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. 1992 Jun 1;89(11):5192–5196. doi: 10.1073/pnas.89.11.5192

pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.

M D Schaller 1, C A Borgman 1, B S Cobb 1, R R Vines 1, A B Reynolds 1, J T Parsons 1
PMCID: PMC49256  PMID: 1594631

Abstract

Expression of the Rous sarcoma virus-encoded oncoprotein, pp60v-src, subverts the normal regulation of cell growth, which results in oncogenic transformation. This process requires the intrinsic protein-tyrosine kinase activity of pp60v-src and is associated with an increase in tyrosine phosphorylation of a number of cellular proteins, candidate substrates for pp60v-src. We report here the isolation of a cDNA encoding a protein, pp125, that is a major phosphotyrosine-containing protein in untransformed chicken embryo cells and exhibits an increase in phosphotyrosine in pp60v-src-transformed chicken embryo cells. This cDNA encodes a cytoplasmic protein-tyrosine kinase which, based upon its predicted amino acid sequence and structure, is the prototype for an additional family of protein-tyrosine kinases. Immunofluorescence localization experiments show that pp125 is localized to focal adhesions; hence, we suggest the name focal adhesion kinase.

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Selected References

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