Skip to main content
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1974 May;27(5):392–398. doi: 10.1136/jcp.27.5.392

The nature of the serum alkaline phosphatases in liver diseases

C P Price 1, H G Sammons 1
PMCID: PMC478132  PMID: 4854965

Abstract

The two main alkaline phosphatase isoenzymes in serum from patients with extrahepatic biliary obstruction have been purified. Properties of these isoenzymes, such as electrophoretic mobility, separation on gel filtration, ultracentrifugation characteristics, Michaelis constants, and sensitivity to neuraminidase have been studied and compared with the isoenzymes of liver and bile. The results show that there is an abnormal isoenzyme in the serum from these patients and that this isoenzyme is similar but not identical with the main bile isoenzyme. It is suggested that it may be associated with a lipoprotein carrier.

Full text

PDF
395

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER R. W., PELLEGRINO C. The separation and detection of serum enzymes by paper electrophoresis. Scand J Clin Lab Invest. 1954;6(2):94–101. doi: 10.3109/00365515409134847. [DOI] [PubMed] [Google Scholar]
  2. CHIANDUSSI L., GREENE S. F., SHERLOCK S. Serum alkaline phosphatase fractions in hepato-biliary and bone diseases. Clin Sci. 1962 Jun;22:425–434. [PubMed] [Google Scholar]
  3. Dunne J., Fennelly J. J., McGeeney K. Separation of alkaline phosphatase enzymes in human serum using gel-filtration (Sephadex G-200) techniques. Cancer. 1967 Jan;20(1):71–76. doi: 10.1002/1097-0142(1967)20:1<71::aid-cncr2820200111>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  4. FLODIN P., KILLANDER J. Fractionation of human-serum proteins by gel filtration. Biochim Biophys Acta. 1962 Oct 8;63:402–410. doi: 10.1016/0006-3002(62)90104-x. [DOI] [PubMed] [Google Scholar]
  5. GORDON S. ALKALINE PHOSPHATASE ISOENZYMES IN SERUM AND TISSUE. S Afr Med J. 1965 Jan 16;39:49–53. [PubMed] [Google Scholar]
  6. HODSON A. W., LATNER A. L., RAINE L. Iso-enzymes of alkaline phosphatase. Clin Chim Acta. 1962 Mar;7:255–261. doi: 10.1016/0009-8981(62)90018-9. [DOI] [PubMed] [Google Scholar]
  7. Hill P. G., Sammons H. G. An interpretation of the elevation of serum alkaline phosphatase in disease. J Clin Pathol. 1967 Jul;20(4):654–659. doi: 10.1136/jcp.20.4.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jennings R. C., Brocklehurst D., Hirst M. A comparative study of alkaline phosphatase enzymes using starch-gel electrophoresis and sephadex gel-filtration with special reference to high molecular weight enzymes. Clin Chim Acta. 1970 Nov;30(2):509–517. doi: 10.1016/0009-8981(70)90144-0. [DOI] [PubMed] [Google Scholar]
  9. KIND P. R., KING E. J. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol. 1954 Nov;7(4):322–326. doi: 10.1136/jcp.7.4.322. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KORNER N. H. Distribution of alkaline phosphatase in serum protein fractions. J Clin Pathol. 1962 May;15:195–199. doi: 10.1136/jcp.15.3.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KOWLESSAR O. D., PERT J. H., HAEFFNER L. J., SLEISENGER M. H. Localization of 5-nucleotidase and non-specific alkaline phosphatase by starch gel electrophoresis. Proc Soc Exp Biol Med. 1959 Jan;100(1):191–193. doi: 10.3181/00379727-100-24568. [DOI] [PubMed] [Google Scholar]
  12. Keiding R. Mechanisms of phosphohydrolase transport and of homeostasis. Ann N Y Acad Sci. 1969 Oct 14;166(2):510–522. doi: 10.1111/j.1749-6632.1969.tb46417.x. [DOI] [PubMed] [Google Scholar]
  13. MOSS D. W., CAMPBELL D. M., ANAGNOSTOU-KAKARAS E., KING E. J. Characterization of tissue alkaline phosphatases and their partial purification by starch-gel electrophoresis. Biochem J. 1961 Nov;81:441–447. doi: 10.1042/bj0810441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MOSS D. W. Iso-enzymes of alkaline phosphatase in autolysed and butanol-extracted liver preparations. Nature. 1962 Mar 10;193:981–982. doi: 10.1038/193981a0. [DOI] [PubMed] [Google Scholar]
  15. Pope C. E., 2nd, Cooperband S. R. Protein characteristics of serum and bile alkaline phosphatase. Gastroenterology. 1966 May;50(5):631–636. [PubMed] [Google Scholar]
  16. Price C. P., Hill P. G., Sammons H. G. The nature of the alkaline phosphatases of bile. J Clin Pathol. 1972 Feb;25(2):149–154. doi: 10.1136/jcp.25.2.149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. ROSENBERG I. N. Zone electrophoretic studies of serum alkaline phosphatase. J Clin Invest. 1959 Apr;38(4):630–644. doi: 10.1172/JCI103841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Smith I., Lightstone P. J., Perry J. D. Separation of human tissue alkaline phosphatases by electrophoresis on acrylamide disc gels. Clin Chim Acta. 1968 Mar;19(3):499–505. doi: 10.1016/0009-8981(68)90278-7. [DOI] [PubMed] [Google Scholar]
  19. TASWELL H. F., JEFFERS D. M. ISOENZYMES OF SERUM ALKALINE PHOSPHATASE IN HEPATOBILIARY AND SKELETAL DISEASE. Am J Clin Pathol. 1963 Oct;40:349–356. doi: 10.1093/ajcp/40.4.349. [DOI] [PubMed] [Google Scholar]
  20. Walker A. W., Pollard A. C. Observations on serum alkaline phosphatase electrophoretic patterns on polyacrylamide gel with particular reference to the effects of butanol extraction. Clin Chim Acta. 1971 Aug;34(1):19–29. doi: 10.1016/0009-8981(71)90062-3. [DOI] [PubMed] [Google Scholar]
  21. Wieme R. J., Demeulenaere L. Enzyme assays in liver disease. J Clin Pathol Suppl (Assoc Clin Pathol) 1970;4:51–59. doi: 10.1136/jcp.s1-4.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES