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. 1984 Nov;37(11):1227–1234. doi: 10.1136/jcp.37.11.1227

Histometric study of the localisation of lymphocyte subsets and accessory cells in human Mantoux reactions.

J H Gibbs, J Ferguson, R A Brown, K J Kenicer, R C Potts, G Coghill, J Swanson Beck
PMCID: PMC498988  PMID: 6150048

Abstract

Intradermal injection of purified protein derivative produced typical delayed type hypersensitivity reactions in five healthy human subjects. The major subpopulations of lymphocytes and certain accessory cells were located in frozen sections of biopsies of the lesions with monoclonal antibodies and immunohistochemical staining. The densities (expressed as number/unit area for comparison) of the different types of cells were counted at various microanatomical locations in the tissue. The inflammatory cells were concentrated in narrow zones, initially (24 h) only surrounding small blood vessels but later (48-96 h) also around sweat ducts. Lymphocytes were the predominant cell type at these sites with T4 and T8 cells randomly intermixed at a ratio similar to that in the mononuclear cell fraction of the peripheral blood samples removed at the time of biopsy. There was also a scanty diffuse infiltrate in the intervening dermis, but here the T4:T8 ratio was significantly lower than that in the peripheral blood or perivascular cuffs. There was considerable intersubject variation in the relative preponderance of T8 cells in the diffuse infiltrate. The results suggest that there is no subset selection in the initial emigration of lymphocytes through vascular endothelium in the delayed hypersensitivity reaction, but that the subsets behave differently during the subsequent migration through the tissues. It remains to be determined whether the extent to which T8 cells migrate more rapidly than T4 cells through the tissues may influence the reaction at the site of entry of organisms or antigens into the body by altering the balance of the immunoregulatory lymphocyte subsets. This may underlie some of the differences in susceptibility to infection between subjects and determine the type of granuloma that develops in a particular patient.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  2. Center D. M., Cruikshank W. Modulation of lymphocyte migration by human lymphokines. I. Identification and characterization of chemoattractant activity for lymphocytes from mitogen-stimulated mononuclear cells. J Immunol. 1982 Jun;128(6):2563–2568. [PubMed] [Google Scholar]
  3. Cohen S., Ward P. A., Yoshida T., Burek C. L. Biologic activity of extracts of delayed hypersensitivity skin reaction sites. Cell Immunol. 1973 Dec;9(3):363–376. doi: 10.1016/0008-8749(73)90051-8. [DOI] [PubMed] [Google Scholar]
  4. Cruikshank W., Center D. M. Modulation of lymphocyte migration by human lymphokines. II. Purification of a lymphotactic factor (LCF). J Immunol. 1982 Jun;128(6):2569–2574. [PubMed] [Google Scholar]
  5. Duke O., Panayi G. S., Janossy G., Poulter L. W. An immunohistological analysis of lymphocyte subpopulations and their microenvironment in the synovial membranes of patients with rheumatoid arthritis using monoclonal antibodies. Clin Exp Immunol. 1982 Jul;49(1):22–30. [PMC free article] [PubMed] [Google Scholar]
  6. Houck J. C., Chang C. M. The purification and characterization of a lymphokine chemotactic for lymphocytes--lymphotactin. Inflammation. 1977 Jun;2(2):105–113. doi: 10.1007/BF00918672. [DOI] [PubMed] [Google Scholar]
  7. Kardjito T., Grange J. M. Diagnosis of active tuberculosis by immunological methods. 2. Qualitative differences in the dermal response to tuberculin in patients with active pulmonary disease and healthy tuberculin-positive individuals. Tubercle. 1982 Dec;63(4):275–278. doi: 10.1016/s0041-3879(82)80015-9. [DOI] [PubMed] [Google Scholar]
  8. Kardjito T., Grange J. M. Immunological and clinical features of smear-positive pulmonary tuberculosis in East Java. Tubercle. 1980 Dec;61(4):231–238. doi: 10.1016/0041-3879(80)90043-4. [DOI] [PubMed] [Google Scholar]
  9. Modlin R. L., Gebhard J. F., Taylor C. R., Rea T. H. In situ characterization of T lymphocyte subsets in the reactional states of leprosy. Clin Exp Immunol. 1983 Jul;53(1):17–24. [PMC free article] [PubMed] [Google Scholar]
  10. Modlin R. L., Hofman F. M., Meyer P. R., Sharma O. P., Taylor C. R., Rea T. H. In situ demonstration of T lymphocyte subsets in granulomatous inflammation: leprosy, rhinoscleroma and sarcoidosis. Clin Exp Immunol. 1983 Mar;51(3):430–438. [PMC free article] [PubMed] [Google Scholar]
  11. Narayanan R. B., Bhutani L. K., Sharma A. K., Nath I. T cell subsets in leprosy lesions: in situ characterization using monoclonal antibodies. Clin Exp Immunol. 1983 Mar;51(3):421–429. [PMC free article] [PubMed] [Google Scholar]
  12. O'Neill G. J., Parrott D. M. Locomotion of human lymphoid cells. I. Effect of culture and con A on T and non-T lymphocytes. Cell Immunol. 1977 Oct;33(2):257–267. doi: 10.1016/0008-8749(77)90156-3. [DOI] [PubMed] [Google Scholar]
  13. Parrott D. M., Good R. A., O'Neill G. J., Gupta S. Heterogeneity of locomotion in human T cell subsets. Proc Natl Acad Sci U S A. 1978 May;75(5):2392–2395. doi: 10.1073/pnas.75.5.2392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Platt J. L., Grant B. W., Eddy A. A., Michael A. F. Immune cell populations in cutaneous delayed-type hypersensitivity. J Exp Med. 1983 Oct 1;158(4):1227–1242. doi: 10.1084/jem.158.4.1227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Poulter L. W., Seymour G. J., Duke O., Janossy G., Panayi G. Immunohistological analysis of delayed-type hypersensitivity in man. Cell Immunol. 1982 Dec;74(2):358–369. doi: 10.1016/0008-8749(82)90036-3. [DOI] [PubMed] [Google Scholar]
  16. Reinherz E. L., Schlossman S. F. The differentiation and function of human T lymphocytes. Cell. 1980 Apr;19(4):821–827. doi: 10.1016/0092-8674(80)90072-0. [DOI] [PubMed] [Google Scholar]
  17. Roberts C., Potts R. C., Brown R. A., Gibbs J. H., Browning M. C., Beck J. S. The sensitivity of peripheral blood lymphocytes to growth inhibition by hydrocortisone is not determined by their OKT4:OKT8 ratio. Immunol Lett. 1983 Apr;6(4):227–230. doi: 10.1016/0165-2478(83)90009-3. [DOI] [PubMed] [Google Scholar]
  18. Scheynius A., Klareskog L., Forsum U. In situ identification of T lymphocyte subsets and HLA-DR expressing cells in the human skin tuberculin reaction. Clin Exp Immunol. 1982 Aug;49(2):325–330. [PMC free article] [PubMed] [Google Scholar]
  19. Van Voorhis W. C., Kaplan G., Sarno E. N., Horwitz M. A., Steinman R. M., Levis W. R., Nogueira N., Hair L. S., Gattass C. R., Arrick B. A. The cutaneous infiltrates of leprosy: cellular characteristics and the predominant T-cell phenotypes. N Engl J Med. 1982 Dec 23;307(26):1593–1597. doi: 10.1056/NEJM198212233072601. [DOI] [PubMed] [Google Scholar]
  20. Ward P. A., Unanue E. R., Goralnick S. J., Schreiner G. F. Chemotaxis of rat lymphocytes. J Immunol. 1977 Aug;119(2):416–421. [PubMed] [Google Scholar]

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