Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1974 Aug;27(8):608–614. doi: 10.1136/jcp.27.8.608

Diagnostic electron microscopy of faeces

II Acute gastroenteritis associated with reovirus-like particles

T H Flewett 1, Heather Davies 1, A S Bryden 1, M J Robertson 1
PMCID: PMC475412  PMID: 4214154

Abstract

Virus particles resembling reoviruses or orbiviruses were found in the faeces of 40 of 73 patients under 6 years of age with acute gastroenteritis and in faeces of only two babies among 31 patients under 6 years admitted to hospital with other diagnoses. In morphology the particles resemble orbiviruses more closely than reoviruses, but differ in appearance from the orbiviruses in having a smooth, circular outline with a well marked continuous rim as seen in negatively stained preparations. They appear not to be serologically related to reovirus types 1, 2, or 3 and may be members of a new group.

Full text

PDF
614

Images in this article

610Image
on p.610
610Image
on p.610
610Image
on p.610
611Image
on p.611
611Image
on p.611
611Image
on p.611
612Image
on p.612
612Image
on p.612
612Image
on p.612
612Image
on p.612

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet. 1974 Feb 2;1(7849):149–151. doi: 10.1016/s0140-6736(74)92440-4. [DOI] [PubMed] [Google Scholar]
  2. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet. 1973 Dec 8;2(7841):1281–1283. doi: 10.1016/s0140-6736(73)92867-5. [DOI] [PubMed] [Google Scholar]
  3. Borden E. C., Shope R. E., Murphy F. A. Physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus--a new taxonomic group. Physiocochemical and serological studies. J Gen Virol. 1971 Nov;13(2):261–271. doi: 10.1099/0022-1317-13-2-261. [DOI] [PubMed] [Google Scholar]
  4. Feinstone S. M., Kapikian A. Z., Purceli R. H. Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness. Science. 1973 Dec 7;182(4116):1026–1028. doi: 10.1126/science.182.4116.1026. [DOI] [PubMed] [Google Scholar]
  5. Fernelius A. L., Ritchie A. E., Classick L. G., Norman J. O., Mebus C. A. Cell culture adaptation and propagation of a reovirus-like agent of calf diarrhea from a field outbreak in Nebraska. Arch Gesamte Virusforsch. 1972;37(1):114–130. doi: 10.1007/BF01241157. [DOI] [PubMed] [Google Scholar]
  6. Flewett T. H., Bryden A. S., Davies H. Diagnostic electron microscopy of faeces. I. The viral flora of the faeces as seen by electron microscopy. J Clin Pathol. 1974 Aug;27(8):603–608. doi: 10.1136/jcp.27.8.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Flewett T. H., Bryden A. S., Davies H. Letter: Virus particles in gastroenteritis. Lancet. 1973 Dec 29;2(7844):1497–1497. doi: 10.1016/s0140-6736(73)92760-8. [DOI] [PubMed] [Google Scholar]
  8. Flewett T. H., Bryden A. S., Davies H., Woode G. N., Bridger J. C., Derrick J. M. Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet. 1974 Jul 13;2(7872):61–63. doi: 10.1016/s0140-6736(74)91631-6. [DOI] [PubMed] [Google Scholar]
  9. Kapikian A. Z., Gerin J. L., Wyatt R. G., Thornhill T. S., Chanock R. M. Density in cesium chloride of the 27 nm "8FIIa" particle associated with acute infectious nonbacterial gastroenteritis: determination by ultra-centrifugation and immune electron microscopy. Proc Soc Exp Biol Med. 1973 Mar;142(3):874–877. doi: 10.3181/00379727-142-37135. [DOI] [PubMed] [Google Scholar]
  10. Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., Chanock R. M. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972 Nov;10(5):1075–1081. doi: 10.1128/jvi.10.5.1075-1081.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murphy F. A., Borden E. C., Shope R. E., Harrison A. Physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus--a new taxonomic group. Electron microscopic studies. J Gen Virol. 1971 Nov;13(2):273–288. doi: 10.1099/0022-1317-13-2-273. [DOI] [PubMed] [Google Scholar]
  12. Murphy F. A., Coleman P. H., Harrison A. K., Gary G. W., Jr Colorado tick fever virus: an electron microscopic study. Virology. 1968 May;35(1):28–40. doi: 10.1016/0042-6822(68)90302-4. [DOI] [PubMed] [Google Scholar]
  13. Paver W. K., Ashley C. R., Caul E. O., Clarke S. K. A small virus in human faeces. Lancet. 1973 Feb 3;1(7797):237–240. doi: 10.1016/s0140-6736(73)90072-x. [DOI] [PubMed] [Google Scholar]
  14. Verwoerd D. W., Els H. J., De Villiers E. M., Huismans H. Structure of the bluetongue virus capsid. J Virol. 1972 Oct;10(4):783–794. doi: 10.1128/jvi.10.4.783-794.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Welch A. B., Thompson T. L. Physiochemical characterization of a neonatal calf diarrhea virus. Can J Comp Med. 1973 Jul;37(3):295–301. [PMC free article] [PubMed] [Google Scholar]
  16. Williams R. E. Benefit and mischief from commensal bacteria. J Clin Pathol. 1973 Nov;26(11):811–818. doi: 10.1136/jcp.26.11.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wood H. A. Viruses with double-stranded RNA genomes. J Gen Virol. 1973 Jun;20(Suppl):61–85. doi: 10.1099/0022-1317-20-Supplement-61. [DOI] [PubMed] [Google Scholar]
  18. Woode G. N., Bridger J. C., Hall G., Dennis M. J. The isolation of a reovirus-like agent associated with diarrhoea in colostrum-deprived calves in Great Britain. Res Vet Sci. 1974 Jan;16(1):102–105. [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES