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. 1982 Oct;35(10):1134–1137. doi: 10.1136/jcp.35.10.1134

Rapid enzyme-linked immunosorbent assay (ELISA) for Aspergillus fumigatus antibodies.

M D Richardson, J M Stubbins, D W Warnock
PMCID: PMC497897  PMID: 6813358

Abstract

A rapid enzyme-linked immunosorbent assay (ELISA) where component incubation periods were shortened to one hour, was compared with agar gel double diffusion (AGDD) and a standard ELISA procedure for detecting antibodies to Aspergillus fumigatus in 28 asthmatic patients with suspected allergic aspergillosis. Using two A fumigatus antigens the rapid ELISA compared well with AGDD and the standard ELISA method. Eleven sera that reacted with both antigens in AGDD were all positive against antigen 1 in both forms of ELISA, but two failed to react with antigen 2 in the standard ELISA and three failed to react with this antigen in the rapid method. Thirteen AGDD-negative sera were also negative in both forms of ELISA. The rapid ELISA provides a sensitive and reproducible test for routine serological investigation of allergic aspergillosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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