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. 1993 Oct 15;90(20):9596–9600. doi: 10.1073/pnas.90.20.9596

Single amino acid substitutions uncouple the DNA binding and strand scission activities of Fok I endonuclease.

D S Waugh 1, R T Sauer 1
PMCID: PMC47616  PMID: 8415747

Abstract

Single alanine substitution mutations at Asp-450 or Asp-467 of the type IIS restriction enzyme Fok I have no effect on the ability of the enzyme to bind strongly and selectively to its recognition site but completely eliminate its ability to cleave either strand of substrate DNA. Since wild-type Fok I shows no kinetic preference or required order of strand cleavage, these results indicate that Fok I, which evidently functions as a monomer, uses a single catalytic center to cleave both strands of DNA. In this respect, Fok I may resemble other monomeric enzymes that cleave double-stranded DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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