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. 1970 May;23(4):299–303. doi: 10.1136/jcp.23.4.299

Assay of serum fibrin degradation products by agglutination-inhibition of coated erythrocytes

P C Das 1,2
PMCID: PMC476747  PMID: 5464330

Abstract

An immunological method for detecting fibrin degradation products by tanned cells agglutination has been standardized. The effects of different variables, such as cell concentration, pH, and length of incubation, and the specificity of the reaction, are described. The method is compared with other immunological techniques.

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Selected References

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  1. BANG N. U., FLETCHER A. P., ALKJAERSIG N., SHERRY S. Pathogenesis of the coagulation defect developing during pathological plasma proteolytic ("fibrinolytic") states. III. Demonstration of abnormal clot structure by electron microscopy. J Clin Invest. 1962 Apr;41:935–948. doi: 10.1172/JCI104548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Das P. C., Allan A. G., Woodfield D. G., Cash J. D. Fibrin degradation products in sera of normal subjects. Br Med J. 1967 Dec 23;4(5581):718–720. doi: 10.1136/bmj.4.5581.718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Das P. C., Cash J. D. Fibrinolysis at rest and after exercise in hepatic cirrhosis. Br J Haematol. 1969 Nov;17(5):431–443. doi: 10.1111/j.1365-2141.1969.tb01391.x. [DOI] [PubMed] [Google Scholar]
  5. Das P. C. Investigations and preparation of fibrinogencoated tan A/Das PC: Investigations and preparation of fibrinogen-coated tanned sheep red cells. J Clin Pathol. 1970 Mar;23(2):149–155. doi: 10.1136/jcp.23.2.149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FULTHORPE A. J. Factors influencing haemagglutination tests with tetanux antitoxin. Immunology. 1959 Apr;2(2):104–111. [PMC free article] [PubMed] [Google Scholar]
  7. Fisher S., Fletcher A. P., Alkjaersig N., Sherry S. Immunoelectrophoretic characterization of plasma fibrinogen derivatives in patients with pathological plasma proteolysis. J Lab Clin Med. 1967 Dec;70(6):903–922. [PubMed] [Google Scholar]
  8. Fox F. J., Jr, Wide L., Killander J., Gemzell C. Determination of fibrinogen and fibrinolysis products in human plasma by an immunochemical method. Scand J Clin Lab Invest. 1965;17(4):341–348. doi: 10.3109/00365516509077061. [DOI] [PubMed] [Google Scholar]
  9. LATALLO Z. S., BUDZYNSKI A. Z., LIPINSKI B., KOWALSKI E. INHIBITION OF THROMBIN AND OF FIBRIN POLYMERIZATION, TWO ACTIVITIES DERIVED FROM PLASMIN-DIGESTED FIBRINOGEN. Nature. 1964 Sep 12;203:1184–1185. doi: 10.1038/2031184a0. [DOI] [PubMed] [Google Scholar]
  10. LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
  11. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  12. Mackay A. V., Das P. C., Myerscough P. R., Cash J. D. Fibrinolytic components of human uterine arterial and venous blood. J Clin Pathol. 1967 May;20(3):227–230. doi: 10.1136/jcp.20.3.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Merskey C., Johnson A. J., Kleiner G. J., Wohl H. The defibrination syndrome: clinical features and laboratory diagnosis. Br J Haematol. 1967 Jul;13(4):528–549. doi: 10.1111/j.1365-2141.1967.tb00762.x. [DOI] [PubMed] [Google Scholar]
  14. Merskey C., Kleiner G. J., Johnson A. J. Quantitative estimation of split products of fibrinogen in human serum, relation to diagnosis and treatment. Blood. 1966 Jul;28(1):1–18. [PubMed] [Google Scholar]
  15. Murakami M. [An immunological method for demonstrating fibrin or fibrinogen degradation products in the circulating blood]. Nihon Ketsueki Gakkai Zasshi. 1965 Jun;28(3):341–344. [PubMed] [Google Scholar]
  16. NILEHN J. E., NILSSON I. M. DEMONSTRATION OF FIBRINOLYTIC SPLIT PRODUCTS IN HUMAN SERUM BY AN IMMUNOLOGICAL METHOD IN SPONTANEOUS AND INDUCED FIBRINOLYTIC STATES. Scand J Haematol. 1964;1:313–330. doi: 10.1111/j.1600-0609.1964.tb00029.x. [DOI] [PubMed] [Google Scholar]
  17. NUSSENZWEIG V., SELIGMANN M., GRABAR P. [The degradation products of human fibrinogen by plasmin. II. Immunological study: existence of native anti-fibrinogen antibodies possessing different specificities]. Ann Inst Pasteur (Paris) 1961 Apr;100:490–508. [PubMed] [Google Scholar]
  18. Niléhn J. E. Separation and estimation split "products" of fibrinogen and fibrin in human serum. Thromb Diath Haemorrh. 1967 Dec 31;18(3-4):487–498. [PubMed] [Google Scholar]
  19. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. II. Prog Allergy. 1962;6:30–154. doi: 10.1159/000313795. [DOI] [PubMed] [Google Scholar]
  20. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  21. Woodfield D. G., Cole S. K., Allan A. G., Cash J. D. Serum fibrin degradation products throughout normal pregnancy. Br Med J. 1968 Dec 14;4(5632):665–668. doi: 10.1136/bmj.4.5632.665. [DOI] [PMC free article] [PubMed] [Google Scholar]

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