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. 1964 Jan;17(1):31–38. doi: 10.1136/jcp.17.1.31

The urinary excretion and tissue retention of cyanocobalamin by subjects given repeated parenteral doses

J F Adams 1
PMCID: PMC480666  PMID: 14099998

Abstract

The urinary excretion of injected cyanocobalamin was studied in 30 previously untreated vitamin B12-deficient patients by measuring the urinary radioactivity after repeated injections of 58Co vitamin B12. The dose range used was 54 to 30,000 μg., each patient receiving the same dose at each injection.

The results show that there is no constant trend to the excretion of greater or smaller proportions of the amount injected. It is also shown that there is a wide variation in the amounts excreted by each individual and between individual patients and that the capacity of the tissues to retain injected cyanocobalamin is very great, the amounts retained often being greatly in excess of the normal body stores. The excretion of radioactivity after parenteral infections of 58Co vitamin B12 was usually complete within 24 hours but exceptions to this were seen.

The significance and value of the results is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHOW B. F., ROSENBLUM C., SILBER R. H., WOODBURY D. T., YAMAMOTO R., LAND C. A. Oral administration of vitamin B12 containing cobalt60 to rats. Proc Soc Exp Biol Med. 1951 Feb;76(2):393–395. doi: 10.3181/00379727-76-18501. [DOI] [PubMed] [Google Scholar]
  2. ESTRADA S. C., LANG C. A., CHOW B. F. The application of vitamin B12 tolerance tests to American and Mexican subjects. J Lab Clin Med. 1954 Mar;43(3):406–410. [PubMed] [Google Scholar]
  3. GIRDWOOD R. H. Microbiological methods of assay in clinical medicine with particular reference to the investigation of deficiency of vitamin B12 and folic acid. Scott Med J. 1960 Jan;5:10–22. doi: 10.1177/003693306000500103. [DOI] [PubMed] [Google Scholar]
  4. HARTE R. A., CHOW B. F., BARROWS L. Storage and elimination of vitamin B12 in the rat. J Nutr. 1953 Apr;49(4):669–678. doi: 10.1093/jn/49.4.669. [DOI] [PubMed] [Google Scholar]
  5. KAY A. W. Effect of large doses of histamine on gastric secretion of HCI; an augmented histamine test. Br Med J. 1953 Jul 11;2(4827):77–80. doi: 10.1136/bmj.2.4827.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KING E. J., BAIN D. S. A simplified silver iodate method for the determination of chloride. Biochem J. 1951 Jan;48(1):51–53. doi: 10.1042/bj0480051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MACLEAN L. D., BLOCH H. S. Gastrointestinal absorption and urinary excretion of vitamin B12-Co60. Proc Soc Exp Biol Med. 1954 Oct;87(1):171–174. doi: 10.3181/00379727-87-21324. [DOI] [PubMed] [Google Scholar]
  8. MOLLIN D. L., ROSS G. I. Serum vitamin B12 concentrations of patients with megaloblastic anaemia after treatment with vitamin B12, folic acid, or folinic acid. Br Med J. 1953 Sep 19;2(4837):640–645. doi: 10.1136/bmj.2.4837.640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SCHILLING R. F. Intrinsic factor studies II. The effect of gastric juice on the urinary excretion of radioactivity after the oral administration of radioactive vitamin B12. J Lab Clin Med. 1953 Dec;42(6):860–866. [PubMed] [Google Scholar]
  10. SHINTON N. K. Total serum vitamin B12 concentration in normal human adult serum assayed by Euglena gracilis. Clin Sci. 1959 Aug;18:389–398. [PubMed] [Google Scholar]
  11. SMITH E. L. Radioactive penicillin and vitamin B12. Br Med Bull. 1952;8(2-3):203–205. doi: 10.1093/oxfordjournals.bmb.a074107. [DOI] [PubMed] [Google Scholar]
  12. SOKOLOFF M. F., SANNEMAN E. H., Jr, BEARD M. F. Urinary excretion of vitamin B12. Blood. 1952 Feb;7(2):243–250. [PubMed] [Google Scholar]

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