Abstract
Gonococci suspended in 1% proteose peptone containing 8% glycerol can be snap frozen in liquid nitrogen without detectable loss on thawing. This recovery rate has allowed the use of frozen organisms as the starting inoculum for the bulk growth of gonococci and to preserve gonococci both in urethral pus and after primary subculture for use in studies on gonococcal virulence.
The method described for freezing urethral pus on charcoal swabs should make it possible to transport infected specimens from areas lacking adequate laboratory facilities.
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Selected References
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- KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kocka F. E., Bretz W. H. Survival of Escherichia coli after storage in various frozen menstrua. Antonie Van Leeuwenhoek. 1969;35(1):65–76. doi: 10.1007/BF02219117. [DOI] [PubMed] [Google Scholar]
- PIERCE C. H., DUBOS R. J., SCHAEFER W. B. Multiplication and survival of tubercle bacilli in the organs of mice. J Exp Med. 1953 Feb 1;97(2):189–206. doi: 10.1084/jem.97.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHITE L. A., KELLOGG D. S., Jr NEISSERIA GONORRHOEAE IDENTIFICATION IN DIRECT SMEARS BY A FLUORESCENT ANTIBODY-COUNTERSTAIN METHOD. Appl Microbiol. 1965 Mar;13:171–174. doi: 10.1128/am.13.2.171-174.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watt P. J. The fate of gonococci in polymorphonuclear leucocytes. J Med Microbiol. 1970 Aug;3(3):501–509. doi: 10.1099/00222615-3-3-501. [DOI] [PubMed] [Google Scholar]