Abstract
Tubercle bacilli, which survived heat fixation, were detected with a slide culture technique which allowed the entire smear to be examined. Both conventional flame fixation and the use of a controlled hot-plate failed to render tuberculous sputum smears safe for further handling. Smears which were stained with the phenol-auramine method failed to yield growth on culture. If delay between preparation and staining is unavoidable, it is recommended that smears are given additional treatment to prevent the survival of tubercle bacilli.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blair E. B., Bretherton W. W., Tull A. H. A method to render unstained mycobacterial smears safe for storage or shipment. Appl Microbiol. 1972 Apr;23(4):826–826. doi: 10.1128/am.23.4.826-826.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clancey J. K., Allen B. W., Rogers D. T., Smith L. S., Aber V., Mitchison D. A. Comparison of machine and manual staining of direct smears for acid-fast bacilli by fluorescence microscopy. J Clin Pathol. 1976 Oct;29(10):931–933. doi: 10.1136/jcp.29.10.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitchison D. A. Standard smears for grading the content of acid-fast bacilli in sputum. Tubercle. 1966 Sep;47(3):289–291. doi: 10.1016/s0041-3879(66)80008-9. [DOI] [PubMed] [Google Scholar]
- STRITCH J. H., DICKINSON J. M. Slide culture estimation of sensitivity of Mycobacterium tuberculosis. Ir J Med Sci. 1958 May;(389):237–240. doi: 10.1007/BF02951554. [DOI] [PubMed] [Google Scholar]
- WALLACE J. G. The heat resistance of tubercle bacilli in the lungs of infected mice. Am Rev Respir Dis. 1961 Jun;83:866–871. doi: 10.1164/arrd.1961.83.6.866. [DOI] [PubMed] [Google Scholar]