Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1994 Jul;47(7):601–604. doi: 10.1136/jcp.47.7.601

Assessment of cell proliferation in hydatidiform mole using monoclonal antibody MIB1 to Ki-67 antigen.

A N Cheung 1, H Y Ngan 1, R J Collins 1, Y L Wong 1
PMCID: PMC502073  PMID: 8089214

Abstract

AIMS--To assess the role of Ki67 immunoreactivity in predicting the clinical progress of hydatidiform mole. METHODS--Tissue from 87 hydatidiform moles, 11 normal first trimester placentas, 11 normal term placentas and 17 spontaneous abortions were examined for expression of Ki67 antigen, using the monoclonal antibody MIB1. RESULTS--Ki67 immunoreactivity was significantly higher in the tissue from normal first trimester placentas than in that from normal term placentas and spontaneous abortions. Among the 87 patients with hydatidiform moles studied, 20 developed persistent gestational trophoblastic disease and required subsequent treatment. There was no statistically significant difference in the Ki67 index between the 20 patients who developed persistent disease and those who did not. CONCLUSION--Hydatidiform moles which give rise to persistent trophoblastic disease do not have a higher proliferative rate than those which do not. The Ki67 index is not useful for predicting the prognosis of molar pregnancies.

Full text

PDF
601

Images in this article

602Image
on p.602
603Image
on p.603

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyd P. A. Quantitative structure of the normal human placenta from 10 weeks of gestation to term. Early Hum Dev. 1984 Jun;9(4):297–307. doi: 10.1016/0378-3782(84)90074-4. [DOI] [PubMed] [Google Scholar]
  2. Brewer J. I., Torok E. E., Kahan B. D., Stanhope C. R., Halpern B. Gestational trophoblastic disease: origin of choriocarcinoma, invasive mole and choriocarcinoma associated with hydatidiform mole, and some immunologic aspects. Adv Cancer Res. 1978;27:89–147. doi: 10.1016/s0065-230x(08)60931-8. [DOI] [PubMed] [Google Scholar]
  3. Bruno S., Darzynkiewicz Z. Cell cycle dependent expression and stability of the nuclear protein detected by Ki-67 antibody in HL-60 cells. Cell Prolif. 1992 Jan;25(1):31–40. doi: 10.1111/j.1365-2184.1992.tb01435.x. [DOI] [PubMed] [Google Scholar]
  4. Cattoretti G., Becker M. H., Key G., Duchrow M., Schlüter C., Galle J., Gerdes J. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol. 1992 Dec;168(4):357–363. doi: 10.1002/path.1711680404. [DOI] [PubMed] [Google Scholar]
  5. Cheung A. N., Ngan H. Y., Chen W. Z., Loke S. L., Collins R. J. The significance of proliferating cell nuclear antigen in human trophoblastic disease: an immunohistochemical study. Histopathology. 1993 Jun;22(6):565–568. doi: 10.1111/j.1365-2559.1993.tb00177.x. [DOI] [PubMed] [Google Scholar]
  6. Curry S. L., Hammond C. B., Tyrey L., Creasman W. T., Parker R. T. Hydatidiform mole: diagnosis, management, and long-term followup of 347 patients. Obstet Gynecol. 1975 Jan;45(1):1–8. [PubMed] [Google Scholar]
  7. Elston C. W., Bagshawe K. D. The value of histological grading in the management of hydatidiform mole. J Obstet Gynaecol Br Commonw. 1972 Aug;79(8):717–724. doi: 10.1111/j.1471-0528.1972.tb12907.x. [DOI] [PubMed] [Google Scholar]
  8. Geier G., Schuhmann R., Kraus H. Regional unterschiedliche Zellproliferation innerhalb der Plazentone reifer menschlicher Plazenten. Autoradiographische Untersuchungen. Arch Gynakol. 1975;218(1):31–37. doi: 10.1007/BF00672281. [DOI] [PubMed] [Google Scholar]
  9. Genest D. R., Laborde O., Berkowitz R. S., Goldstein D. P., Bernstein M. R., Lage J. A clinicopathologic study of 153 cases of complete hydatidiform mole (1980-1990): histologic grade lacks prognostic significance. Obstet Gynecol. 1991 Sep;78(3 Pt 1):402–409. [PubMed] [Google Scholar]
  10. Gerdes J. Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin Cancer Biol. 1990 Jun;1(3):199–206. [PubMed] [Google Scholar]
  11. Gerdes J., Lemke H., Baisch H., Wacker H. H., Schwab U., Stein H. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710–1715. [PubMed] [Google Scholar]
  12. Gerdes J., Schwab U., Lemke H., Stein H. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13–20. doi: 10.1002/ijc.2910310104. [DOI] [PubMed] [Google Scholar]
  13. Hall P. A., Levison D. A. Review: assessment of cell proliferation in histological material. J Clin Pathol. 1990 Mar;43(3):184–192. doi: 10.1136/jcp.43.3.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hall P. A., Richards M. A., Gregory W. M., d'Ardenne A. J., Lister T. A., Stansfeld A. G. The prognostic value of Ki67 immunostaining in non-Hodgkin's lymphoma. J Pathol. 1988 Mar;154(3):223–235. doi: 10.1002/path.1711540305. [DOI] [PubMed] [Google Scholar]
  15. McCormick D., Chong H., Hobbs C., Datta C., Hall P. A. Detection of the Ki-67 antigen in fixed and wax-embedded sections with the monoclonal antibody MIB1. Histopathology. 1993 Apr;22(4):355–360. doi: 10.1111/j.1365-2559.1993.tb00135.x. [DOI] [PubMed] [Google Scholar]
  16. Morrow C. P., Kletzky O. A., Disaia P. J., Townsend D. E., Mishell D. R., Nakamura R. M. Clinical and laboratory correlates of molar pregnancy and trophoblastic disease. Am J Obstet Gynecol. 1977 Jun 15;128(4):424–430. doi: 10.1016/0002-9378(77)90562-2. [DOI] [PubMed] [Google Scholar]
  17. Newlands E. S., Fisher R. A., Searle F. The immune system in disease: gestational trophoblastic tumours. Baillieres Clin Obstet Gynaecol. 1992 Sep;6(3):519–539. doi: 10.1016/s0950-3552(05)80009-4. [DOI] [PubMed] [Google Scholar]
  18. Quinn C. M., Wright N. A. The usefulness of clinical measurements of cell proliferation in gynecological cancer. Int J Gynecol Pathol. 1992;11(2):131–143. doi: 10.1097/00004347-199204000-00007. [DOI] [PubMed] [Google Scholar]
  19. Scott R. J., Hall P. A., Haldane J. S., van Noorden S., Price Y., Lane D. P., Wright N. A. A comparison of immunohistochemical markers of cell proliferation with experimentally determined growth fraction. J Pathol. 1991 Oct;165(2):173–178. doi: 10.1002/path.1711650213. [DOI] [PubMed] [Google Scholar]
  20. Suresh U. R., Hale R. J., Fox H., Buckley C. H. Use of proliferation cell nuclear antigen immunoreactivity for distinguishing hydropic abortions from partial hydatidiform moles. J Clin Pathol. 1993 Jan;46(1):48–50. doi: 10.1136/jcp.46.1.48. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Szulman A. E., Surti U. The syndromes of hydatidiform mole. I. Cytogenetic and morphologic correlations. Am J Obstet Gynecol. 1978 Jul 15;131(6):665–671. doi: 10.1016/0002-9378(78)90829-3. [DOI] [PubMed] [Google Scholar]
  22. Vaitukaitis J. L., Braunstein G. D., Ross G. T. A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone. Am J Obstet Gynecol. 1972 Jul 15;113(6):751–758. doi: 10.1016/0002-9378(72)90553-4. [DOI] [PubMed] [Google Scholar]
  23. Wolf H. K., Michalopoulos G. K. Proliferating cell nuclear antigen in human placenta and trophoblastic disease. Pediatr Pathol. 1992 Mar-Apr;12(2):147–154. doi: 10.3109/15513819209023291. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES