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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1969 May;22(3):273–277. doi: 10.1136/jcp.22.3.273

The action of light on culture media

Pamela M Waterworth 1
PMCID: PMC474058  PMID: 5784977

Abstract

Failure of growth of staphylococci on nutrient agar plates was shown to be due to previous exposure to sunlight in an exceptionally well lit laboratory. Such plates inhibited the growth of Staph. aureus, Staph. albus, some Proteus species and Providence, but not that of various other bacteria. The affected species are found to be more susceptible to the action of hydrogen eroxide added to the medium.

The effect was produced in plates of five different nutrient agars, but not in two others which contained casein.

Plates awaiting inoculation should be protected from light; a white fabric blind was found not to be fully protective.

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Selected References

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  1. Anderson B. B., Cowan J. D. Effect of light on the Lactobacillus casei icrobiological assay. J Clin Pathol. 1968 Jan;21(1):85–87. doi: 10.1136/jcp.21.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]

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