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. 1992 Apr;45(4):314–318. doi: 10.1136/jcp.45.4.314

Differential effect of chronic hepatitis D virus infection on intrahepatic expression of hepatitis B viral antigen.

J Y Lau 1, B C Portmann 1, G J Alexander 1, R Williams 1
PMCID: PMC495271  PMID: 1577968

Abstract

AIMS: To determine how chronic hepatitis D virus (HDV) infection affects intrahepatic hepatitis B virus (HBV) antigen expression. METHODS: Ninety eight liver biopsy specimens from 68 patients seropositive for total antibody to HDV were studied by immunohistochemistry, and the amount of HBV antigens was also quantified by radioimmunoassay in 12 patients and compared with 30 patients with chronic HBV infection. RESULTS: Forty nine of the 68 patients were positive for intrahepatic HDV antigen and only five were positive for HBV core antigen (HBcAg). HBV surface antigen (HBsAg) was present in 55 (80.9%) patients and was always cytoplasmic in distribution. Hepatic pre-S1 and pre-S2 expressions paralleled that of HBsAg, and were detected in 53 (77.9%) and 54 (79.4%) patients, respectively. There was no relation between the intrahepatic expression of HDV antigen and HBsAg/pre-S1/pre-S2. Follow up biopsy specimens in 25 patients showed either static or deteriorating histology while intrahepatic HDV antigen remained the same or fell. The patients with intrahepatic expression of HBcAg had either absent or noticeably decreased expression of HBcAg in their follow up biopsy specimens (median two years). In contrast, HBsAg/pre-S1/pre-S2 were the same or increased (p less than 0.001). Quantification of intrahepatic HBsAg in patients with chronic HDV infection (0.61 pg/hepatocyte, range: 0.05-1.08, n = 12) showed no difference with patients with chronic HBV infection alone (0.64 pg/hepatocyte, range: 0.02-1.02, n = 30, p = NS). CONCLUSION: These data indicate that chronic HDV infection suppresses intrahepatic expression of HBcAg but not HbsAg and pre-S antigens, suggesting a differential effect of chronic HDV infection on HBV gene expression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonino F., Heermann K. H., Rizzetto M., Gerlich W. H. Hepatitis delta virus: protein composition of delta antigen and its hepatitis B virus-derived envelope. J Virol. 1986 Jun;58(3):945–950. doi: 10.1128/jvi.58.3.945-950.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cattaneo R., Will H., Schaller H. Hepatitis B virus transcription in the infected liver. EMBO J. 1984 Sep;3(9):2191–2196. doi: 10.1002/j.1460-2075.1984.tb02113.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chisari F. V., Filippi P., Buras J., McLachlan A., Popper H., Pinkert C. A., Palmiter R. D., Brinster R. L. Structural and pathological effects of synthesis of hepatitis B virus large envelope polypeptide in transgenic mice. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6909–6913. doi: 10.1073/pnas.84.19.6909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chu C. M., Liaw Y. F. Intrahepatic distribution of hepatitis B surface and core antigens in chronic hepatitis B virus infection. Hepatocyte with cytoplasmic/membranous hepatitis B core antigen as a possible target for immune hepatocytolysis. Gastroenterology. 1987 Jan;92(1):220–225. doi: 10.1016/0016-5085(87)90863-8. [DOI] [PubMed] [Google Scholar]
  5. Chu C. M., Liaw Y. F. Studies on the composition of the mononuclear cell infiltrates in liver from patients with chronic active delta hepatitis. Hepatology. 1989 Dec;10(6):911–915. doi: 10.1002/hep.1840100603. [DOI] [PubMed] [Google Scholar]
  6. Davies S. E., Portmann B. C., O'Grady J. G., Aldis P. M., Chaggar K., Alexander G. J., Williams R. Hepatic histological findings after transplantation for chronic hepatitis B virus infection, including a unique pattern of fibrosing cholestatic hepatitis. Hepatology. 1991 Jan;13(1):150–157. [PubMed] [Google Scholar]
  7. Fagan E. A., Guarner P., Perera S. D., Trowbridge R., Rolando N., Davison F., Williams R. Quantitation of hepatitis B virus DNA (HBV DNA) in serum using the spot hybridization technique and scintillation counting. J Virol Methods. 1985 Dec;12(3-4):251–262. doi: 10.1016/0166-0934(85)90136-3. [DOI] [PubMed] [Google Scholar]
  8. Gowans E. J., Burrell C. J. Widespread presence of cytoplasmic HBcAg in hepatitis B infected liver detected by improved immunochemical methods. J Clin Pathol. 1985 Apr;38(4):393–398. doi: 10.1136/jcp.38.4.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grendele M., Colledan M., Gridelli B., Rossi G., Fassati L. R., Ferla G., Maggi U., Reggiani P., Doglia M., Lunghi G. Does the experience with liver transplantation suggest that hepatitis delta virus is not cytopathic? Transplant Proc. 1990 Aug;22(4):1551–1553. [PubMed] [Google Scholar]
  10. Hadzic N., Alberti A., Portmann B., Vergani D. Detection of hepatitis B virus pre-S1 and pre-S2 determinants in paraffin wax embedded liver tissue: importance of reagents used. J Clin Pathol. 1991 Jul;44(7):554–557. doi: 10.1136/jcp.44.7.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Krogsgaard K., Aldershvile J., Kryger P., Andersson P., Nielsen J. O., Hansson B. G. Hepatitis B virus DNA, HBeAg and delta infection during the course from acute to chronic hepatitis B virus infection. Hepatology. 1985 Sep-Oct;5(5):778–782. doi: 10.1002/hep.1840050512. [DOI] [PubMed] [Google Scholar]
  12. Lau J. Y., Bain V. G., Davies S. E., Alexander G. J., Williams R. Export of intracellular HBsAg in chronic hepatitis B virus infection is related to viral replication. Hepatology. 1991 Sep;14(3):416–421. [PubMed] [Google Scholar]
  13. Lau J. Y., Hansen L. J., Bain V. G., Chaggar K., Smith H. M., Portman B. C., Vergani D., Alexander G. J., Williams R. Expression of intrahepatic hepatitis D viral antigen in chronic hepatitis D virus infection. J Clin Pathol. 1991 Jul;44(7):549–553. doi: 10.1136/jcp.44.7.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Michel M. L., Tiollais P. Structure and expression of the hepatitis B virus genome. Hepatology. 1987 Jan-Feb;7(1 Suppl):61S–63S. doi: 10.1002/hep.1840070711. [DOI] [PubMed] [Google Scholar]
  15. Miller R. H., Kaneko S., Chung C. T., Girones R., Purcell R. H. Compact organization of the hepatitis B virus genome. Hepatology. 1989 Feb;9(2):322–327. doi: 10.1002/hep.1840090226. [DOI] [PubMed] [Google Scholar]
  16. Rizzetto M., Canese M. G., Gerin J. L., London W. T., Sly D. L., Purcell R. H. Transmission of the hepatitis B virus-associated delta antigen to chimpanzees. J Infect Dis. 1980 May;141(5):590–602. doi: 10.1093/infdis/141.5.590. [DOI] [PubMed] [Google Scholar]
  17. Rizzetto M., Canese M. G., Gerin J. L., London W. T., Sly D. L., Purcell R. H. Transmission of the hepatitis B virus-associated delta antigen to chimpanzees. J Infect Dis. 1980 May;141(5):590–602. doi: 10.1093/infdis/141.5.590. [DOI] [PubMed] [Google Scholar]
  18. Rizzetto M. Hepatitis delta: the virus and the disease. J Hepatol. 1990;11 (Suppl 1):S145–S148. doi: 10.1016/0168-8278(90)90183-r. [DOI] [PubMed] [Google Scholar]
  19. Rizzetto M., Verme G. Delta hepatitis--present status. J Hepatol. 1985;1(2):187–193. doi: 10.1016/s0168-8278(85)80766-2. [DOI] [PubMed] [Google Scholar]
  20. Trevisan A., Gudat F., Busachi C., Stöcklin E., Bianchi L. An improved method for HBcAg demonstration in paraffin-embedded liver tissue. Liver. 1982 Dec;2(4):331–339. doi: 10.1111/j.1600-0676.1982.tb00831.x. [DOI] [PubMed] [Google Scholar]
  21. Trevisan A., Gudat F., Busachi C., Stöcklin E., Bianchi L. An improved method for HBcAg demonstration in paraffin-embedded liver tissue. Liver. 1982 Dec;2(4):331–339. doi: 10.1111/j.1600-0676.1982.tb00831.x. [DOI] [PubMed] [Google Scholar]

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