Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1985 Jan;38(1):18–29. doi: 10.1136/jcp.38.1.18

An immunoperoxidase study of epithelial marker antigens in ulcerative colitis with dysplasia and carcinoma.

D C Allen, J D Biggart, J C Orchin, H Foster
PMCID: PMC499067  PMID: 3918077

Abstract

An immunoperoxidase technique was applied to formalin and Helly fixed paraffin wax sections from cases of ulcerative colitis complicated by dysplasia and carcinoma for carcinoembryonic antigen and components of the colonic secretory immunoglobulin system--namely, secretory component, IgA, and J chain. Sections from both resection specimens and mucosal biopsies were available. Intensity of immunostaining was assessed qualitatively. There was appreciable variation in expression of carcinoembryonic antigen and secretory component antigens. Carcinoembryonic antigen stained heavily in dysplasia and carcinoma while these tissues showed only focal light staining for secretory component. Normal tissue stained heavily for secretory component. The variation in staining intensity for both carcinoembryonic antigen and secretory component in inflamed and regenerative mucosa precluded their use as a reliable diagnostic aid in discriminating these tissues from true dysplasia. Loss of secretory component production or transport or both may be incurred during malignant change, but it should not be assessed as an isolated index of epithelial maturity. The relation with mucosal plasma cells warrants further study to determine more fully the factors affecting tissue secretory component expression.

Full text

PDF
27

Images in this article

19Image
on p.19
20Image
on p.20
21Image
on p.21
22Image
on p.22
23Image
on p.23
24Image
on p.24
25Image
on p.25
25Image
on p.25
26Image
on p.26
26Image
on p.26
27Image
on p.27

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albores-Saavedra J., Nadji M., Morales A. R., Henson D. E. Carcinoembryonic antigen in normal, preneoplastic and neoplastic gallbladder epithelium. Cancer. 1983 Sep 15;52(6):1069–1072. doi: 10.1002/1097-0142(19830915)52:6<1069::aid-cncr2820520623>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  2. Bychkov V., Rothman M., Bardawil W. A. Immunocytochemical localization of carcinoembryonic antigen (CEA), alpha-fetoprotein (AFP), and human chorionic gonadotropin (HCG) in cervical neoplasia. Am J Clin Pathol. 1983 Apr;79(4):414–420. doi: 10.1093/ajcp/79.4.414. [DOI] [PubMed] [Google Scholar]
  3. Ehsanullah M., Filipe M. I., Gazzard B. Mucin secretion in inflammatory bowel disease: correlation with disease activity and dysplasia. Gut. 1982 Jun;23(6):485–489. doi: 10.1136/gut.23.6.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Franzin G., Grigioni W. F., Dina R., Scarpa A., Zamboni G. Mucin secretion and morphological changes of the mucosa in non-neoplastic diseases of the colon. Histopathology. 1983 Sep;7(5):707–718. doi: 10.1111/j.1365-2559.1983.tb02283.x. [DOI] [PubMed] [Google Scholar]
  5. GOLD P., FREEDMAN S. O. DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES. J Exp Med. 1965 Mar 1;121:439–462. doi: 10.1084/jem.121.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldenberg D. M., Sharkey R. M., Primus F. J. Immunocytochemical detection of carcinoembryonic antigen in conventional histopathology specimens. Cancer. 1978 Sep;42(3 Suppl):1546–1553. doi: 10.1002/1097-0142(197809)42:3+<1546::aid-cncr2820420829>3.0.co;2-d. [DOI] [PubMed] [Google Scholar]
  7. Hammarberg C., Slezak P., Tribukait B. Early detection of malignancy in ulcerative colitis. A flow-cytometric DNA study. Cancer. 1984 Jan 15;53(2):291–295. doi: 10.1002/1097-0142(19840115)53:2<291::aid-cncr2820530218>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  8. Isaacson P. Immunoperoxidase study of the secretory immunoglobulin system in colonic neoplasia. J Clin Pathol. 1982 Jan;35(1):14–25. doi: 10.1136/jcp.35.1.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Isaacson P., Judd M. A. Immunohistochemistry of carcinoembryonic antigen in the small intestine. Cancer. 1978 Sep;42(3 Suppl):1554–1559. doi: 10.1002/1097-0142(197809)42:3+<1554::aid-cncr2820420830>3.0.co;2-7. [DOI] [PubMed] [Google Scholar]
  10. Isaacson P., Judd M. A. Immunohistochemistry of carcinoembryonic antigen: characterisation of cross-reactions with other glycoproteins. Gut. 1977 Oct;18(10):779–785. doi: 10.1136/gut.18.10.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Isaacson P., Le Vann H. P. The demonstration of carcinoembryonic antigen in colorectal carcinoma and colonic polyps using an immunoperoxidase technique. Cancer. 1976 Sep;38(3):1348–1356. doi: 10.1002/1097-0142(197609)38:3<1348::aid-cncr2820380339>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  12. Isaacson P. Tissue demonstration of carcinoembryonic antigen (CEA) in ulcerative colitis. Gut. 1976 Jul;17(7):561–567. doi: 10.1136/gut.17.7.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lehy T., Mignon M., Abitbol J. L. Epithelial cell proliferation in the rectal stump of patients with ileorectal anastomosis for ulcerative colitis. Gut. 1983 Nov;24(11):1048–1056. doi: 10.1136/gut.24.11.1048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morson B. C., Pang L. S. Rectal biopsy as an aid to cancer control in ulcerative colitis. Gut. 1967 Oct;8(5):423–434. doi: 10.1136/gut.8.5.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. O'Brien M. J., Zamcheck N., Burke B., Kirkham S. E., Saravis C. A., Gottlieb L. S. Immunocytochemical localization of carcinoembryonic antigen in benign and malignant colorectal tissues. Assessment of diagnostic value. Am J Clin Pathol. 1981 Mar;75(3):283–290. doi: 10.1093/ajcp/75.3.283. [DOI] [PubMed] [Google Scholar]
  16. Poger M. E., Hirsch B. R., Lamm M. E. Synthesis of secretory component by colonic neoplasms. Am J Pathol. 1976 Feb;82(2):327–338. [PMC free article] [PubMed] [Google Scholar]
  17. Poger M. E., Lamm M. E. Localization of free and bound secretory component in human intestinal epithelial cells. A model for the assembly of secretory IgA. J Exp Med. 1974 Mar 1;139(3):629–642. doi: 10.1084/jem.139.3.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Primus F. J., Clark C. A., Goldenburg D. M. Immunoperoxidase localization of carcinoembryonic antigen in normal human intestinal mucosa. J Natl Cancer Inst. 1981 Nov;67(5):1031–1039. [PubMed] [Google Scholar]
  19. Riddell R. H., Goldman H., Ransohoff D. F., Appelman H. D., Fenoglio C. M., Haggitt R. C., Ahren C., Correa P., Hamilton S. R., Morson B. C. Dysplasia in inflammatory bowel disease: standardized classification with provisional clinical applications. Hum Pathol. 1983 Nov;14(11):931–968. doi: 10.1016/s0046-8177(83)80175-0. [DOI] [PubMed] [Google Scholar]
  20. Rognum T. O., Elgjo K., Fausa O., Brandtzaeg P. Immunohistochemical evaluation of carcinoembryonic antigen, secretory component, and epithelial IgA in ulcerative colitis with dysplasia. Gut. 1982 Feb;23(2):123–133. doi: 10.1136/gut.23.2.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rognum T., Elgjo K., Brandtzaeg P., Orjasaeter H., Bergan A. Plasma carcinoembryonic antigen concentrations and immunohistochemical patterns of epithelial marker antigens in patients with large bowel carcinoma. J Clin Pathol. 1982 Sep;35(9):922–933. doi: 10.1136/jcp.35.9.922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sun N. C., Edgington T. D., Carpentier C. L., McAfee W., Terry R., Bateman J. Immunohistochemical localization of carcinoembryonic antigen (CEA), CEA-S, and nonspecific cross-reacting antigen (NCA) in carcinomas of lung. Cancer. 1983 Nov 1;52(9):1632–1641. doi: 10.1002/1097-0142(19831101)52:9<1632::aid-cncr2820520915>3.0.co;2-q. [DOI] [PubMed] [Google Scholar]
  23. WARREN S., SOMMERS S. C. Pathogenesis of ulcerative colitis. Am J Pathol. 1949 Jul;25(4):657–679. [PMC free article] [PubMed] [Google Scholar]
  24. Wagener C., Hain F., Breuer H., Olude S., Cremer H., Müller-Wallraf R. Immunohistochemical demonstration of carcinoembryonic antigen in normal, transitional and inflamed colonic mucosa. Oncodev Biol Med. 1981;2(5):331–343. [PubMed] [Google Scholar]
  25. Weisz-Carrington P., Poger M. E., Lamm M. E. Secretory immunoglobulins in colonic neoplasms. Am J Pathol. 1976 Nov;85(2):303–316. [PMC free article] [PubMed] [Google Scholar]
  26. Yardley J. H., Keren D. F. "Precancer" lesions in ulcerative colits. A retrospective study of rectal biopsy and colectomy specimens. Cancer. 1974 Sep;34(3):suppl–suppl:844. doi: 10.1002/1097-0142(197409)34:3+<835::aid-cncr2820340709>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES