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. 1992 Feb;45(2):143–148. doi: 10.1136/jcp.45.2.143

Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours.

I Kuzu 1, R Bicknell 1, A L Harris 1, M Jones 1, K C Gatter 1, D Y Mason 1
PMCID: PMC495659  PMID: 1371777

Abstract

AIMS: To determine the distribution of factor VIII related antigen, CD31, CD34 and CD36 in normal and malignant human vascular tissues using a panel of well characterised monoclonal antibodies. METHODS: Frozen and fixed material from a wide range of normal tissues and routinely processed material from 43 benign and malignant vascular tumours were examined. Single immunocytochemical labelling was performed using the APAAP technique. Double staining involved the sequential use of APAAP with the peroxidase method. RESULTS: Human vascular endothelium was antigenically heterogeneous. One of the most restricted markers was factor VIII related antigen, despite its having been widely used in diagnostic pathology as a marker of vascular endothelium and of the tumours which arise from it. Three antibodies against factor VIII related antigen, CD31 (JC70) and CD34 (QBend 10) were identified as immunostaining routinely processed, formalin fixed, paraffin wax sections. Each antibody gave different staining when tested on a range of vascular tumours, both benign and malignant. CONCLUSIONS: A small panel of three reagents (factor VIII related antigen, CD31 (JC70) and CD34 (QBend 10)) should be used by diagnostic pathologists who want to show the presence of cells of endothelial origin in routine material.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Battifora H. The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. Lab Invest. 1986 Aug;55(2):244–248. [PubMed] [Google Scholar]
  2. Böhling T., Paetau A., Ekblom P., Haltia M. Distribution of endothelial and basement membrane markers in angiogenic tumors of the nervous system. Acta Neuropathol. 1983;62(1-2):67–72. doi: 10.1007/BF00684922. [DOI] [PubMed] [Google Scholar]
  3. Fajardo L. F. The complexity of endothelial cells. A review. Am J Clin Pathol. 1989 Aug;92(2):241–250. doi: 10.1093/ajcp/92.2.241. [DOI] [PubMed] [Google Scholar]
  4. Fawcett J., Harris A. L., Bicknell R. Isolation and properties in culture of human adrenal capillary endothelial cells. Biochem Biophys Res Commun. 1991 Jan 31;174(2):903–908. doi: 10.1016/0006-291x(91)91503-5. [DOI] [PubMed] [Google Scholar]
  5. Fina L., Molgaard H. V., Robertson D., Bradley N. J., Monaghan P., Delia D., Sutherland D. R., Baker M. A., Greaves M. F. Expression of the CD34 gene in vascular endothelial cells. Blood. 1990 Jun 15;75(12):2417–2426. [PubMed] [Google Scholar]
  6. Fletcher C. D., Ramani P. QBEnd/10: a useful, but by no means specific, marker of Kaposi's sarcoma. J Pathol. 1990 Nov;162(3):273–274. doi: 10.1002/path.1711620315. [DOI] [PubMed] [Google Scholar]
  7. Jefferies W. A., Brandon M. R., Hunt S. V., Williams A. F., Gatter K. C., Mason D. Y. Transferrin receptor on endothelium of brain capillaries. Nature. 1984 Nov 8;312(5990):162–163. doi: 10.1038/312162a0. [DOI] [PubMed] [Google Scholar]
  8. Kagawa H., Fujimoto S. Electron-microscopic and immunocytochemical analyses of Weibel-Palade bodies in the human umbilical vein during pregnancy. Cell Tissue Res. 1987 Sep;249(3):557–563. doi: 10.1007/BF00217327. [DOI] [PubMed] [Google Scholar]
  9. Kinjo T., Takashi M., Miyake K., Nagura H. Phenotypic heterogeneity of vascular endothelial cells in the human kidney. Cell Tissue Res. 1989 Apr;256(1):27–34. doi: 10.1007/BF00224715. [DOI] [PubMed] [Google Scholar]
  10. Koo C. H., Mason D. Y., Miller R., Ben-Ezra J., Sheibani K., Rappaport H. Additional evidence that "plasmacytoid T-cell lymphoma" associated with chronic myeloproliferative disorders is of macrophage/monocyte origin. Am J Clin Pathol. 1990 Jun;93(6):822–827. doi: 10.1093/ajcp/93.6.822. [DOI] [PubMed] [Google Scholar]
  11. Lenzi R., Alpini G., Liu M. H., Rand J. H., Tavoloni N. von Willebrand factor antigen is not an accurate marker of rat and guinea pig liver endothelial cells. Liver. 1990 Dec;10(6):372–379. doi: 10.1111/j.1600-0676.1990.tb00483.x. [DOI] [PubMed] [Google Scholar]
  12. Naiem M., Gerdes J., Abdulaziz Z., Sunderland C. A., Allington M. J., Stein H., Mason D. Y. The value of immunohistological screening in the production of monoclonal antibodies. J Immunol Methods. 1982;50(2):145–160. doi: 10.1016/0022-1759(82)90221-6. [DOI] [PubMed] [Google Scholar]
  13. Newman P. J., Berndt M. C., Gorski J., White G. C., 2nd, Lyman S., Paddock C., Muller W. A. PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science. 1990 Mar 9;247(4947):1219–1222. doi: 10.1126/science.1690453. [DOI] [PubMed] [Google Scholar]
  14. Parums D. V., Cordell J. L., Micklem K., Heryet A. R., Gatter K. C., Mason D. Y. JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J Clin Pathol. 1990 Sep;43(9):752–757. doi: 10.1136/jcp.43.9.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ramani P., Bradley N. J., Fletcher C. D. QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections. Histopathology. 1990 Sep;17(3):237–242. doi: 10.1111/j.1365-2559.1990.tb00713.x. [DOI] [PubMed] [Google Scholar]
  16. Sankey E. A., More L., Dhillon A. P. QBEnd/10: a new immunostain for the routine diagnosis of Kaposi's sarcoma. J Pathol. 1990 Jul;161(3):267–271. doi: 10.1002/path.1711610315. [DOI] [PubMed] [Google Scholar]
  17. Schlingemann R. O., Rietveld F. J., de Waal R. M., Bradley N. J., Skene A. I., Davies A. J., Greaves M. F., Denekamp J., Ruiter D. J. Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma. Lab Invest. 1990 Jun;62(6):690–696. [PubMed] [Google Scholar]
  18. Tannenbaum S. H., Rick M. E., Shafer B., Gralnick H. R. Subendothelial matrix of cultured endothelial cells contains fully processed high molecular weight von Willebrand factor. J Lab Clin Med. 1989 Mar;113(3):372–378. [PubMed] [Google Scholar]
  19. Watt S. M., Karhi K., Gatter K., Furley A. J., Katz F. E., Healy L. E., Altass L. J., Bradley N. J., Sutherland D. R., Levinsky R. Distribution and epitope analysis of the cell membrane glycoprotein (HPCA-1) associated with human hemopoietic progenitor cells. Leukemia. 1987 May;1(5):417–426. [PubMed] [Google Scholar]
  20. Yasunaga C., Sueishi K., Ohgami H., Suita S., Kawanami T. Heterogenous expression of endothelial cell markers in infantile hemangioendothelioma. Immunohistochemical study of two solitary cases and one multiple one. Am J Clin Pathol. 1989 Jun;91(6):673–681. doi: 10.1093/ajcp/91.6.673. [DOI] [PubMed] [Google Scholar]
  21. Zhu P. D., Gu Z. Observation of the activity of factor VIII in the endometrium of women with regular menstrual cycles. Hum Reprod. 1988 Apr;3(3):273–275. doi: 10.1093/oxfordjournals.humrep.a136694. [DOI] [PubMed] [Google Scholar]

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