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. 1993 Nov 15;90(22):10593–10597. doi: 10.1073/pnas.90.22.10593

Genetic analysis of homomeric interactions of human immunodeficiency virus type 1 integrase using the yeast two-hybrid system.

G V Kalpana 1, S P Goff 1
PMCID: PMC47823  PMID: 8248150

Abstract

The retroviral integrase protein (IN) is responsible for catalyzing a concerted integration reaction in which the two termini of linear viral DNA are joined to host DNA. To probe the potential for IN to form protein multimers, we used the yeast two-hybrid system. The coexpression of a GAL4 DNA binding domain-IN fusion and a GAL4 activation domain-IN fusion together resulted in the successful activation of a GAL4-responsive LacZ reporter gene. The system was used to examine a variety of IN deletion mutants. The results suggest that the central region of the protein is necessary for multimerization and that the N-terminal zinc finger region is not important.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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