Abstract
AIM: To assess the ability of the commercially available monoclonal antibody 1B12 (BioGenex, San Ramon, USA) to identify C albicans in formalin fixed, paraffin wax embedded material (FFPE). METHODS: Broth cultures of 20 strains of seven Candida species were resuspended in 4% agarose blocks, fixed in formalin for 24 hours, and embedded in paraffin wax. In addition, 16 blocks of FFPE tissue known to contain periodic acid-Schiff positive fungal hyphae were examined. Antigen retrieval involved microwave treatment of specimens in citrate buffer (0.01 M; pH 6.5) before addition of 1B12 antibody for 24 hours. Bound antibody was subsequently detected using a biotinylated link antibody and a peroxidase conjugated streptavidin. RESULTS: Only C albicans strains were 1B12 positive in the agarose blocks. All FFPE tissue blocks were found to contain 1B12 positive hyphal structures, indicating the presence of C albicans. CONCLUSIONS: The ability to identify candida organisms penetrating the lesional tissue in cases of chronic hyperplastic candidosis will help to clarify the role of individual Candida spp in this important form of oral candidosis.
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- Barrett A. W., Kingsmill V. J., Speight P. M. The frequency of fungal infection in biopsies of oral mucosal lesions. Oral Dis. 1998 Mar;4(1):26–31. doi: 10.1111/j.1601-0825.1998.tb00251.x. [DOI] [PubMed] [Google Scholar]
- Krogh P. The role of yeasts in oral cancer by means of endogenous nitrosation. Acta Odontol Scand. 1990 Feb;48(1):85–88. doi: 10.3109/00016359009012738. [DOI] [PubMed] [Google Scholar]
- Monteagudo C., Marcilla A., Mormeneo S., Llombart-Bosch A., Sentandreu R. Specific immunohistochemical identification of Candida albicans in paraffin-embedded tissue with a new monoclonal antibody (1B12). Am J Clin Pathol. 1995 Feb;103(2):130–135. doi: 10.1093/ajcp/103.2.130. [DOI] [PubMed] [Google Scholar]
- TASCHDJIAN C. L., REISS F., KOZINN P. J. Experimental vaginal candidiasis in mice; its implications for superficial candidiasis in humans. J Invest Dermatol. 1960 Feb;34:89–94. [PubMed] [Google Scholar]