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. 1976 Oct;31(5):558–564. doi: 10.1136/thx.31.5.558

Effect of chlorphentermine on the lipids of rat lungs.

J Gloster, D Heath, P Hasleton, P Harris
PMCID: PMC470475  PMID: 996817

Abstract

Chronic administration of chlorphentermine to rats resulted in a reduction of body weight compared to a normal control group. The weight of the heart, liver, kidney, and spleen was less in the treated group while the weight of the lungs was increased significantly. There was no change in the ratio of right ventricular to left ventricular weight in the rats treated with chlorphentermine, supporting the views that this drug does not cause pulmonary hypertension. Biochemical analysis showed that the increase in the weight of the lungs was due to the accumulation of phospholipid. All classes of phospholipid were affected, but particularly phosphatidyl choline, the tissue concentration of which increased nine times. Chlorphentermine also increased the proportion of palmitate present in pulmonary phosphatidyl choline. Histological examination of the lung after treatment with chlorphentermine showed evidence of this drug-induced lipidosis. No conclusion can as yet be reached as to the mechanism involved in the accumulation of phospholipid in the lung after chlorphentermine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. Chevalier G., Collet A. J. In vivo incorporation of choline- 3 H, leucine- 3 H and galactose- 3 H in alveolar type II pneumocytes in relation to surfactant synthesis. A quantitative radoautographic study in mouse by electron microscopy. Anat Rec. 1972 Nov;174(3):289–310. doi: 10.1002/ar.1091740303. [DOI] [PubMed] [Google Scholar]
  3. DUNCOMBE W. G. THE COLORIMETRIC MICRO-DETERMINATION OF NON-ESTERIFIED FATTY ACIDS IN PLASMA. Clin Chim Acta. 1964 Feb;9:122–125. doi: 10.1016/0009-8981(64)90004-x. [DOI] [PubMed] [Google Scholar]
  4. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  5. Franken G., Lüllmann H., Siegfriedt A. The occurrence of huge cells in pulmonary alveoli of rats treated by an anorexic drug. Arzneimittelforschung. 1970 Mar;20(3):417–417. [PubMed] [Google Scholar]
  6. Hallman M., Gluck L. Phosphatidylglycerol in lung surfactant. II. Subcellular distribution and mechanism of biosynthesis in vitro. Biochim Biophys Acta. 1975 Nov 21;409(2):172–191. doi: 10.1016/0005-2760(75)90152-6. [DOI] [PubMed] [Google Scholar]
  7. Heath D., Smith P., Hasleton P. S. Effects of chlorphentermine on the rat lung. Thorax. 1973 Sep;28(5):551–558. doi: 10.1136/thx.28.5.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Karabelnik D., Zbinden G. Drug-induced foam cell reactions in rats, II. Chemical analysis of lipids stored in lungs and foam cells after treatment with chlorphentermine, 5-[p-(fluoren-9-ylidenemethyl)phenyl]-2-piperidineethanol (RMI 10.393) and 1-chloramitriptyline. Hoppe Seylers Z Physiol Chem. 1975 Jul;356(7):1151–1160. doi: 10.1515/bchm2.1975.356.2.1151. [DOI] [PubMed] [Google Scholar]
  9. Lüllmann-Rauch R., Reil G. H., Rossen E., Seiler K. U. The ultrastructure of rat lung changes induced by an anorectic drug (chlorphentermine). Virchows Arch B Cell Pathol. 1972;11(2):167–181. doi: 10.1007/BF02889396. [DOI] [PubMed] [Google Scholar]
  10. Lüllmann H., Lüllmann-Rauch R., Reil G. H. Ultrastructural changes in adrenal, spleen and liver tissue, induced by chlorphentermine. Klin Wochenschr. 1973 Mar 15;51(6):284–285. doi: 10.1007/BF01467818. [DOI] [PubMed] [Google Scholar]
  11. Lüllmann H., Parwaresch M. R., Sattler M., Seiler K. U., Siegfriedt A. The effects of anorectic agents on the pulmonary pressure and morphology of rat lungs after chronic administration. Arzneimittelforschung. 1972 Dec;22(12):2096–2099. [PubMed] [Google Scholar]
  12. Lüllmann H., Rossen E., Seiler K. U. The pharmacokinetics of phentermine and chlorphentermine in chronically treated rats. J Pharm Pharmacol. 1973 Mar;25(3):239–243. doi: 10.1111/j.2042-7158.1973.tb10631.x. [DOI] [PubMed] [Google Scholar]
  13. Rooney S. A., Canavan P. M., Motoyama E. K. The identification of phosphatidylglycerol in the rat, rabbit, monkey and human lung. Biochim Biophys Acta. 1974 Jul 26;360(1):56–67. doi: 10.1016/0005-2760(74)90179-9. [DOI] [PubMed] [Google Scholar]
  14. STOECKENIUS W. Some electron microscopical observations on liquid-crystalline phases in lipid-water systems. J Cell Biol. 1962 Feb;12:221–229. doi: 10.1083/jcb.12.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schmien R., Seiler K. U., Wassermann O. Drug-induced phospholipidosis. I. Lipid composition and chlorphentermine content of rat lung tissue and alveolar macrophages after chronic treatment. Naunyn Schmiedebergs Arch Pharmacol. 1974;283(3):331–334. doi: 10.1007/BF00499192. [DOI] [PubMed] [Google Scholar]
  16. Seiler K. U., Wassermann O. Drug-induced phospholipidosis. II. Alterations in the phospholipid pattern of organs from mice, rats and guinea-pigs after chronic treatment with chlorphentermine. Naunyn Schmiedebergs Arch Pharmacol. 1975;288(2-3):261–268. doi: 10.1007/BF00500531. [DOI] [PubMed] [Google Scholar]
  17. Seydel J. K., Wassermann O. NMR-studies on the molecular basis of drug-induced phospholipidosis. Interaction between chlorphentermine and phosphatidylcholine. Naunyn Schmiedebergs Arch Pharmacol. 1973;279(2):207–210. doi: 10.1007/BF00503985. [DOI] [PubMed] [Google Scholar]
  18. Smith P., Heath D., Hasleton P. S. Electron microscopy of chlorphentermine lung. Thorax. 1973 Sep;28(5):559–566. doi: 10.1136/thx.28.5.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Smith P., Heath D., Hasleton P. Effects of prolonged administration of chlorphentermine on the rat lung. Pathol Eur. 1974;9(4):273–287. [PubMed] [Google Scholar]
  20. Smith P., Heath D., Moosavi H. The Clara cell. Thorax. 1974 Mar;29(2):147–163. doi: 10.1136/thx.29.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. VAN HANDEL E. Suggested modifications of the micro determination of triglycerides. Clin Chem. 1961 Jun;7:249–251. [PubMed] [Google Scholar]
  22. Vijeyaratnam G. S., Corrin B. Pulmonary histiocytosis simulating desquamative interstitial pneumonia in rats receiving oral iprindole. J Pathol. 1972 Oct;108(2):105–113. doi: 10.1002/path.1711080203. [DOI] [PubMed] [Google Scholar]

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