Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1993 Jul 1;90(13):6047–6051. doi: 10.1073/pnas.90.13.6047

An ordered collection of Bacillus subtilis DNA segments cloned in yeast artificial chromosomes.

V Azevedo 1, E Alvarez 1, E Zumstein 1, G Damiani 1, V Sgaramella 1, S D Ehrlich 1, P Serror 1
PMCID: PMC46864  PMID: 8327482

Abstract

A collection of 772 Bacillus subtilis DNA segments was obtained by cloning in yeast artificial chromosomes. The B. subtilis inserts of 288 clones were mapped by hybridization using as probes 65 cloned genes and 188 isolated insert ends. In this way, 59 inserts were ordered in four contigs that cover > 98% of the B. subtilis chromosome. This ordered collection is now available for further genetic and physical analysis of the B. subtilis genome.

Full text

PDF
6047

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albertsen H. M., Abderrahim H., Cann H. M., Dausset J., Le Paslier D., Cohen D. Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents. Proc Natl Acad Sci U S A. 1990 Jun;87(11):4256–4260. doi: 10.1073/pnas.87.11.4256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Amjad M., Castro J. M., Sandoval H., Wu J. J., Yang M., Henner D. J., Piggot P. J. An SfiI restriction map of the Bacillus subtilis 168 genome. Gene. 1991 May 15;101(1):15–21. doi: 10.1016/0378-1119(91)90219-2. [DOI] [PubMed] [Google Scholar]
  3. Anand R., Villasante A., Tyler-Smith C. Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis. Nucleic Acids Res. 1989 May 11;17(9):3425–3433. doi: 10.1093/nar/17.9.3425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burgers P. M., Percival K. J. Transformation of yeast spheroplasts without cell fusion. Anal Biochem. 1987 Jun;163(2):391–397. doi: 10.1016/0003-2697(87)90240-5. [DOI] [PubMed] [Google Scholar]
  5. Burke D. T., Carle G. F., Olson M. V. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987 May 15;236(4803):806–812. doi: 10.1126/science.3033825. [DOI] [PubMed] [Google Scholar]
  6. Coulson A., Kozono Y., Lutterbach B., Shownkeen R., Sulston J., Waterston R. YACs and the C. elegans genome. Bioessays. 1991 Aug;13(8):413–417. doi: 10.1002/bies.950130809. [DOI] [PubMed] [Google Scholar]
  7. Coulson A., Sulston J., Brenner S., Karn J. Toward a physical map of the genome of the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7821–7825. doi: 10.1073/pnas.83.20.7821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Coulson A., Waterston R., Kiff J., Sulston J., Kohara Y. Genome linking with yeast artificial chromosomes. Nature. 1988 Sep 8;335(6186):184–186. doi: 10.1038/335184a0. [DOI] [PubMed] [Google Scholar]
  9. Dower W. J., Miller J. F., Ragsdale C. W. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 1988 Jul 11;16(13):6127–6145. doi: 10.1093/nar/16.13.6127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Garza D., Ajioka J. W., Burke D. T., Hartl D. L. Mapping the Drosophila genome with yeast artificial chromosomes. Science. 1989 Nov 3;246(4930):641–646. doi: 10.1126/science.2510296. [DOI] [PubMed] [Google Scholar]
  11. Green E. D., Olson M. V. Chromosomal region of the cystic fibrosis gene in yeast artificial chromosomes: a model for human genome mapping. Science. 1990 Oct 5;250(4977):94–98. doi: 10.1126/science.2218515. [DOI] [PubMed] [Google Scholar]
  12. Grill E., Somerville C. Construction and characterization of a yeast artificial chromosome library of Arabidopsis which is suitable for chromosome walking. Mol Gen Genet. 1991 May;226(3):484–490. doi: 10.1007/BF00260662. [DOI] [PubMed] [Google Scholar]
  13. Itaya M., Tanaka T. Complete physical map of the Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis method. J Mol Biol. 1991 Aug 5;220(3):631–648. doi: 10.1016/0022-2836(91)90106-g. [DOI] [PubMed] [Google Scholar]
  14. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  15. Kuspa A., Vollrath D., Cheng Y., Kaiser D. Physical mapping of the Myxococcus xanthus genome by random cloning in yeast artificial chromosomes. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8917–8921. doi: 10.1073/pnas.86.22.8917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lee J. T., Murgia A., Sosnoski D. M., Olivos I. M., Nussbaum R. L. Construction and characterization of a yeast artificial chromosome library for Xpter-Xq27.3: a systematic determination of cocloning rate and X-chromosome representation. Genomics. 1992 Mar;12(3):526–533. doi: 10.1016/0888-7543(92)90444-w. [DOI] [PubMed] [Google Scholar]
  17. Little R. D., Pilia G., Johnson S., D'Urso M., Schlessinger D. Yeast artificial chromosomes spanning 8 megabases and 10-15 centimorgans of human cytogenetic band Xq26. Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):177–181. doi: 10.1073/pnas.89.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Little R. D., Porta G., Carle G. F., Schlessinger D., D'Urso M. Yeast artificial chromosomes with 200- to 800-kilobase inserts of human DNA containing HLA, V kappa, 5S, and Xq24-Xq28 sequences. Proc Natl Acad Sci U S A. 1989 Mar;86(5):1598–1602. doi: 10.1073/pnas.86.5.1598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Monaco A. P., Walker A. P., Millwood I., Larin Z., Lehrach H. A yeast artificial chromosome contig containing the complete Duchenne muscular dystrophy gene. Genomics. 1992 Mar;12(3):465–473. doi: 10.1016/0888-7543(92)90436-v. [DOI] [PubMed] [Google Scholar]
  20. Niaudet B., Ehrlich S. D. In vitro genetic labeling of Bacillus subtilis cryptic plasmid pHV400. Plasmid. 1979 Jan;2(1):48–58. doi: 10.1016/0147-619x(79)90005-2. [DOI] [PubMed] [Google Scholar]
  21. Ochman H., Gerber A. S., Hartl D. L. Genetic applications of an inverse polymerase chain reaction. Genetics. 1988 Nov;120(3):621–623. doi: 10.1093/genetics/120.3.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Olson M. V., Dutchik J. E., Graham M. Y., Brodeur G. M., Helms C., Frank M., MacCollin M., Scheinman R., Frank T. Random-clone strategy for genomic restriction mapping in yeast. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7826–7830. doi: 10.1073/pnas.83.20.7826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Petit M. A., Mesas J. M., Noirot P., Morel-Deville F., Ehrlich S. D. Induction of DNA amplification in the Bacillus subtilis chromosome. EMBO J. 1992 Apr;11(4):1317–1326. doi: 10.1002/j.1460-2075.1992.tb05176.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Putzer H., Brakhage A. A., Grunberg-Manago M. Independent genes for two threonyl-tRNA synthetases in Bacillus subtilis. J Bacteriol. 1990 Aug;172(8):4593–4602. doi: 10.1128/jb.172.8.4593-4602.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Raymond-Denise A., Guillen N. Identification of dinR, a DNA damage-inducible regulator gene of Bacillus subtilis. J Bacteriol. 1991 Nov;173(22):7084–7091. doi: 10.1128/jb.173.22.7084-7091.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Roncero M. I. Genes controlling xylan utilization by Bacillus subtilis. J Bacteriol. 1983 Oct;156(1):257–263. doi: 10.1128/jb.156.1.257-263.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sargent C. A., Dunham I., Campbell R. D. Identification of multiple HTF-island associated genes in the human major histocompatibility complex class III region. EMBO J. 1989 Aug;8(8):2305–2312. doi: 10.1002/j.1460-2075.1989.tb08357.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sgaramella V., Ferretti L., Damiani G., Sora S. A procedure for cloning restriction fragments of DNA as single inserts in yeast artificial chromosomes. Biochem Int. 1990;20(3):503–510. [PubMed] [Google Scholar]
  29. Silverman G. A., Jockel J. I., Domer P. H., Mohr R. M., Taillon-Miller P., Korsmeyer S. J. Yeast artificial chromosome cloning of a two-megabase-size contig within chromosomal band 18q21 establishes physical linkage between BCL2 and plasminogen activator inhibitor type-2. Genomics. 1991 Feb;9(2):219–228. doi: 10.1016/0888-7543(91)90245-a. [DOI] [PubMed] [Google Scholar]
  30. Stragier P., Kunkel B., Kroos L., Losick R. Chromosomal rearrangement generating a composite gene for a developmental transcription factor. Science. 1989 Jan 27;243(4890):507–512. doi: 10.1126/science.2536191. [DOI] [PubMed] [Google Scholar]
  31. Tabata S., Higashitani A., Takanami M., Akiyama K., Kohara Y., Nishimura Y., Nishimura A., Yasuda S., Hirota Y. Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome. J Bacteriol. 1989 Feb;171(2):1214–1218. doi: 10.1128/jb.171.2.1214-1218.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Zagorec M., Steinmetz M. Construction of a derivative of Tn917 containing an outward-directed promoter and its use in Bacillus subtilis. J Gen Microbiol. 1991 Jan;137(1):107–112. doi: 10.1099/00221287-137-1-107. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES