Abstract
A sensitive method for detecting rubella IgM antibody, the first step of which is the attachment of the serum IgM to a solid phase, is described. Specific IgM antibody was found in all 52 people with acquired rubella in the early convalescent period, in all 38 infants with congenital rubella examined in the first seven months of life, and in 23 of 26 people immunised one to four months before testing. Twenty-four of 44 rheumatoid factor-positive sera, however, gave false-positive readings. In routine use the test was economical of both reagents and time.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cradock-Watson J. E., Ridehalgh M. K., Pattison J. R., Anderson M. J., Kangro H. O. Comparison of immunofluorescence and radioimmunoassay for detecting IgM antibody in infants with the congenital rubella syndrome. J Hyg (Lond) 1979 Dec;83(3):413–423. doi: 10.1017/s0022172400026243. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Diment J. A., Chantler S. M. Enzyme immunoassay for detection of rubella specific IgM antibody. Lancet. 1981 Feb 14;1(8216):394–395. doi: 10.1016/s0140-6736(81)91723-2. [DOI] [PubMed] [Google Scholar]
- Duermeyer W., Wielaard F., van der Veen J. A new principle for the detection of specific IgM antibodies applied in an ELISA for hepatitis A. J Med Virol. 1979;4(1):25–32. doi: 10.1002/jmv.1890040104. [DOI] [PubMed] [Google Scholar]
- Morgan-Capner P., Davies E., Pattison J. R. Rubella-specific IgM detection using Sephacryl S-300 gel filtration. J Clin Pathol. 1980 Nov;33(11):1082–1085. doi: 10.1136/jcp.33.11.1082. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mortimer P. P., Tedder R. S., Hamblig M. H., Shafi M. S., Burkhardt F., Schilt U. Antibody capture radioimmunoassay for anti-rubella IgM. J Hyg (Lond) 1981 Apr;86(2):139–153. doi: 10.1017/s0022172400068856. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
- Roggendorf M., Frösner G. G., Deinhardt F., Scheid R. Comparison of solid phase test systems for demonstrating antibodies against hepatitis A virus (anti-Hav) of the IgM-class. J Med Virol. 1980;5(1):47–62. doi: 10.1002/jmv.1890050107. [DOI] [PubMed] [Google Scholar]
- Smith A. M., Tedder R. S. Development of an enzyme-linked immunosorbent assay (ELISA) for hepatitis B e antigen and antibody. J Virol Methods. 1981 Jul;3(1):1–11. doi: 10.1016/0166-0934(81)90017-3. [DOI] [PubMed] [Google Scholar]