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. 1997 Jan;50(1):37–39. doi: 10.1136/jcp.50.1.37

Paediatric rhabdomyosarcoma: MyoD1 demonstration in routinely processed tissue sections using wet heat pretreatment (pressure cooking) for antigen retrieval.

M E Engel 1, S C Mouton 1, M Emms 1
PMCID: PMC499710  PMID: 9059354

Abstract

AIMS: To investigate wet heat pretreatment (pressure cooking) as a means of antigen retrieval for demonstration of MyoD1 in paraffin wax embedded tissue. METHODS: Routinely processed tissue sections of transmission electron microscope confirmed cases of rhabdomyosarcoma were stained immunohistochemically with the MyoD1 antibody. Antigen retrieval was achieved by wet heat pretreatment of the tissue sections. RESULTS: MyoD1 was stained successfully in all seven cases. The protein was localised to nuclei and cytoplasm depending on the type of tumour cell. CONCLUSIONS: Wet heat pretreatment for antigen retrieval from routinely processed tissue sections permits excellent subsequent immunostaining for MyoD1 in rhabdomyoblasts.

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Selected References

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  1. Bankfalvi A., Navabi H., Bier B., Böcker W., Jasani B., Schmid K. W. Wet autoclave pretreatment for antigen retrieval in diagnostic immunohistochemistry. J Pathol. 1994 Nov;174(3):223–228. doi: 10.1002/path.1711740312. [DOI] [PubMed] [Google Scholar]
  2. Bengal E., Ransone L., Scharfmann R., Dwarki V. J., Tapscott S. J., Weintraub H., Verma I. M. Functional antagonism between c-Jun and MyoD proteins: a direct physical association. Cell. 1992 Feb 7;68(3):507–519. doi: 10.1016/0092-8674(92)90187-h. [DOI] [PubMed] [Google Scholar]
  3. Carter R. L., McCarthy K. P. Divergent differentiation in round-cell tumours of soft tissues: an interim appraisal. Histopathology. 1993 Jul;23(1):93–97. doi: 10.1111/j.1365-2559.1993.tb01191.x. [DOI] [PubMed] [Google Scholar]
  4. Cuevas E. C., Bateman A. C., Wilkins B. S., Johnson P. A., Williams J. H., Lee A. H., Jones D. B., Wright D. H. Microwave antigen retrieval in immunocytochemistry: a study of 80 antibodies. J Clin Pathol. 1994 May;47(5):448–452. doi: 10.1136/jcp.47.5.448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dias P., Dilling M., Houghton P. The molecular basis of skeletal muscle differentiation. Semin Diagn Pathol. 1994 Feb;11(1):3–14. [PubMed] [Google Scholar]
  6. Norton A. J., Jordan S., Yeomans P. Brief, high-temperature heat denaturation (pressure cooking): a simple and effective method of antigen retrieval for routinely processed tissues. J Pathol. 1994 Aug;173(4):371–379. doi: 10.1002/path.1711730413. [DOI] [PubMed] [Google Scholar]
  7. Parham D. M. The molecular biology of childhood rhabdomyosarcoma. Semin Diagn Pathol. 1994 Feb;11(1):39–46. [PubMed] [Google Scholar]
  8. Peterson C. A., Gordon H., Hall Z. W., Paterson B. M., Blau H. M. Negative control of the helix-loop-helix family of myogenic regulators in the NFB mutant. Cell. 1990 Aug 10;62(3):493–502. doi: 10.1016/0092-8674(90)90014-6. [DOI] [PubMed] [Google Scholar]
  9. Powell C. Pressure cooking retrieves "lost" antigens in tissue sections. Wet autoclave pretreatment for antigen retrieval in diagnostic immunohistochemistry. Hum Exp Toxicol. 1995 May;14(5):465–466. doi: 10.1177/096032719501400516. [DOI] [PubMed] [Google Scholar]
  10. Shi S. R., Key M. E., Kalra K. L. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem. 1991 Jun;39(6):741–748. doi: 10.1177/39.6.1709656. [DOI] [PubMed] [Google Scholar]
  11. Tonin P. N., Scrable H., Shimada H., Cavenee W. K. Muscle-specific gene expression in rhabdomyosarcomas and stages of human fetal skeletal muscle development. Cancer Res. 1991 Oct 1;51(19):5100–5106. [PubMed] [Google Scholar]
  12. Tsokos M. The diagnosis and classification of childhood rhabdomyosarcoma. Semin Diagn Pathol. 1994 Feb;11(1):26–38. [PubMed] [Google Scholar]
  13. Weintraub H., Davis R., Tapscott S., Thayer M., Krause M., Benezra R., Blackwell T. K., Turner D., Rupp R., Hollenberg S. The myoD gene family: nodal point during specification of the muscle cell lineage. Science. 1991 Feb 15;251(4995):761–766. doi: 10.1126/science.1846704. [DOI] [PubMed] [Google Scholar]
  14. Wesche W. A., Fletcher C. D., Dias P., Houghton P. J., Parham D. M. Immunohistochemistry of MyoD1 in adult pleomorphic soft tissue sarcomas. Am J Surg Pathol. 1995 Mar;19(3):261–269. doi: 10.1097/00000478-199503000-00003. [DOI] [PubMed] [Google Scholar]

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