppezauer E., Söderlund G., Ohlsson I., Boiwe T., Söderberg B. O., Tapia O., Brändén C. I., Akeson A. Three-dimensional structure of horse liver alcohol dehydrogenase at 2-4 A resolution. J Mol Biol. 1976 Mar 25;102(1):27–59. doi: 10.1016/0022-2836(76)90072-3. [DOI] [PubMed] [Google Scholar]
  5. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol. 1981;17(6):368–376. doi: 10.1007/BF01734359. [DOI] [PubMed] [Google Scholar]
  6. Freeling M. Allelic variation at the level of intragenic recombination. Genetics. 1978 May;89(1):211–224. doi: 10.1093/genetics/89.1.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Freeling M., Bennett D. C. Maize Adh1. Annu Rev Genet. 1985;19:297–323. doi: 10.1146/annurev.ge.19.120185.001501. [DOI] [PubMed] [Google Scholar]
  8. Gaut B. S., Clegg M. T. Molecular evolution of alcohol dehydrogenase 1 in members of the grass family. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2060–2064. doi: 10.1073/pnas.88.6.2060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goloubinoff P., Päbo S., Wilson A. C. Evolution of maize inferred from sequence diversity of an Adh2 gene segment from archaeological specimens. Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1997–2001. doi: 10.1073/pnas.90.5.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kreitman M., Hudson R. R. Inferring the evolutionary histories of the Adh and Adh-dup loci in Drosophila melanogaster from patterns of polymorphism and divergence. Genetics. 1991 Mar;127(3):565–582. doi: 10.1093/genetics/127.3.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kwiatowski J., Skarecky D., Hernandez S., Pham D., Quijas F., Ayala F. J. High fidelity of the polymerase chain reaction. Mol Biol Evol. 1991 Nov;8(6):884–887. doi: 10.1093/oxfordjournals.molbev.a040693. [DOI] [PubMed] [Google Scholar]
  12. MacRae A. F., Clegg M. T. Evolution of Ac and Dsl elements in select grasses (Poaceae). Genetica. 1992;86(1-3):55–66. doi: 10.1007/BF00133711. [DOI] [PubMed] [Google Scholar]
  13. Osterman J. C., Dennis E. S. Molecular analysis of the ADH1-Cm allele of maize. Plant Mol Biol. 1989 Aug;13(2):203–212. doi: 10.1007/BF00016138. [DOI] [PubMed] [Google Scholar]
  14. Sachs M. M., Dennis E. S., Gerlach W. L., Peacock W. J. Two Alleles of Maize ALCOHOL DEHYDROGENASE 1 Have 3' Structural and Poly(a) Addition Polymorphisms. Genetics. 1986 Jun;113(2):449–467. doi: 10.1093/genetics/113.2.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schwartz D., Laughner W. J. A molecular basis for heterosis. Science. 1969 Oct 31;166(3905):626–627. doi: 10.1126/science.166.3905.626. [DOI] [PubMed] [Google Scholar]
  17. Shattuck-Eidens D. M., Bell R. N., Neuhausen S. L., Helentjaris T. DNA sequence variation within maize and melon: observations from polymerase chain reaction amplification and direct sequencing. Genetics. 1990 Sep;126(1):207–217. doi: 10.1093/genetics/126.1.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Watterson G. A. On the number of segregating sites in genetical models without recombination. Theor Popul Biol. 1975 Apr;7(2):256–276. doi: 10.1016/0040-5809(75)90020-9. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES