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. 1992 Sep 15;89(18):8769–8773. doi: 10.1073/pnas.89.18.8769

Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit.

M Zhang 1, P D Zamore 1, M Carmo-Fonseca 1, A I Lamond 1, M R Green 1
PMCID: PMC50002  PMID: 1388271

Abstract

U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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