Abstract
Faecal samples from 37 patients with cystic fibrosis and 40 control patients at the Brompton Hospital and the London Chest Hospital were examined for the presence of Clostridium difficile. The organism was isolated from 2 (17%) of control patients who were receiving antibiotics and from one (3.6%) of control patients who had no antimicrobial treatment. Thirty two per cent of the patients with cystic fibrosis excreted C difficile, though none of them had diarrhoea. Two of the three isolates from control patients and nine of the 12 isolates from patients with cystic fibrosis produced toxin B (cytotoxin) in vitro. Toxin B was present in the stools of one of the control patients and three of the patients with cystic fibrosis; toxin A (enterotoxin) was not detected in the faeces of the patients with cystic fibrosis. Two cytotoxigenic strains of C difficile isolated from patients with cystic fibrosis were examined in hamsters; both were virulent, and the animals died.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Borriello S. P., Barclay F. E. An in-vitro model of colonisation resistance to Clostridium difficile infection. J Med Microbiol. 1986 Jun;21(4):299–309. doi: 10.1099/00222615-21-4-299. [DOI] [PubMed] [Google Scholar]
- Borriello S. P., Barclay F. E. Protection of hamsters against Clostridium difficile ileocaecitis by prior colonisation with non-pathogenic strains. J Med Microbiol. 1985 Jun;19(3):339–350. doi: 10.1099/00222615-19-3-339. [DOI] [PubMed] [Google Scholar]
- George W. L., Rolfe R. D., Finegold S. M. Clostridium difficile and its cytotoxin in feces of patients with antimicrobial agent-associated diarrhea and miscellaneous conditions. J Clin Microbiol. 1982 Jun;15(6):1049–1053. doi: 10.1128/jcm.15.6.1049-1053.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- George W. L., Sutter V. L., Citron D., Finegold S. M. Selective and differential medium for isolation of Clostridium difficile. J Clin Microbiol. 1979 Feb;9(2):214–219. doi: 10.1128/jcm.9.2.214-219.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haslam S. C., Ketley J. M., Mitchell T. J., Stephen J., Burdon D. W., Candy D. C. Growth of Clostridium difficile and production of toxins A and B in complex and defined media. J Med Microbiol. 1986 Jun;21(4):293–297. doi: 10.1099/00222615-21-4-293. [DOI] [PubMed] [Google Scholar]
- Larson H. E., Barclay F. E., Honour P., Hill I. D. Epidemiology of Clostridium difficile in infants. J Infect Dis. 1982 Dec;146(6):727–733. doi: 10.1093/infdis/146.6.727. [DOI] [PubMed] [Google Scholar]
- Larson H. E., Price A. B., Borriello S. P. Epidemiology of experimental enterocecitis due to Clostridium difficile. J Infect Dis. 1980 Sep;142(3):408–413. doi: 10.1093/infdis/142.3.408. [DOI] [PubMed] [Google Scholar]
- Larson H. E., Price A. B. Pseudomembranous colitis: Presence of clostridial toxin. Lancet. 1977 Dec 24;2(8052-8053):1312–1314. doi: 10.1016/s0140-6736(77)90363-4. [DOI] [PubMed] [Google Scholar]
- Peters T. J. Investigation of tissue organelles by a combination of analytical subcellular fractionation and enzymic microanalysis: a new approach to pathology. J Clin Pathol. 1981 Jan;34(1):1–12. doi: 10.1136/jcp.34.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Viscidi R., Willey S., Bartlett J. G. Isolation rates and toxigenic potential of Clostridium difficile isolates from various patient populations. Gastroenterology. 1981 Jul;81(1):5–9. [PubMed] [Google Scholar]
- Welkon C. J., Long S. S., Thompson C. M., Jr, Gilligan P. H. Clostridium difficile in patients with cystic fibrosis. Am J Dis Child. 1985 Aug;139(8):805–808. doi: 10.1001/archpedi.1985.02140100067032. [DOI] [PubMed] [Google Scholar]