Abstract
To facilitate the diagnosis of recent rubella infection, rubella haemagglutination inhibiting antibody has been determined in four fractions obtained by Sephadex G-200 gel filtration of samples of serum. All the 21 samples collected at the convalescent stage of the disease had varying proportions of haemagglutination inhibiting antibody in fraction 1, representing the major portion of IgM antibody whereas all but three out of 22 sera from persons with no history of recent rubella had negative titres in this fraction. The haemagglutination inhibiting titres in the three positive sera in the second group was very low as compared to the other fractions. Fractionation of sera on a Sephadex G-200 column coupled with the rubella haemagglutination inhibition test can, therefore, be used to diagnose recent rubella infection.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Banatvala J. E., Best J. M., Kennedy E. A., Smith E. E., Spence M. E. A serological method for demonstrating recent infection by rubella virus. Br Med J. 1967 Jul 29;3(5560):285–286. doi: 10.1136/bmj.3.5560.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baublis J. V., Brown G. C. Specific response of the immunoglobulins to rubella infection. Proc Soc Exp Biol Med. 1968 May;128(1):206–210. doi: 10.3181/00379727-128-32979. [DOI] [PubMed] [Google Scholar]
- Best J. M., Banatvala J. E., Watson D. Serum IgM and IgG responses in postnatally acquired rubella. Lancet. 1969 Jul 12;2(7611):65–68. doi: 10.1016/s0140-6736(69)92386-1. [DOI] [PubMed] [Google Scholar]
- Gupta J. D., Peterson V. J. Use of a new buffer system with formalinized sheep erythrocytes in the rubella hemagglutination-inhibition test. Appl Microbiol. 1971 Apr;21(4):749–750. doi: 10.1128/am.21.4.749-750.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stewart G. L., Parkman P. D., Hopps H. E., Douglas R. D., Hamilton J. P., Meyer H. M., Jr Rubella-virus hemagglutination-inhibition test. N Engl J Med. 1967 Mar 9;276(10):554–557. doi: 10.1056/NEJM196703092761006. [DOI] [PubMed] [Google Scholar]
- Vesikari T., Vaheri A. Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies. Br Med J. 1968 Jan 27;1(5586):221–223. doi: 10.1136/bmj.1.5586.221. [DOI] [PMC free article] [PubMed] [Google Scholar]