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. 1970 Oct;23(7):586–589. doi: 10.1136/jcp.23.7.586

Separation of the proteins of cerebrospinal fluid using gel electrofocusing followed by electrophoresis

C Fossard 1, G Dale 1, A L Latner 1
PMCID: PMC476841  PMID: 5488785

Abstract

The technique previously applied to serum can, with minor modifications, be applied to cerebrospinal fluid. Good protein patterns have been obtained and similarities as well as differences have been demonstrated between cerebrospinal fluid and serum. The patterns obtained should prove useful in neurological diagnosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Awdeh Z. L., Williamson A. R., Askonas B. A. Isoelectric focusing in polyacrylamide gel and its application to immunoglobulins. Nature. 1968 Jul 6;219(5149):66–67. doi: 10.1038/219066a0. [DOI] [PubMed] [Google Scholar]
  2. BLAU J. N., HARRIS H., ROBSON E. B. Haptoglobins in cerebrospinal fluid. Clin Chim Acta. 1963 Mar;8:202–206. doi: 10.1016/0009-8981(63)90158-x. [DOI] [PubMed] [Google Scholar]
  3. Beeley J. A. Separation of human salivary proteins by iso-electric focusing in polyacrylamide gels. Arch Oral Biol. 1969 May;14(5):559–561. doi: 10.1016/0003-9969(69)90150-2. [DOI] [PubMed] [Google Scholar]
  4. CUNNINGHAM V. R. ANALYSIS OF "NATIVE" CEREBROSPINAL FLUID BY THE POLYACRYLAMIDE DISC ELECTROPHORESIS TECHNIQUE. J Clin Pathol. 1964 Mar;17:143–148. doi: 10.1136/jcp.17.2.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Catsimpoolas N. Micro isoelectric focusing in polyacrylamide gel columns. Anal Biochem. 1968 Dec;26(3):480–482. doi: 10.1016/0003-2697(68)90219-4. [DOI] [PubMed] [Google Scholar]
  6. Dale G., Latner A. L. Isoelectric focusing in polyacrylamide gels. Lancet. 1968 Apr 20;1(7547):847–848. doi: 10.1016/s0140-6736(68)90303-6. [DOI] [PubMed] [Google Scholar]
  7. Dale G., Latner A. L. Isoelectric focusing of serum proteins in acrylamide gels followed by electrophoresis. Clin Chim Acta. 1969 Apr;24(1):61–68. doi: 10.1016/0009-8981(69)90141-7. [DOI] [PubMed] [Google Scholar]
  8. Evans J. H., Quick D. T. Polyacrylamide gel electrophoresis of spinal fluid proteins, neurological disorders. Arch Neurol. 1966 Jan;14(1):64–72. doi: 10.1001/archneur.1966.00470070068008. [DOI] [PubMed] [Google Scholar]
  9. Fawcett J. S. Isoelectric fractionation of proteins on polyacrylamide gels. FEBS Lett. 1968 Jul;1(1):81–82. doi: 10.1016/0014-5793(68)80024-9. [DOI] [PubMed] [Google Scholar]
  10. Felgenhauer K., Bach S., Stammler A. Elektrophorese von Serum und Liquor cerebrospinalis in Polyacrylamid-Gel. Klin Wochenschr. 1967 Apr 1;45(7):371–377. doi: 10.1007/BF01738746. [DOI] [PubMed] [Google Scholar]
  11. GAVRILESCO K., COURCON J., HILLION P., URIEL J., LEWIN J., GRABAR P. Etude du liquide céphalo-rachidien humain normal par la méthode immuno-électrophorétique. Bull Soc Chim Biol (Paris) 1955;37(7-8):803–807. [PubMed] [Google Scholar]
  12. GRABAR P., BURTIN P. Etude immunochimique de la sidérophiline. Bull Soc Chim Biol (Paris) 1955;37(7-8):797–802. [PubMed] [Google Scholar]
  13. Kabat E. A., Moore D. H., Landow H. AN ELECTROPHORETIC STUDY OF THE PROTEIN COMPONENTS IN CEREBROSPINAL FLUID AND THEIR RELATIONSHIP TO THE SERUM PROTEINS. J Clin Invest. 1942 Sep;21(5):571–577. doi: 10.1172/JCI101335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MONSEU G., CUMINGS J. N. POLYACRYLAMIDE DISC ELECTROPHORESIS OF THE PROTEINS OF CEREBROSPINAL FLUID AND BRAIN. J Neurol Neurosurg Psychiatry. 1965 Feb;28:56–60. doi: 10.1136/jnnp.28.1.56. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PETTE D., STUPP I. [The tau-fraction in cerebrospinal fluid]. Klin Wochenschr. 1960 Feb 1;38:109–110. doi: 10.1007/BF02189073. [DOI] [PubMed] [Google Scholar]
  16. SMITHIES O., WALKER N. F. Notation for serum-protein groups and the genes controlling their inheritance. Nature. 1956 Sep 29;178(4535):694–695. doi: 10.1038/178694a0. [DOI] [PubMed] [Google Scholar]
  17. Shapiro H. D., Miller K. D., Harris A. H. Low-pH disc electrophoresis of spinal fluid; changes in multiple sclerosis. Exp Mol Pathol. 1967 Dec;7(3):362–365. doi: 10.1016/0014-4800(67)90047-0. [DOI] [PubMed] [Google Scholar]
  18. Wrigley C. W. Analytical fractionation of plant and animal proteins by gel electrofocusing. J Chromatogr. 1968 Aug 27;36(3):362–365. doi: 10.1016/s0021-9673(01)92959-0. [DOI] [PubMed] [Google Scholar]

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