Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1984 Dec;37(12):1336–1338. doi: 10.1136/jcp.37.12.1336

Cobalamin and folate binding proteins in human tumour tissue.

K Sheppard, D A Bradbury, J M Davies, D R Ryrie
PMCID: PMC499010  PMID: 6096403

Abstract

The serum of an 84 year old man with disseminated carcinoma was found to contain extremely high concentrations of cobalamin and of a cobalamin binding protein with trans-cobalamin I characteristics. Tumour tissue samples obtained at necropsy contained considerably higher concentrations of cobalamin binding protein (R-binder) than normal tissues. Tumour tissues also contained increased concentrations of specific folate binding protein. In all tissues studied a close correlation existed between unsaturated cobalamin and unsaturated folate binding and between total cobalamin and total folate binding. These results suggest related mechanisms for the synthesis of cobalamin binding proteins of the R-binder class and folate binding proteins by tumour tissue.

Full text

PDF
1337

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloomfield F. J., Scott J. M. Identification of a new vitamin B 12 binder (transcobalamin 3) in normal human serum. Br J Haematol. 1972 Jan;22(1):33–42. doi: 10.1111/j.1365-2141.1972.tb08784.x. [DOI] [PubMed] [Google Scholar]
  2. Bradbury D., Sheppard K., Davies J. Specific folate-binding proteins present in some commercial serum albumin preparations. Med Lab Sci. 1983 Apr;40(2):187–188. [PubMed] [Google Scholar]
  3. Burger R. L., Schneider R. J., Mehlman C. S., Allen R. H. Human plasma R-type vitamin B12-binding proteins. II. The role of transcobalamin I, transcobalamin III, and the normal granulocyte vitamin B12-binding protein in the plasma transport of vitamin B12. J Biol Chem. 1975 Oct 10;250(19):7707–7713. [PubMed] [Google Scholar]
  4. Burger R. L., Waxman S., Gilbert H. S., Mehlman C. S., Allen R. H. Isolation and characterization of a novel vitamin B12-binding protein associated with hepatocellular carcinoma. J Clin Invest. 1975 Nov;56(5):1262–1270. doi: 10.1172/JCI108202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carmel R., Eisenberg L. Serum vitamin B12 and transcobalamin abnormalities in patients with cancer. Cancer. 1977 Sep;40(3):1348–1353. doi: 10.1002/1097-0142(197709)40:3<1348::aid-cncr2820400352>3.0.co;2-q. [DOI] [PubMed] [Google Scholar]
  6. Carmel R. Extreme elevation of serum transcobalamin I in patients with metastatic cancer. N Engl J Med. 1975 Feb 6;292(6):282–284. doi: 10.1056/NEJM197502062920603. [DOI] [PubMed] [Google Scholar]
  7. Corrocher R., De Sandre G., Ambrosetti A., Pachor M. L., Bambara L. M., Hoffbrand A. V. Specific and non-specific folate binding protein in normal and malignant human tissues. J Clin Pathol. 1978 Jul;31(7):659–665. doi: 10.1136/jcp.31.7.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eichner E. R., McDonald C. R., Dickson V. L. Elevated serum levels of unsaturated folate binding protein: clinical correlates in a general hospital population. Am J Clin Nutr. 1978 Nov;31(11):1988–1992. doi: 10.1093/ajcn/31.11.1988. [DOI] [PubMed] [Google Scholar]
  9. Fernandes-Costa F., Metz J. Role of serum folate binders in the delivery of folate to tissues and to the fetus. Br J Haematol. 1979 Mar;41(3):335–342. doi: 10.1111/j.1365-2141.1979.tb05866.x. [DOI] [PubMed] [Google Scholar]
  10. Finkler A. E., Green P. D., Hall C. A. Immunological properties of human vitamin B 12 binders. Biochim Biophys Acta. 1970 Jan 20;200(1):151–159. doi: 10.1016/0005-2795(70)90053-x. [DOI] [PubMed] [Google Scholar]
  11. Fischer C. D., Da Costa M., Rothenberg S. P. Properties of purified folate-binding proteins from chronic myelogenous leukemia cells. Biochim Biophys Acta. 1978 Oct 18;543(3):328–339. doi: 10.1016/0304-4165(78)90050-8. [DOI] [PubMed] [Google Scholar]
  12. GOTTLIEBLAU K. S., WASSERMAN L. R., HERBERT V. RAPID CHARCOAL ASSAY FOR INTRINSIC FACTOR (IF), GASTRIC JUICE UNSATURATED B12 BINDING CAPACITY, ANTIBODY TO IF, AND SERUM UNSATURATED B12 BINDING CAPACITY. Blood. 1965 Jun;25:875–884. [PubMed] [Google Scholar]
  13. Hurlimann J., Zuber C. Vitamin B12-binders in human body fluids. I. Antigenic and physico-chemical characteristics. Clin Exp Immunol. 1969 Jan;4(1):125–140. [PMC free article] [PubMed] [Google Scholar]
  14. Kane S. P., Peters T. J. Analytical subcellular fractionation of human granulocytes with reference to the localization of vitamin B12-binding proteins. Clin Sci Mol Med. 1975 Aug;49(2):171–182. doi: 10.1042/cs0490171. [DOI] [PubMed] [Google Scholar]
  15. Ohnishi S. T., Barr J. K. A simplified method of quantitating protein using the biuret and phenol reagents. Anal Biochem. 1978 May;86(1):193–200. doi: 10.1016/0003-2697(78)90334-2. [DOI] [PubMed] [Google Scholar]
  16. Paradinas F. J., Melia W. M., Wilkinson M. L., Portmann B., Johnson P. J., Murray-Lyon I. M., Williams R. High serum vitamin B12 binding capacity as a marker of the fibrolamellar variant of hepatocellular carcinoma. Br Med J (Clin Res Ed) 1982 Sep 25;285(6345):840–842. doi: 10.1136/bmj.285.6345.840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rachmilewitz B., Rachmilewitz M., Chaouat M., Schlesinger M. The synthesis of transcobalamin II, a vitamin B12 transport protein, by stimulated mouse peritoneal macrophages. Biomedicine. 1977 Jul;27(6):213–214. [PubMed] [Google Scholar]
  18. Sheppard K., Ryrie D. Changes in serum levels of cobalamin and cobalamin analogues in folate deficiency. Scand J Haematol. 1980 Nov;25(5):401–406. doi: 10.1111/j.1600-0609.1981.tb01420.x. [DOI] [PubMed] [Google Scholar]
  19. Stenman U. H. Characterization of R-type vitamin B12-binding proteins by isoelectric focusing. I. The relationship between transcobalamin I, transcobalamin III and the granulocyte R protein. Scand J Haematol. 1974;13(2):129–134. doi: 10.1111/j.1600-0609.1974.tb00246.x. [DOI] [PubMed] [Google Scholar]
  20. Stenman U. H. Characterization of R-type vitamin B12-binding proteins by isoelectric focusing. II. Comparison of cobalophilin (r proteins) from different sources. Scand J Clin Lab Invest. 1975 Mar;35(2):147–155. doi: 10.1080/00365517509087218. [DOI] [PubMed] [Google Scholar]
  21. Waxman S., Schreiber C. Measurement of serum folate levels and serum folic acid-binding protein by 3H-PGA radioassay. Blood. 1973 Aug;42(2):281–290. [PubMed] [Google Scholar]
  22. Zittoun J., Zittoun R., Marquet J., Sultan C. The three transcobalamins in myeloproliferative disorders and acute leukaemia. Br J Haematol. 1975 Nov;31(3):287–298. doi: 10.1111/j.1365-2141.1975.tb00860.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES