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. 1969 May;22(3):334–339. doi: 10.1136/jcp.22.3.334

Immunofluorescent localization of Tamm-Horsfall mucoprotein in human kidney

J K McKenzie 1,2,3,1, E G McQueen 1,2,3
PMCID: PMC474080  PMID: 4891482

Abstract

A fluorescein-labelled antiserum to human Tamm-Horsfall mucoprotein applied to frozen human kidney sections gave strong specific labelling, mainly of cells of tubules in the outer medulla. By comparison with adjacent serial sections stained for alkaline phosphatase and succinic dehydrogenase, it is suggested that material reacting immunologically as Tamm-Horsfall muco-protein is found particularly in the cells of the ascending limb of the loop of Henle and the macula densa segment of the distal tubule.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyce W. H., King J. S., Fielden M. L. TOTAL NONDIALYZABLE SOLIDS (TNDS) IN HUMAN URINE. IX. IMMUNOCHEMICAL STUDIES OF THE R-1 "UROMUCOID" FRACTION. J Clin Invest. 1961 Aug;40(8 Pt 1-2):1453–1465. doi: 10.1172/JCI104376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CORNELIUS C. E., MIA A. S., ROSENFELD S. RUMINANT UROLITHIASIS. VII. STUDIES ON THE ORIGIN OF TAMM-HORSFALL URINARY MUCOPROTEIN AND ITS PRESENCE IN OVINE CALCULOUS MATRIX. Invest Urol. 1965 Mar;2:453–457. [PubMed] [Google Scholar]
  3. Friedman T. Immunofluorescent localization of Tamm-Horsfall mucoprotein. Experientia. 1966 Sep 15;22(9):624–625. doi: 10.1007/BF01895292. [DOI] [PubMed] [Google Scholar]
  4. KEUTEL H. J. LOCALIZATION OF UROMUCID IN HUMAN KIDNEY AND IN SECTIONS OF HUMAN KIDNEY STONE WITH THE FLUORESCENT ANTIBODY TECHNIQUE. J Histochem Cytochem. 1965 Mar;13:155–160. [PubMed] [Google Scholar]
  5. McQueen E. G. The nature of urinary casts. J Clin Pathol. 1962 Jul;15(4):367–373. doi: 10.1136/jcp.15.4.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. WACHSTEIN M. Histochemical staining reactions of the normally functioning and abnormal kidney. J Histochem Cytochem. 1955 Jul;3(4):246–270. doi: 10.1177/3.4.246. [DOI] [PubMed] [Google Scholar]

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