Skip to main content
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1965 Nov;18(6):790–792. doi: 10.1136/jcp.18.6.790

Rapid electrophoresis and quantitation of haemoglobins on cellulose acetate

A J Marengo-Rowe 1
PMCID: PMC473136  PMID: 5844210

Abstract

A rapid and reproducible electrophoretic method for the separation and quantitation of haemoglobins on cellulose acetate is described. The accuracy of the method and its possible sources of error are discussed. The normal range for haemoglobin A2 by this method is 1% to 3% of the total haemoglobin concentration. Blood samples from 32 thalassaemic patients showed haemoglobin A2 values of 3·5% to 7%.

Full text

PDF
792

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AFONSO E. Rapid determination of A2 hemoglobin by reverseflow electrophoresis on cellulose acetate membranes. Clin Chim Acta. 1962 Jul;7:545–549. doi: 10.1016/0009-8981(62)90097-9. [DOI] [PubMed] [Google Scholar]
  2. BARTLETT R. C. Rapid celluose acetate electrophoresis. II. Qualitative and quantitative hemoglobin fractionation. Clin Chem. 1963 Jun;9:325–329. [PubMed] [Google Scholar]
  3. FRIEDMAN H. S. A rapid screening test for abnormal hemoglobins. The detection of hemoglobin-A2. Clin Chim Acta. 1962 Jan;7:100–107. doi: 10.1016/0009-8981(62)90124-9. [DOI] [PubMed] [Google Scholar]
  4. GERALD P. S., DIAMOND L. K. The diagnosis of thalassemia trait by starch block electrophoresis of the hemoglobin. Blood. 1958 Jan;13(1):61–69. [PubMed] [Google Scholar]
  5. GRAHAM J. L., GRUNBAUM B. W. A rapid method for microelectrophoresis and quantitation of hemoglobins on cellulose acetate. Am J Clin Pathol. 1963 Jun;39:567–578. doi: 10.1093/ajcp/39.6.567. [DOI] [PubMed] [Google Scholar]
  6. HILGARTNER M. W., ERLANDSON M. E., WALDEN B. S., SMITH C. H. A comparison of the paper and starch block electrophoretic methods for determination of A2 hemoglobin. Am J Clin Pathol. 1961 Jan;35:26–30. doi: 10.1093/ajcp/35.1.26. [DOI] [PubMed] [Google Scholar]
  7. IBBOTSON R. N., CROMPTON B. A. Quantitative determination of haemoglobin A2 using paper electrophoresis. J Clin Pathol. 1961 Mar;14:164–166. doi: 10.1136/jcp.14.2.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KUNKEL H. G., WALLENIUS G. New hemoglobin in normal adult blood. Science. 1955 Aug 12;122(3163):288–288. doi: 10.1126/science.122.3163.288. [DOI] [PubMed] [Google Scholar]
  9. MASRI M. S., JOSEPHSON A. M., SINGER K. Starch block electrophoretic studies of human hemoglobin solutions. I. Technic and results in the normal adult. Blood. 1958 Jun;13(6):533–542. [PubMed] [Google Scholar]
  10. PETRAKIS N. L., DOHERTY M. A., GRUNUM B. W., ATCHLEY W. A. Cellulose acetate membranes for the electrophoretic demonstration of hemoglobin A2. Acta Haematol. 1962;27:96–103. doi: 10.1159/000206757. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES