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. 1992 Mar 15;89(6):2180–2184. doi: 10.1073/pnas.89.6.2180

Common double- and single-stranded DNA binding factor for a sterol regulatory element.

H C Stark 1, O Weinberger 1, J Weinberger 1
PMCID: PMC48620  PMID: 1549579

Abstract

A cis-acting element necessary for sterol regulation, SRE-1, has previously been identified in the promoters of the low density lipoprotein receptor, hydroxymethylglutaryl (HMG)-CoA reductase, and HMG-CoA synthase genes. In this report we describe a nuclear factor, SRE-BF, isolated from Chinese hamster ovary nuclear extracts, that binds to the SRE-1 octanucleotide sequence. In addition to sequence-specific binding to SRE-1, as indicated by competition analysis with double-stranded DNA fragments, single-stranded oligomer DNA sequences also compete for binding in a sequence-specific fashion. Photochemical cross-linking experiments suggest that a common protein factor, with apparent molecular mass of 45-49 kDa, recognizes both single-stranded and double-stranded SRE-1. The binding specificity of SRE-BF to single-stranded SRE-1 closely correlates with the reported in vivo ability of SRE-1 to direct sterol responsiveness of transcription.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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