Abstract
An analysis of the plasma proteins in the sol phase of sputum was carried out using quantitative cross immunoelectrophoresis. The average concentrations of nine plasma proteins were estimated in the sol phase of sputum specimens from 30 patients with chronic bronchitis and the values were compared with the concentrations of these proteins in saliva and serum specimens from the same group of patients.
The results showed that alpha1 antichymotrypsin and IgA concentrations were higher in the sol phase of sputum than would be expected if their presence were due entirely to passive transudation.
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- Becker W., Rapp W., Schwick H. G., Störiko K. Methoden zur quantitativen Bestimmung von Plasmaproteinen durch Immunpräzipitation. Z Klin Chem Klin Biochem. 1968 May;6(3):113–122. [PubMed] [Google Scholar]
- Brogan T. D., Ryley H. C., Allen L., Hutt H. Relation between sputum sol phase composition and diagnosis in chronic chest diseases. Thorax. 1971 Jul;26(4):418–423. doi: 10.1136/thx.26.4.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clarke H. G., Freeman T. Quantitative immunoelectrophoresis of human serum proteins. Clin Sci. 1968 Oct;35(2):403–413. [PubMed] [Google Scholar]
- FLEISCHMAN J. B., PAIN R. H., PORTER R. R. Reduction of gamma-globulins. Arch Biochem Biophys. 1962 Sep;Suppl 1:174–180. [PubMed] [Google Scholar]
- Falk G. A., Okinaka A. J., Siskind G. W. Immunoglobulins in the bronchial washings of patients with chronic obstructive pulmonary disease. Am Rev Respir Dis. 1972 Jan;105(1):14–21. doi: 10.1164/arrd.1972.105.1.14. [DOI] [PubMed] [Google Scholar]
- Heimburger N., Haupt H. Charakterisierung von alpha-1-X-Glykoprotein als Chymotrypsin-Inhibitor des Humanplasmas. Clin Chim Acta. 1965 Jul;12(1):116–118. doi: 10.1016/0009-8981(65)90118-x. [DOI] [PubMed] [Google Scholar]
- LIEBERMAN J., TRIMMER B. M., KURNICK N. B. SUBSTRATE SPECIFICITY OF PROTEASE ACTIVITIES IN PURULENT SPUTUM. Lab Invest. 1965 Mar;14:249–257. [PubMed] [Google Scholar]
- Lieberman J., Kaneshiro W. Inhibition of leukocytic elastase from purulent sputum by alpha 1 -antitrypsin. J Lab Clin Med. 1972 Jul;80(1):88–101. [PubMed] [Google Scholar]
- Lopez M., Tsu T., Hyslop N. E., Jr Studies of electroimmunodiffusion: immunochemical quantitation of proteins in dilute solutions. Immunochemistry. 1969 Jul;6(4):513–526. doi: 10.1016/0019-2791(69)90191-8. [DOI] [PubMed] [Google Scholar]
- Martinez-Tello F. J., Braun D. G., Blanc W. A. Immunoglobulin production in bronchial mucosa and bronchial lymph nodes, particularly in cystic fibrosis of the pancreas. J Immunol. 1968 Nov;101(5):989–1003. [PubMed] [Google Scholar]
- Ohlsson K. Interactions between bovine -chymotrypsin and the protease inhibitors of human and dog serum in vitro. Scand J Clin Lab Invest. 1971 Sep;28(1):5–11. doi: 10.3109/00365517109090656. [DOI] [PubMed] [Google Scholar]
- Ryler H. C. An immunoelectrophoretic study of the soluble secretory proteins of sputum. Biochim Biophys Acta. 1972 Jul 21;271(2):300–309. [PubMed] [Google Scholar]
- Ryley H. C. An electrophoretic study of the sol phase of sputum. J Lab Clin Med. 1970 Mar;75(3):382–390. [PubMed] [Google Scholar]
- Ryley H. C., Brogan T. D. Variation in the composition of sputum in chronic chest diseases. Br J Exp Pathol. 1968 Dec;49(6):625–633. [PMC free article] [PubMed] [Google Scholar]
- Zschocke R. H., Grieble H. G., Bach G. L., Anderson T. O. Studies on IgA. I. Fractionation procedure for isolation of IgA from pooled normal human plasma. J Immunol. 1969 Mar;102(3):625–637. [PubMed] [Google Scholar]