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. 1992 May 15;89(10):4663–4667. doi: 10.1073/pnas.89.10.4663

Dodeca satellite: a conserved G+C-rich satellite from the centromeric heterochromatin of Drosophila melanogaster.

J P Abad 1, M Carmena 1, S Baars 1, R D Saunders 1, D M Glover 1, P Ludeña 1, C Sentis 1, C Tyler-Smith 1, A Villasante 1
PMCID: PMC49143  PMID: 1584802

Abstract

To identify sequences from the centromeric region, we have constructed a Drosophila melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA from the minichromosome Dp(1;f)1187 derived from the X chromosome. We describe the structure of one clone isolated in this way. This YAC is structurally unstable and contains tandemly repeated G+C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences are found in the genomes of organisms as distant as Arabidopsis thaliana and Homo sapiens.

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Selected References

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