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. 1995 Jan;48(1):22–25. doi: 10.1136/jcp.48.1.22

Immunophenotypic analysis of childhood Burkitt's lymphoma in the West Midlands 1957-1986.

T J Jones 1, N A Coad 1, K R Muir 1, S E Parkes 1, C D Evans 1, J R Mann 1
PMCID: PMC502255  PMID: 7535803

Abstract

AIMS--To analyse the immunophenotype of a large number of non-endemic Burkitt's lymphomas to determine whether a B cell phenotype is consistently recognisable using formalin fixed, paraffin wax embedded archival material and a standard panel of commercially available antibodies. METHODS--Archival material was obtained from 30 cases of childhood Burkitt's lymphoma registered with the West Midlands Regional Children's Tumour Research Group. These were analysed by a standard avidin biotin complex immunoperoxidase method using antibodies to CD45, CD43, CD30, CD20, CD15, and immunoglobulin heavy and light chains. RESULTS--There was a high incidence of the CD45RB and CD20 immunophenotypes, with a clearly recognisable B cell lineage even in archival material. IgM was identifiable in 13 of the 23 (56.5%) cases tested. Only three of 17 (18%) cases expressed CD30. Positive membrane staining with CD45RO was observed in two (6.7%) cases. CONCLUSIONS--A B cell lineage can be identified in Burkitt's lymphoma in formalin fixed, paraffin wax embedded material, even in archival tissue. There was a low incidence of membrane staining with CD45RO which is a potential source of diagnostic confusion.

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Selected References

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  1. BURKITT D. A sarcoma involving the jaws in African children. Br J Surg. 1958 Nov;46(197):218–223. doi: 10.1002/bjs.18004619704. [DOI] [PubMed] [Google Scholar]
  2. Berard C. W., Jaffe E. S., Braylan R. C., Mann R. B., Nanba K. Immunologic aspects and pathology of the malignant lymphomas. Cancer. 1978 Aug;42(2 Suppl):911–921. doi: 10.1002/1097-0142(197808)42:2+<911::aid-cncr2820420713>3.0.co;2-g. [DOI] [PubMed] [Google Scholar]
  3. Brownell M. D., Sheibani K., Battifora H., Winberg C. D., Rappaport H. Distinction between undifferentiated (small noncleaved) and lymphoblastic lymphoma. An immunohistologic study on paraffin-embedded, fixed tissue sections. Am J Surg Pathol. 1987 Oct;11(10):779–787. doi: 10.1097/00000478-198710000-00005. [DOI] [PubMed] [Google Scholar]
  4. Davison A. M., McKinney P. A., Bailey C. C., Lewis I., Cartwright R. A., O'Brien C. Childhood lymphoma in Yorkshire. J Clin Pathol. 1992 Feb;45(2):130–134. doi: 10.1136/jcp.45.2.130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Garcia C. F., Weiss L. M., Warnke R. A. Small noncleaved cell lymphoma: an immunophenotypic study of 18 cases and comparison with large cell lymphoma. Hum Pathol. 1986 May;17(5):454–461. doi: 10.1016/s0046-8177(86)80034-x. [DOI] [PubMed] [Google Scholar]
  6. Grogan T. M., Warnke R. A., Kaplan H. S. A comparative study of Burkitt's and non-Burkitt's "undifferentiated" malignant lymphoma: immunologic, cytochemical, ultrastructural, cytologic, histopathologic, clinical and cell culture features. Cancer. 1982 May 1;49(9):1817–1828. doi: 10.1002/1097-0142(19820501)49:9<1817::aid-cncr2820490914>3.0.co;2-f. [DOI] [PubMed] [Google Scholar]
  7. Grogan T. M., Warnke R. A., Kaplan H. S. A comparative study of Burkitt's and non-Burkitt's "undifferentiated" malignant lymphoma: immunologic, cytochemical, ultrastructural, cytologic, histopathologic, clinical and cell culture features. Cancer. 1982 May 1;49(9):1817–1828. doi: 10.1002/1097-0142(19820501)49:9<1817::aid-cncr2820490914>3.0.co;2-f. [DOI] [PubMed] [Google Scholar]
  8. Gunvén P., Klein G., Klein E., Norin T., Singh S. Surface immunoglobulins on Burkitt's lymphoma biopsy cells from 91 patients. Int J Cancer. 1980 Jun 15;25(6):711–719. doi: 10.1002/ijc.2910250605. [DOI] [PubMed] [Google Scholar]
  9. Hall P. A., d'Ardenne A. J., Stansfeld A. G. Paraffin section immunohistochemistry. I. Non-Hodgkin's lymphoma. Histopathology. 1988 Aug;13(2):149–160. doi: 10.1111/j.1365-2559.1988.tb02020.x. [DOI] [PubMed] [Google Scholar]
  10. Hutchison R. E., Murphy S. B., Fairclough D. L., Shuster J. J., Sullivan M. P., Link M. P., Donaldson S. S., Berard C. W. Diffuse small noncleaved cell lymphoma in children, Burkitt's versus non-Burkitt's types. Results from the Pediatric Oncology Group and St. Jude Children's Research Hospital. Cancer. 1989 Jul 1;64(1):23–28. doi: 10.1002/1097-0142(19890701)64:1<23::aid-cncr2820640105>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]
  11. Kelly D. R., Nathwani B. N., Griffith R. C., Shuster J. J., Sullivan M. P., Hvizdala E., Murphy S. B., Berard C. W. A morphologic study of childhood lymphoma of the undifferentiated type. The Pediatric Oncology Group experience. Cancer. 1987 Mar 15;59(6):1132–1137. doi: 10.1002/1097-0142(19870315)59:6<1132::aid-cncr2820590616>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]
  12. Ladjadj Y., Philip T., Lenoir G. M., Tazerout F. Z., Bendisari K., Boukheloua R., Biron P., Brunat-Mentigny M., Aboulola M. Abdominal Burkitt-type lymphomas in Algeria. Br J Cancer. 1984 Apr;49(4):503–512. doi: 10.1038/bjc.1984.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lenoir G. M., Preud'homme J. L., Bernheim A., Berger R. Correlation between immunoglobulin light chain expression and variant translocation in Burkitt's lymphoma. Nature. 1982 Jul 29;298(5873):474–476. doi: 10.1038/298474a0. [DOI] [PubMed] [Google Scholar]
  14. Levine A. M., Pavlova Z., Pockros A. W., Parker J. W., Teitelbaum A. H., Paganini-Hill A., Powars D. R., Lukes R. J., Feinstein D. I. Small noncleaved follicular center cell (FCC) lymphoma: Burkitt and non-Burkitt variants in the United States. I. Clinical features. Cancer. 1983 Sep 15;52(6):1073–1079. doi: 10.1002/1097-0142(19830915)52:6<1073::aid-cncr2820520624>3.0.co;2-f. [DOI] [PubMed] [Google Scholar]
  15. Levine P. H., O'Conor G. T., Bernard C. W. Antibodies to Epstein-Barr virus (EBV) in American patients with Burkitt's lymphoma. Cancer. 1972 Sep;30(3):610–615. doi: 10.1002/1097-0142(197209)30:3<610::aid-cncr2820300303>3.0.co;2-a. [DOI] [PubMed] [Google Scholar]
  16. Magrath I. T., Freeman C. B., Pizzo P., Gadek J., Jaffe E., Santaella M., Hammer C., Frank M., Reaman G., Novikovs L. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J Natl Cancer Inst. 1980 Mar;64(3):477–483. [PubMed] [Google Scholar]
  17. Mann R. B., Jaffe E. S., Braylan R. C., Nanba K., Frank M. M., Ziegler J. L., Berard C. W. Non-endemic Burkitts's lymphoma. A B-cell tumor related to germinal centers. N Engl J Med. 1976 Sep 23;295(13):685–691. doi: 10.1056/NEJM197609232951301. [DOI] [PubMed] [Google Scholar]
  18. Miliauskas J. R., Berard C. W., Young R. C., Garvin A. J., Edwards B. K., DeVita V. T., Jr Undifferentiated non-Hodgkin's lymphomas (Burkitt's and non-Burkitt's types). The relevance of making this histologic distinction. Cancer. 1982 Nov 15;50(10):2115–2121. doi: 10.1002/1097-0142(19821115)50:10<2115::aid-cncr2820501024>3.0.co;2-9. [DOI] [PubMed] [Google Scholar]
  19. Norton A. J., Isaacson P. G. Lymphoma phenotyping in formalin-fixed and paraffin wax-embedded tissues. I. Range of antibodies and staining patterns. Histopathology. 1989 May;14(5):437–446. doi: 10.1111/j.1365-2559.1989.tb02180.x. [DOI] [PubMed] [Google Scholar]
  20. Norton A. J., Isaacson P. G. Lymphoma phenotyping in formalin-fixed and paraffin wax-embedded tissues: II. Profiles of reactivity in the various tumour types. Histopathology. 1989 Jun;14(6):557–579. doi: 10.1111/j.1365-2559.1989.tb02198.x. [DOI] [PubMed] [Google Scholar]
  21. O'CONOR G. T., RAPPAPORT H., SMITH E. B. CHILDHOOD LYMPHOMA RESEMBLING "BURKITT TUMOR" IN THE UNITED STATES. Cancer. 1965 Apr;18:411–417. doi: 10.1002/1097-0142(196504)18:4<411::aid-cncr2820180403>3.0.co;2-5. [DOI] [PubMed] [Google Scholar]
  22. Oliver J. D., Grogan T. M., Payne C. M., Spier C., Richter L. C., Rangel C. S. Burkitt's-like lymphoma of T-cell type. Mod Pathol. 1988 Jan;1(1):15–22. [PubMed] [Google Scholar]
  23. Pavlova Z., Parker J. W., Taylor C. R., Levine A. M., Feinstein D. I., Lukes R. J. Small noncleaved follicular center cell lymphoma: Burkitt's and non-Burkitt's variants in the US. II. Pathologic and immunologic features. Cancer. 1987 Jun 1;59(11):1892–1902. doi: 10.1002/1097-0142(19870601)59:11<1892::aid-cncr2820591109>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
  24. Payne C. M., Grogan T. M., Cromey D. W., Bjore C. G., Jr, Kerrigan D. P. An ultrastructural morphometric and immunophenotypic evaluation of Burkitt's and Burkitt's-like lymphomas. Lab Invest. 1987 Aug;57(2):200–218. [PubMed] [Google Scholar]
  25. Philip T., Lenoir G. M., Bryon P. A., Gerard-Marchant R., Souillet G., Philippe N., Freycon F., Brunat-Mentigny M. Burkitt-type lymphoma in France among non-Hodgkin malignant lymphomas in Caucasian children. Br J Cancer. 1982 May;45(5):670–678. doi: 10.1038/bjc.1982.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stansfeld A. G., Diebold J., Noel H., Kapanci Y., Rilke F., Kelényi G., Sundstrom C., Lennert K., van Unnik J. A., Mioduszewska O. Updated Kiel classification for lymphomas. Lancet. 1988 Feb 6;1(8580):292–293. doi: 10.1016/s0140-6736(88)90367-4. [DOI] [PubMed] [Google Scholar]
  27. Van Eyken P., De Wolf-Peeters C., Van den Oord J., Tricot G., Desmet V. Expression of leukocyte common antigen in lymphoblastic lymphoma and small noncleaved undifferentiated non-Burkitt's lymphoma: an immunohistochemical study. J Pathol. 1987 Apr;151(4):257–261. doi: 10.1002/path.1711510404. [DOI] [PubMed] [Google Scholar]
  28. Wilson J. F., Kjeldsberg C. R., Sposto R., Jenkin R. D., Chilcote R. R., Coccia P., Exelby R. R., Kersey J., Meadows A., Siegel S. The pathology of non-Hodgkin's lymphoma of childhood: II. Reproducibility and relevance of the histologic classification of "undifferentiated" lymphomas (Burkitt's versus non-Burkitt's). Hum Pathol. 1987 Oct;18(10):1008–1014. doi: 10.1016/s0046-8177(87)80217-4. [DOI] [PubMed] [Google Scholar]
  29. Wright D. H. Burkitt's tumour in England. A comparison with childhood lymphosarcoma. Int J Cancer. 1966 Sep 15;1(5):503–514. doi: 10.1002/ijc.2910010510. [DOI] [PubMed] [Google Scholar]

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