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. 1992 Nov 1;89(21):10331–10335. doi: 10.1073/pnas.89.21.10331

Alternative splicing generates metabotropic glutamate receptors inducing different patterns of calcium release in Xenopus oocytes.

J P Pin 1, C Waeber 1, L Prezeau 1, J Bockaert 1, S F Heinemann 1
PMCID: PMC50332  PMID: 1438218

Abstract

A splice variant of the metabotropic glutamate receptor (mGluR) 1a, named mGluR1c, was isolated. Compared to mGluR1a, the predicted mGluR1c protein is 302 amino acids shorter at its C-terminal end. Despite this difference, mGluR1c activates phospholipase C in Xenopus oocytes with a pharmacological profile identical to that of mGluR1a. However, in contrast to the large fast transient responses induced by mGluR1a, mGluR1c receptors elicit a small more slowly generated long-lasting oscillatory current, suggesting that these two receptors do not generate the same pattern of Ca2+ release in Xenopus oocytes. In situ hybridization data show that mGluR1c mRNA is expressed at a lower level than the other splice variants of mGluR1. Some differences in the regional distribution of these transcripts were observed in the cerebellum, the olfactory bulb, and the striatum.

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