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. 1993 Aug;46(8):727–729. doi: 10.1136/jcp.46.8.727

Is high AgNOR quantity in hepatocytes associated with increased risk of hepatocellular carcinoma in chronic liver disease?

M Derenzini 1, D Trerè 1, F Oliveri 1, E David 1, P Colombatto 1, F Bonino 1, M R Brunetto 1
PMCID: PMC501457  PMID: 8408696

Abstract

AIMS--To evaluate whether high numbers of silver staining nucleolar organiser regions (AgNORs) in hepatocytes are associated with increased risk of hepatocellular carcinoma in chronic liver disease. METHODS--The quantitative distribution of AgNORs was studied in the liver biopsy specimens of 33 patients with chronic liver disease, 11 of whom developed hepatocellular carcinoma. The interval between liver biopsy and diagnosis of hepatocellular carcinoma was 26 months (range one to 61 months); the mean follow up of patients without hepatocellular carcinoma was 45 months (range 24-59 months). Quantitative evaluation of AgNORs was carried out on silver stained routine sections by morphometric analysis, using a computer assisted image analysis system. RESULTS--High interphase AgNOR values (> 3 microns2) were found in hepatocytes of nine out of the 11 (82%) patients in whom neoplastic transformation occurred. Of the remaining 22 patients, only seven (31%) had AgNOR values higher than > 3 microns2 (chi 2 4.83; p = 0.036). CONCLUSIONS--These results indicate that high numbers of interphase AgNORs are associated with increased risk of hepatocellular carcinoma in patients with chronic liver disease.

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Selected References

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  1. Colombo M., de Franchis R., Del Ninno E., Sangiovanni A., De Fazio C., Tommasini M., Donato M. F., Piva A., Di Carlo V., Dioguardi N. Hepatocellular carcinoma in Italian patients with cirrhosis. N Engl J Med. 1991 Sep 5;325(10):675–680. doi: 10.1056/NEJM199109053251002. [DOI] [PubMed] [Google Scholar]
  2. Crocker J., Macartney J. C., Smith P. J. Correlation between DNA flow cytometric and nucleolar organizer region data in non-Hodgkin's lymphomas. J Pathol. 1988 Feb;154(2):151–156. doi: 10.1002/path.1711540207. [DOI] [PubMed] [Google Scholar]
  3. Crocker J., McGovern J. Nucleolar organiser regions in normal, cirrhotic, and carcinomatous livers. J Clin Pathol. 1988 Oct;41(10):1044–1048. doi: 10.1136/jcp.41.10.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crocker J. Nucleolar organiser regions. Curr Top Pathol. 1990;82:91–149. doi: 10.1007/978-3-642-74668-0_3. [DOI] [PubMed] [Google Scholar]
  5. Derenzini M., Pession A., Trerè D. Quantity of nucleolar silver-stained proteins is related to proliferating activity in cancer cells. Lab Invest. 1990 Jul;63(1):137–140. [PubMed] [Google Scholar]
  6. Derenzini M., Thiry M., Goessens G. Ultrastructural cytochemistry of the mammalian cell nucleolus. J Histochem Cytochem. 1990 Sep;38(9):1237–1256. doi: 10.1177/38.9.2201735. [DOI] [PubMed] [Google Scholar]
  7. Giri D. D., Nottingham J. F., Lawry J., Dundas S. A., Underwood J. C. Silver-binding nucleolar organizer regions (AgNORs) in benign and malignant breast lesions: correlations with ploidy and growth phase by DNA flow cytometry. J Pathol. 1989 Apr;157(4):307–313. doi: 10.1002/path.1711570407. [DOI] [PubMed] [Google Scholar]
  8. Knodell R. G., Ishak K. G., Black W. C., Chen T. S., Craig R., Kaplowitz N., Kiernan T. W., Wollman J. Formulation and application of a numerical scoring system for assessing histological activity in asymptomatic chronic active hepatitis. Hepatology. 1981 Sep-Oct;1(5):431–435. doi: 10.1002/hep.1840010511. [DOI] [PubMed] [Google Scholar]
  9. Oliveri F., Brunetto M. R., Actis G. C., Bonino F. Pathobiology of chronic hepatitis virus infection and hepatocellular carcinoma (HCC). Ital J Gastroenterol. 1991 Nov;23(8):498–502. [PubMed] [Google Scholar]
  10. Orita T., Kajiwara K., Nishizaki T., Ikeda N., Kamiryo T., Aoki H. Nucleolar organizer regions in meningioma. Neurosurgery. 1990 Jan;26(1):43–46. doi: 10.1097/00006123-199001000-00006. [DOI] [PubMed] [Google Scholar]
  11. Pession A., Farabegoli F., Treré D., Novello F., Montanaro L., Sperti S., Rambelli F., Derenzini M. The Ag-NOR proteins and transcription and duplication of ribosomal genes in mammalian cell nucleoli. Chromosoma. 1991 May;100(4):242–250. doi: 10.1007/BF00344158. [DOI] [PubMed] [Google Scholar]
  12. Ploton D., Menager M., Jeannesson P., Himber G., Pigeon F., Adnet J. J. Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level. Histochem J. 1986 Jan;18(1):5–14. doi: 10.1007/BF01676192. [DOI] [PubMed] [Google Scholar]
  13. Tanaka T., Takeuchi T., Nishikawa A., Takami T., Mori H. Nucleolar organizer regions in hepatocarcinogenesis induced by N-2-fluorenylacetamide in rats: comparison with bromodeoxyuridine immunohistochemistry. Jpn J Cancer Res. 1989 Nov;80(11):1047–1051. doi: 10.1111/j.1349-7006.1989.tb02257.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Trerè D., Farabegoli F., Cancellieri A., Ceccarelli C., Eusebi V., Derenzini M. AgNOR area in interphase nuclei of human tumours correlates with the proliferative activity evaluated by bromodeoxyuridine labelling and Ki-67 immunostaining. J Pathol. 1991 Sep;165(1):53–59. doi: 10.1002/path.1711650109. [DOI] [PubMed] [Google Scholar]
  15. Trerè D., Pession A., Derenzini M. The silver-stained proteins of interphasic nucleolar organizer regions as a parameter of cell duplication rate. Exp Cell Res. 1989 Sep;184(1):131–137. doi: 10.1016/0014-4827(89)90371-6. [DOI] [PubMed] [Google Scholar]
  16. Verme G., Brunetto M. R., Oliveri F., Baldi M., Forzani B., Piantino P., Ponzetto A., Bonino F. Role of hepatitis delta virus infection in hepatocellular carcinoma. Dig Dis Sci. 1991 Aug;36(8):1134–1136. doi: 10.1007/BF01297460. [DOI] [PubMed] [Google Scholar]
  17. Zanetti A. Hepatitis B vaccination: an important method of preventing HBV-related hepatocellular carcinoma. Ital J Gastroenterol. 1992 Feb;24(2):100–102. [PubMed] [Google Scholar]

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