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. 1993 Sep 15;90(18):8402–8406. doi: 10.1073/pnas.90.18.8402

What determines the strength of noncovalent association of ligands to proteins in aqueous solution?

S Miyamoto 1, P A Kollman 1
PMCID: PMC47364  PMID: 8378312

Abstract

Free energy perturbation methods using molecular dynamics have been used to calculate the absolute free energy of association of two ligand-protein complexes. The calculations reproduce the significantly more negative free energy of association of biotin to streptavidin, compared to N-L-acetyltryptophanamide/alpha-chymotrypsin. This difference in free energy of association is due to van der Waals/dispersion effects in the nearly ideally performed cavity that streptavidin presents to biotin, which involves four tryptophan residues.

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Selected References

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