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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1970 Oct;23(7):594–598. doi: 10.1136/jcp.23.7.594

A fluorimetric and enzymatic method for the estimation of serum total bile acids

G M Murphy 1,2, Barbara H Billing 1,2, D N Baron 1,2
PMCID: PMC476843  PMID: 4320681

Abstract

The enzymatic technique using 3α-hydroxysteroid dehydrogenase for determining bile acids in blood has been modified by measuring the reduced nicotinamide adenine dinucleotide fluorimetrically. The increased sensitivity attained enables the concentration of total bile acids in serum to be estimated using 3 ml for normal subjects and 1 ml for jaundiced patients. The range of normal values in serum was found to be 0-4·7 μmol/litre for males and 1·0-8·2 μmol/litre for females.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Frosch B., Wagener H. Quantitative determination of conjugated bile acids in serum in acute hepatitis. Nature. 1967 Jan 28;213(5074):404–405. doi: 10.1038/213404a0. [DOI] [PubMed] [Google Scholar]
  2. Green W. G., Israelstam G. F. Increased sensitivity in fluorometric determinations of nicotinamide nucleotide coenzymes. Can J Biochem. 1968 Apr;46(4):390–392. doi: 10.1139/o68-059. [DOI] [PubMed] [Google Scholar]
  3. Gregg J. A. New solvent systems for thin-layer chromatography of bile acids. J Lipid Res. 1966 Jul;7(4):579–581. [PubMed] [Google Scholar]
  4. HURLOCK B., TALALAY P. Principles of the enzymatic measurement of steroids. J Biol Chem. 1957 Jul;227(1):37–52. [PubMed] [Google Scholar]
  5. IWATA T., YAMASAKI K. ENZYMATIC DETERMINATION AND THIN-LAYER CHROMATOGRAPHY OF BILE ACIDS IN BLOOD. J Biochem. 1964 Nov;56:424–431. doi: 10.1093/oxfordjournals.jbchem.a128013. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROBERTS N. R., KAPPHAHN J. I. The fluorometric measurement of pyridine nucleotides. J Biol Chem. 1957 Feb;224(2):1047–1064. [PubMed] [Google Scholar]
  7. Lewin M. R., Wills M. R., Baron D. N. Ultramicrofluorimetric determination of calcium in plasma. J Clin Pathol. 1969 Mar;22(2):222–225. doi: 10.1136/jcp.22.2.222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MARCUS P. I., TALALAY P. Induction and purification of alpha- and beta-hydroxysteroid dehydrogenases. J Biol Chem. 1956 Feb;218(2):661–674. [PubMed] [Google Scholar]
  9. Makino I., Nakagawa S., Mashimo K. Conjugated and unconjugated serum bile acid levels n patients with hepatobiliary diseases. Gastroenterology. 1969 Jun;56(6):1033–1039. [PubMed] [Google Scholar]
  10. Panveliwalla D., Lewis B., Wootton I. D., Tabaqchali S. Determination of individual bile acids in biological fluids by thin-layer chromatography and fluorimetry. J Clin Pathol. 1970 May;23(4):309–314. doi: 10.1136/jcp.23.4.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SANDBERG D. H., SJOEVALL J., SJOEVALL K., TURNER D. A. MEASUREMENT OF HUMAN SERUM BILE ACIDS BY GAS-LIQUID CHROMATOGRAPHY. J Lipid Res. 1965 Apr;6:182–192. [PubMed] [Google Scholar]
  12. Turnberg L. A., Anthony-Mote A. The quantitative determination of bile salts in bile using thin-layer chromatography and 3 alpha-hydroxysteroid dehydrogenase. Clin Chim Acta. 1969 May;24(2):253–259. doi: 10.1016/0009-8981(69)90321-0. [DOI] [PubMed] [Google Scholar]

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