Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1993 May;46(5):446–449. doi: 10.1136/jcp.46.5.446

Proliferating cell nuclear antigen (PCNA) and nucleolar organiser regions in Hodgkin's disease: correlation with morphology.

J Freeman 1, D B Kellock 1, C C Yu 1, J Crocker 1, D A Levison 1, P A Hall 1
PMCID: PMC501255  PMID: 8100573

Abstract

AIM--To define the distribution of proliferating cell nuclear antigen (PCNA) and silver staining nucleolar organiser regions (AgNORs) in Hodgkin's disease. METHODS--PCNA was shown in a series of 34 cases of Hodgkin's disease using immunohistochemical methods. In a second series of 46 cases the AgNOR technique for interphase nucleolar organiser regions was studied. Both series comprised routinely fixed and processed paraffin wax sections of three main Rye subtypes. RESULTS--In all cases, regardless of Rye subtype, most Sternberg-Reed cells and mononuclear Hodgkin cells showed nuclear PCNA immunoreactivity and such cells had 15 or more AgNOR sites. The Hodgkin cells had, in general, about half the number of AgNORs seen in Sternberg-Reed variants. CONCLUSIONS--These data support the notion that Hodgkin's disease can be regarded as a high grade lymphoma, the large Hodgkin's and Sternberg-Reed cells being the (PCNA positive and AgNOR rich) neoplastic elements with high proliferative capacity. A smaller proportion of the associated cells also showed evidence of proliferation.

Full text

PDF
446

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akerman M., Brandt L., Johnson A., Olsson H. Mitotic activity in non-Hodgkin's lymphoma. Relation to the Kiel classification and to prognosis. Br J Cancer. 1987 Feb;55(2):219–223. doi: 10.1038/bjc.1987.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anastasi J., Bauer K. D., Variakojis D. DNA aneuploidy in Hodgkin's disease. A multiparameter flow-cytometric analysis with cytologic correlation. Am J Pathol. 1987 Sep;128(3):573–582. [PMC free article] [PubMed] [Google Scholar]
  3. Benjamin D. R., Gown A. M. Aberrant cytoplasmic expression of proliferating cell nuclear antigen in Hodgkin's disease. Am J Surg Pathol. 1991 Aug;15(8):764–768. doi: 10.1097/00000478-199108000-00006. [DOI] [PubMed] [Google Scholar]
  4. Cooper E. H., Peckham M. J., Millard R. E., Hamlin I. M., Gérard-Marchant R. Cell proliferation in human malignant lymphomas. Analysis of labelling index and DNA content in cell populations obtained by biopsy. Eur J Cancer. 1968 Jul;4(3):287–296. doi: 10.1016/0014-2964(68)90054-6. [DOI] [PubMed] [Google Scholar]
  5. Crocker J., Macartney J. C., Smith P. J. Correlation between DNA flow cytometric and nucleolar organizer region data in non-Hodgkin's lymphomas. J Pathol. 1988 Feb;154(2):151–156. doi: 10.1002/path.1711540207. [DOI] [PubMed] [Google Scholar]
  6. Crocker J., Nar P. Nucleolar organizer regions in lymphomas. J Pathol. 1987 Feb;151(2):111–118. doi: 10.1002/path.1711510203. [DOI] [PubMed] [Google Scholar]
  7. Derenzini M., Pession A., Farabegoli F., Trerè D., Badiali M., Dehan P. Relationship between interphasic nucleolar organizer regions and growth rate in two neuroblastoma cell lines. Am J Pathol. 1989 Apr;134(4):925–932. [PMC free article] [PubMed] [Google Scholar]
  8. Diamond L. W., Nathwani B. N., Rappaport H. Flow cytometry in the diagnosis and classification of malignant lymphoma and leukemia. Cancer. 1982 Sep 15;50(6):1122–1135. doi: 10.1002/1097-0142(19820915)50:6<1122::aid-cncr2820500616>3.0.co;2-q. [DOI] [PubMed] [Google Scholar]
  9. Falini B., Stein H., Pileri S., Canino S., Farabbi R., Martelli M. F., Grignani F., Fagioli M., Minelli O., Ciani C. Expression of lymphoid-associated antigens on Hodgkin's and Reed-Sternberg cells of Hodgkin's disease. An immunocytochemical study on lymph node cytospins using monoclonal antibodies. Histopathology. 1987 Dec;11(12):1229–1242. doi: 10.1111/j.1365-2559.1987.tb01869.x. [DOI] [PubMed] [Google Scholar]
  10. Gerdes J., Dallenbach F., Lennert K., Lemke H., Stein H. Growth fractions in malignant non-Hodgkin's lymphomas (NHL) as determined in situ with the monoclonal antibody Ki-67. Hematol Oncol. 1984 Oct-Dec;2(4):365–371. doi: 10.1002/hon.2900020406. [DOI] [PubMed] [Google Scholar]
  11. Gerdes J., Van Baarlen J., Pileri S., Schwarting R., Van Unnik J. A., Stein H. Tumor cell growth fraction in Hodgkin's disease. Am J Pathol. 1987 Sep;128(3):390–393. [PMC free article] [PubMed] [Google Scholar]
  12. Hall P. A., Crocker J., Watts A., Stansfeld A. G. A comparison of nucleolar organizer region staining and Ki-67 immunostaining in non-Hodgkin's lymphoma. Histopathology. 1988 Apr;12(4):373–381. doi: 10.1111/j.1365-2559.1988.tb01952.x. [DOI] [PubMed] [Google Scholar]
  13. Hall P. A., Levison D. A. Review: assessment of cell proliferation in histological material. J Clin Pathol. 1990 Mar;43(3):184–192. doi: 10.1136/jcp.43.3.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hall P. A., Levison D. A., Woods A. L., Yu C. C., Kellock D. B., Watkins J. A., Barnes D. M., Gillett C. E., Camplejohn R., Dover R. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol. 1990 Dec;162(4):285–294. doi: 10.1002/path.1711620403. [DOI] [PubMed] [Google Scholar]
  15. Hall P. A., Richards M. A., Gregory W. M., d'Ardenne A. J., Lister T. A., Stansfeld A. G. The prognostic value of Ki67 immunostaining in non-Hodgkin's lymphoma. J Pathol. 1988 Mar;154(3):223–235. doi: 10.1002/path.1711540305. [DOI] [PubMed] [Google Scholar]
  16. Hall P. A., d'Ardenne A. J., Stansfeld A. G. Paraffin section immunohistochemistry. II. Hodgkin's disease and large cell anaplastic (Ki1) lymphoma. Histopathology. 1988 Aug;13(2):161–169. doi: 10.1111/j.1365-2559.1988.tb02021.x. [DOI] [PubMed] [Google Scholar]
  17. Howell W. M., Black D. A. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia. 1980 Aug 15;36(8):1014–1015. doi: 10.1007/BF01953855. [DOI] [PubMed] [Google Scholar]
  18. Hubbell H. R., Hsu T. C. Identification of nucleolus organizer regions (NORs) in normal and neoplastic human cells by the silver-staining technique. Cytogenet Cell Genet. 1977;19(4):185–196. doi: 10.1159/000130808. [DOI] [PubMed] [Google Scholar]
  19. Jan-Mohamed R. M., Armstrong S. J., Crocker J., Leyland M. J., Hulten M. A. The relationship between number of interphase nors and nor-bearing chromosomes in non-Hodgkin's lymphoma. J Pathol. 1989 May;158(1):3–7. doi: 10.1002/path.1711580103. [DOI] [PubMed] [Google Scholar]
  20. Kvaløy S., Marton P. F., Kaalhus O., Høie J., Foss-Abrahamsen A., Godal T. 3H-thymidine uptake in B cell lymphomas--relationship to treatment response and survival. Scand J Haematol. 1985 May;34(5):429–435. doi: 10.1111/j.1600-0609.1985.tb00773.x. [DOI] [PubMed] [Google Scholar]
  21. Morgan K. G., Quirke P., O'Brien C. J., Bird C. C. Hodgkin's disease: a flow cytometric study. J Clin Pathol. 1988 Apr;41(4):365–369. doi: 10.1136/jcp.41.4.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. PETRAKIS N. L., BOSTICK W. L., SIEGEL B. V. The deoxyribonucleic acid (DNA) content of Sternberg-Reed cells of Hodgkin's disease. J Natl Cancer Inst. 1959 Mar;22(3):551–554. [PubMed] [Google Scholar]
  23. Peckham M. J., Cooper E. H. Proliferation characteristics of the various classes of cells in Hodgkin's disease. Cancer. 1969 Jul;24(1):135–146. doi: 10.1002/1097-0142(196907)24:1<135::aid-cncr2820240118>3.0.co;2-h. [DOI] [PubMed] [Google Scholar]
  24. Ploton D., Menager M., Jeannesson P., Himber G., Pigeon F., Adnet J. J. Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level. Histochem J. 1986 Jan;18(1):5–14. doi: 10.1007/BF01676192. [DOI] [PubMed] [Google Scholar]
  25. Reeves B. R., Casey G., Harris H. Variations in the activity of nucleolar organizers in different tissues, demonstrated by silver staining of human normal and leukemic cells. Cancer Genet Cytogenet. 1982 Jul;6(3):223–230. doi: 10.1016/0165-4608(82)90059-0. [DOI] [PubMed] [Google Scholar]
  26. Silvestrini R., Piazza R., Riccardi A., Rilke F. Correlation of cell kinetic findings with morphology of non-Hodgkin's malignant lymphomas. J Natl Cancer Inst. 1977 Mar;58(3):499–504. doi: 10.1093/jnci/58.3.499. [DOI] [PubMed] [Google Scholar]
  27. Suresh U. R., Chawner L., Buckley C. H., Fox H. Do AgNOR counts reflect cellular ploidy or cellular proliferation? A study of trophoblastic tissue. J Pathol. 1990 Mar;160(3):213–215. doi: 10.1002/path.1711600306. [DOI] [PubMed] [Google Scholar]
  28. Waseem N. H., Lane D. P. Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form. J Cell Sci. 1990 May;96(Pt 1):121–129. doi: 10.1242/jcs.96.1.121. [DOI] [PubMed] [Google Scholar]
  29. Woods A. L., Hall P. A., Shepherd N. A., Hanby A. M., Waseem N. H., Lane D. P., Levison D. A. The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis. Histopathology. 1991 Jul;19(1):21–27. doi: 10.1111/j.1365-2559.1991.tb00890.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES