Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1999 Mar;52(3):234–236. doi: 10.1136/jcp.52.3.234

Telomerase expression in intracranial tumours: prognostic potential for malignant gliomas and meningiomas.

M L Falchetti 1, R Pallini 1, L M Larocca 1, R Verna 1, E D'Ambrosio 1
PMCID: PMC501091  PMID: 10450187

Abstract

AIM: To evaluate the diagnostic value of telomerase expression in intracranial tumours. METHODS: 98 surgical specimens from different neoplasms were analysed by the telomeric repeat amplification protocol (TRAP) and the presence of telomerase compared with the histological diagnosis and the proliferation index. RESULTS: A high degree of positivity for telomerase activity was found in glioblastomas and atypical/anaplastic meningiomas. Telomerase activity was poorly detected in anaplastic astrocytomas. CONCLUSIONS: The TRAP assay seems to be a valuable index for identifying meningeal tumours with aggressive behaviour.

Full text

PDF
236

Images in this article

235Image
on p.235

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avilion A. A., Piatyszek M. A., Gupta J., Shay J. W., Bacchetti S., Greider C. W. Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues. Cancer Res. 1996 Feb 1;56(3):645–650. [PubMed] [Google Scholar]
  2. Falchetti M. L., Levi A., Molinari P., Verna R., D'Ambrosio E. Increased sensitivity and reproducibility of TRAP assay by avoiding direct primers interaction. Nucleic Acids Res. 1998 Feb 1;26(3):862–863. doi: 10.1093/nar/26.3.862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Harris N. L., Jaffe E. S., Stein H., Banks P. M., Chan J. K., Cleary M. L., Delsol G., De Wolf-Peeters C., Falini B., Gatter K. C. A revised European-American classification of lymphoid neoplasms: a proposal from the International Lymphoma Study Group. Blood. 1994 Sep 1;84(5):1361–1392. [PubMed] [Google Scholar]
  4. Hiyama E., Hiyama K., Yokoyama T., Matsuura Y., Piatyszek M. A., Shay J. W. Correlating telomerase activity levels with human neuroblastoma outcomes. Nat Med. 1995 Mar;1(3):249–255. doi: 10.1038/nm0395-249. [DOI] [PubMed] [Google Scholar]
  5. Hiyama E., Yokoyama T., Tatsumoto N., Hiyama K., Imamura Y., Murakami Y., Kodama T., Piatyszek M. A., Shay J. W., Matsuura Y. Telomerase activity in gastric cancer. Cancer Res. 1995 Aug 1;55(15):3258–3262. [PubMed] [Google Scholar]
  6. Kim N. W., Piatyszek M. A., Prowse K. R., Harley C. B., West M. D., Ho P. L., Coviello G. M., Wright W. E., Weinrich S. L., Shay J. W. Specific association of human telomerase activity with immortal cells and cancer. Science. 1994 Dec 23;266(5193):2011–2015. doi: 10.1126/science.7605428. [DOI] [PubMed] [Google Scholar]
  7. Kleihues P., Burger P. C., Scheithauer B. W. The new WHO classification of brain tumours. Brain Pathol. 1993 Jul;3(3):255–268. doi: 10.1111/j.1750-3639.1993.tb00752.x. [DOI] [PubMed] [Google Scholar]
  8. Langford L. A., Piatyszek M. A., Xu R., Schold S. C., Jr, Shay J. W. Telomerase activity in human brain tumours. Lancet. 1995 Nov 11;346(8985):1267–1268. doi: 10.1016/s0140-6736(95)91865-5. [DOI] [PubMed] [Google Scholar]
  9. Langford L. A., Piatyszek M. A., Xu R., Schold S. C., Jr, Wright W. E., Shay J. W. Telomerase activity in ordinary meningiomas predicts poor outcome. Hum Pathol. 1997 Apr;28(4):416–420. doi: 10.1016/s0046-8177(97)90029-0. [DOI] [PubMed] [Google Scholar]
  10. Watson J. D. Origin of concatemeric T7 DNA. Nat New Biol. 1972 Oct 18;239(94):197–201. doi: 10.1038/newbio239197a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES