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. 1992 Apr 1;89(7):2794–2798. doi: 10.1073/pnas.89.7.2794

Single-residue changes in class I major histocompatibility complex molecules stimulate responses to self peptides.

A G Grandea 3rd 1, M J Bevan 1
PMCID: PMC48749  PMID: 1557385

Abstract

Single-residue changes were introduced into the murine major histocompatibility complex class I molecule H-2Kb at positions 65 and 69, which are predicted to point up from the alpha-helix of the alpha 1 domain and not into the peptide binding groove. Mutated and wild-type genes were transfected into the murine cell line P815 (H-2d). We present evidence that the changes did not affect the binding of three foreign peptides that are recognized by cytotoxic T lymphocytes (CTL) in association with H-2Kb. Additionally, the mutants provoked strong alloreactive responses in T cells from mice expressing unmutated H-2Kb. The alloreactive CTL were specific for self peptides, which could be extracted from wild-type H-2Kb molecules, recognized in the context of the mutant class I.

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Selected References

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