Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1986 May;39(5):542–546. doi: 10.1136/jcp.39.5.542

Prognostic importance of DNA flow cytometry in non-Hodgkin's lymphomas.

J C Macartney, R S Camplejohn, J Alder, M G Stone, G Powell
PMCID: PMC499917  PMID: 3722408

Abstract

Eighty one cases of non-Hodgkin's lymphoma were examined by DNA flow cytometry, using fixed embedded histological tissue. The frequency of detection of DNA aneuploidy and the values for S phase fractions depended on the histological subtype and grade of lymphoma. Twenty two of the patients with low grade centroblastic/centrocytic non-Hodgkin's lymphoma had repeat biopsies. Eleven of these patients remained histologically and cytometrically stable, but the remaining eleven transformed into high grade non-Hodgkin's lymphoma. The mean value for the S phase fraction in the initial biopsy specimens from patients which transformed was higher than that for patients whose lymphomas remained stable (p less than 0.001). It is proposed that estimates of S phase fraction prospectively identify patients with low grade non-Hodgkin's lymphoma at risk from transformation.

Full text

PDF
544

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baisch H., Göhde W., Linden W. A. Analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle. Radiat Environ Biophys. 1975 Jun 13;12(1):31–39. doi: 10.1007/BF02339807. [DOI] [PubMed] [Google Scholar]
  2. Barlogie B., Raber M. N., Schumann J., Johnson T. S., Drewinko B., Swartzendruber D. E., Göhde W., Andreeff M., Freireich E. J. Flow cytometry in clinical cancer research. Cancer Res. 1983 Sep;43(9):3982–3997. [PubMed] [Google Scholar]
  3. Braylan R. C., Benson N. A., Nourse V. A. Cellular DNA of human neoplastic B-cells measured by flow cytometry. Cancer Res. 1984 Nov;44(11):5010–5016. [PubMed] [Google Scholar]
  4. Braylan R. C., Diamond L. W., Powell M. L., Harty-Golder B. Percentage of cells in the S phase of the cell cycle in human lymphoma determined by flow cytometry. Cytometry. 1980 Nov;1(3):171–174. doi: 10.1002/cyto.990010302. [DOI] [PubMed] [Google Scholar]
  5. Braylan R. C., Fowlkes B. J., Jaffe E. S., Sanders S. K., Berard C. W., Herman C. J. Cell volumes and DNA distributions of normal and neoplastic human lymphoid cells. Cancer. 1978 Jan;41(1):201–209. doi: 10.1002/1097-0142(197801)41:1<201::aid-cncr2820410129>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  6. Camplejohn R. S., Macartney J. C. Comparison of DNA flow cytometry from fresh and paraffin embedded samples of non-Hodgkin's lymphoma. J Clin Pathol. 1985 Oct;38(10):1096–1099. doi: 10.1136/jcp.38.10.1096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cooper E. H., Peckham M. J., Millard R. E., Hamlin I. M., Gérard-Marchant R. Cell proliferation in human malignant lymphomas. Analysis of labelling index and DNA content in cell populations obtained by biopsy. Eur J Cancer. 1968 Jul;4(3):287–296. doi: 10.1016/0014-2964(68)90054-6. [DOI] [PubMed] [Google Scholar]
  8. Costa A., Bonadonna G., Villa E., Valagussa P., Silvestrini R. Labeling index as a prognostic marker in non-Hodgkin's lymphomas. J Natl Cancer Inst. 1981 Jan;66(1):1–5. [PubMed] [Google Scholar]
  9. Costa A., Mazzini G., Del Bino G., Silvestrini R. DNA content and kinetic characteristics of non-Hodgkin's lymphoma: determined by flow cytometry and autoradiography. Cytometry. 1981 Nov;2(3):185–188. doi: 10.1002/cyto.990020310. [DOI] [PubMed] [Google Scholar]
  10. Diamond L. W., Braylan R. C. Flow analysis of DNA content and cell size in non-Hodgkin's lymphoma. Cancer Res. 1980 Mar;40(3):703–712. [PubMed] [Google Scholar]
  11. Diamond L. W., Nathwani B. N., Rappaport H. Flow cytometry in the diagnosis and classification of malignant lymphoma and leukemia. Cancer. 1982 Sep 15;50(6):1122–1135. doi: 10.1002/1097-0142(19820915)50:6<1122::aid-cncr2820500616>3.0.co;2-q. [DOI] [PubMed] [Google Scholar]
  12. Frankfurt O. S., Greco W. R., Slocum H. K., Arbuck S. G., Gamarra M., Pavelic Z. P., Rustum Y. M. Proliferative characteristics of primary and metastatic human solid tumors by DNA flow cytometry. Cytometry. 1984 Nov;5(6):629–635. doi: 10.1002/cyto.990050612. [DOI] [PubMed] [Google Scholar]
  13. Gerdes J., Dallenbach F., Lennert K., Lemke H., Stein H. Growth fractions in malignant non-Hodgkin's lymphomas (NHL) as determined in situ with the monoclonal antibody Ki-67. Hematol Oncol. 1984 Oct-Dec;2(4):365–371. doi: 10.1002/hon.2900020406. [DOI] [PubMed] [Google Scholar]
  14. Habeshaw J. A., Lister T. A., Stansfeld A. G., Greaves M. F. Correlation of transferrin receptor expression with histological class and outcome in non-Hodgkin lymphoma. Lancet. 1983 Mar 5;1(8323):498–501. doi: 10.1016/s0140-6736(83)92191-8. [DOI] [PubMed] [Google Scholar]
  15. Hedley D. W., Friedlander M. L., Taylor I. W., Rugg C. A., Musgrove E. A. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem. 1983 Nov;31(11):1333–1335. doi: 10.1177/31.11.6619538. [DOI] [PubMed] [Google Scholar]
  16. Herrmann R., Barcos M., Stutzman L., Walsh D., Freeman A., Sokal J., Henderson E. S. The influence of histologic type on the incidence and duration of response in non-Hodgkin's lymphoma. Cancer. 1982 Jan 15;49(2):314–322. doi: 10.1002/1097-0142(19820115)49:2<314::aid-cncr2820490218>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  17. Kvaløy S., Godal T., Marton P. F., Steen H., Brenhovd I. O., Abrahamsen A. F. Spontaneous (H)-thymidine uptake in histological subgroups of human B-cell lymphomas. Scand J Haematol. 1981 Mar;26(3):221–234. doi: 10.1111/j.1600-0609.1981.tb01650.x. [DOI] [PubMed] [Google Scholar]
  18. Leonard R. C., Cuzick J., MacLennan I. C., Vanhegan R. I., Mackie P. H., McCormick C. V. Prognostic factors in non-Hodgkin's lymphoma: the importance of symptomatic stage as an adjunct to the Kiel histopathological classification. Br J Cancer. 1983 Jan;47(1):91–102. doi: 10.1038/bjc.1983.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lukes R. J., Collins R. D. Immunologic characterization of human malignant lymphomas. Cancer. 1974 Oct;34(4 Suppl):suppl–suppl:1503. doi: 10.1002/1097-0142(197410)34:8+<1488::aid-cncr2820340822>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  20. Molenaar W. M., Bartels H., Koudstaal J. Histological, epidemiological and clinical aspects of centroblastic-centrocytic lymphomas subdivided according to the "working formulation". Br J Cancer. 1984 Mar;49(3):263–268. doi: 10.1038/bjc.1984.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Oviatt D. L., Cousar J. B., Collins R. D., Flexner J. M., Stein R. S. Malignant lymphomas of follicular center cell origin in humans. V. Incidence, clinical features, and prognostic implications of transformation of small cleaved cell nodular lymphoma. Cancer. 1984 Mar 1;53(5):1109–1114. doi: 10.1002/1097-0142(19840301)53:5<1109::aid-cncr2820530516>3.0.co;2-o. [DOI] [PubMed] [Google Scholar]
  22. Shackney S. E., Skramstad K. S., Cunningham R. E., Dugas D. J., Lincoln T. L., Lukes R. J. Dual parameter flow cytometry studies in human lymphomas. J Clin Invest. 1980 Dec;66(6):1281–1294. doi: 10.1172/JCI109980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Silvestrini R., Piazza R., Riccardi A., Rilke F. Correlation of cell kinetic findings with morphology of non-Hodgkin's malignant lymphomas. J Natl Cancer Inst. 1977 Mar;58(3):499–504. doi: 10.1093/jnci/58.3.499. [DOI] [PubMed] [Google Scholar]
  24. Simonsson B., Nilsson K. 3H-thymidine uptake in chronic lymphocytic leukaemia cells. Scand J Haematol. 1980 Feb;24(2):169–173. doi: 10.1111/j.1600-0609.1980.tb02363.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES